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Literature summary extracted from

  • Moonmangmee, D.; Fujii, Y.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.; Adachi, O.
    Purification and characterization of membrane-bound quinoprotein cyclic alcohol dehydrogenase from Gluconobacter frateurii CHM 9 (2001), Biosci. Biotechnol. Biochem., 65, 2763-2772.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.7 1
-
Cyclopentanol pH 5.5, 25°C Gluconobacter frateurii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.7 membrane localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm Gluconobacter frateurii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.7 Ca2+ addition of pyrroloquinoline quinone and Ca2+ converts the apo-enzyme to the holoenzyme Gluconobacter frateurii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.7 83000
-
x * 83000, SDS-PAGE Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.7 additional information Gluconobacter frateurii the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible ?
-
?
1.1.5.7 additional information Gluconobacter frateurii CHM 9 the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.7 Gluconobacter frateurii
-
CHM 9
-
1.1.5.7 Gluconobacter frateurii CHM 9
-
CHM 9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.7
-
Gluconobacter frateurii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.7 44.3
-
-
Gluconobacter frateurii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.7 (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ?
-
ir
1.1.5.7 (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ?
-
ir
1.1.5.7 (2R,3R)-2,3-butanediol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 1,2-butanediol + pyrroloquinoline quinone 63% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 1,3-butanediol + pyrroloquinoline quinone 12% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 1,3-cyclopentanediol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 1,4-cyclohexanediol + pyrroloquinoline quinone 14% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 2,3-butanediol + pyrroloquinoline quinone 186% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 2,4-pentanediol + pyrroloquinoline quinone 16% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii butane-2-one + reduced pyrroloquinoline quinone
-
ir
1.1.5.7 2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 butane-2-one + reduced pyrroloquinoline quinone
-
ir
1.1.5.7 2-hexanol + pyrroloquinoline quinone 10% of the activity compared to cyclopentanol Gluconobacter frateurii 2-hexanone + pyrroloquinoline quinol
-
ir
1.1.5.7 2-methyl-2,4-pentanediol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 2-propanol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii acetone + pyrroloquinoline quinol
-
ir
1.1.5.7 3-pentanol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii 3-pentanone + pyrroloquinoline quinol
-
ir
1.1.5.7 cis-1,2-cyclohexanediol + pyrroloquinoline quinone 88% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 cis-1,2-cyclopentanediol + pyrroloquinoline quinone 181% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone 32% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 cyclobutanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclobutanone + pyrroloquinoline quinol
-
ir
1.1.5.7 cyclohexanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclohexanone + pyrroloquinoline quinol
-
ir
1.1.5.7 cyclooctanol + pyrroloquinoline quinone 137% of the activity compared to cyclopentanol Gluconobacter frateurii cyclooctanone + pyrroloquinoline quinol
-
ir
1.1.5.7 cyclopentanol + pyrroloquinoline quinone only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ Gluconobacter frateurii cyclopentanone + pyrroloquinoline quinol
-
ir
1.1.5.7 D-arabitol + pyrroloquinoline quinone 78% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 D-mannitol + pyrroloquinoline quinone 25% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 D-sorbitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 glycerol + pyrroloquinoline quinone 59% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 meso-erythritol + pyrroloquinoline quinone 100% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1.1.5.7 additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii ?
-
?
1.1.5.7 additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii ?
-
?
1.1.5.7 additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii CHM 9 ?
-
?
1.1.5.7 additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii CHM 9 ?
-
?
1.1.5.7 ribitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir

Subunits

EC Number Subunits Comment Organism
1.1.5.7 ? x * 83000, SDS-PAGE Gluconobacter frateurii

Synonyms

EC Number Synonyms Comment Organism
1.1.5.7 MCAD
-
Gluconobacter frateurii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.7 25
-
assay at Gluconobacter frateurii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.7 5.5
-
assay at Gluconobacter frateurii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.7 pyrroloquinoline quinone only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ Gluconobacter frateurii