EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.7 | 1 | - |
Cyclopentanol | pH 5.5, 25°C | Gluconobacter frateurii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.7 | membrane | localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm | Gluconobacter frateurii | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.7 | Ca2+ | addition of pyrroloquinoline quinone and Ca2+ converts the apo-enzyme to the holoenzyme | Gluconobacter frateurii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.5.7 | 83000 | - |
x * 83000, SDS-PAGE | Gluconobacter frateurii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.7 | additional information | Gluconobacter frateurii | the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible | ? | - |
? | |
1.1.5.7 | additional information | Gluconobacter frateurii CHM 9 | the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.7 | Gluconobacter frateurii | - |
CHM 9 | - |
1.1.5.7 | Gluconobacter frateurii CHM 9 | - |
CHM 9 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.7 | - |
Gluconobacter frateurii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.5.7 | 44.3 | - |
- |
Gluconobacter frateurii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.7 | (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone | 74% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | (1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone | 74% of the activity compared to cyclopentanol | Gluconobacter frateurii CHM 9 | ? | - |
ir | |
1.1.5.7 | (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone | 11% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | (1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone | 11% of the activity compared to cyclopentanol | Gluconobacter frateurii CHM 9 | ? | - |
ir | |
1.1.5.7 | (2R,3R)-2,3-butanediol + pyrroloquinoline quinone | 41% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 1,2-butanediol + pyrroloquinoline quinone | 63% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 1,3-butanediol + pyrroloquinoline quinone | 12% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 1,3-cyclopentanediol + pyrroloquinoline quinone | 73% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 1,4-cyclohexanediol + pyrroloquinoline quinone | 14% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 2,3-butanediol + pyrroloquinoline quinone | 186% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 2,4-pentanediol + pyrroloquinoline quinone | 16% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 2-butanol + pyrroloquinoline quinone | 41% of the activity compared to cyclopentanol | Gluconobacter frateurii | butane-2-one + reduced pyrroloquinoline quinone | - |
ir | |
1.1.5.7 | 2-butanol + pyrroloquinoline quinone | 41% of the activity compared to cyclopentanol | Gluconobacter frateurii CHM 9 | butane-2-one + reduced pyrroloquinoline quinone | - |
ir | |
1.1.5.7 | 2-hexanol + pyrroloquinoline quinone | 10% of the activity compared to cyclopentanol | Gluconobacter frateurii | 2-hexanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | 2-methyl-2,4-pentanediol + pyrroloquinoline quinone | 17% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | 2-propanol + pyrroloquinoline quinone | 17% of the activity compared to cyclopentanol | Gluconobacter frateurii | acetone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | 3-pentanol + pyrroloquinoline quinone | 74% of the activity compared to cyclopentanol | Gluconobacter frateurii | 3-pentanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | cis-1,2-cyclohexanediol + pyrroloquinoline quinone | 88% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | cis-1,2-cyclopentanediol + pyrroloquinoline quinone | 181% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone | 32% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | cyclobutanol + pyrroloquinoline quinone | 73% of the activity compared to cyclopentanol | Gluconobacter frateurii | cyclobutanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | cyclohexanol + pyrroloquinoline quinone | 73% of the activity compared to cyclopentanol | Gluconobacter frateurii | cyclohexanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | cyclooctanol + pyrroloquinoline quinone | 137% of the activity compared to cyclopentanol | Gluconobacter frateurii | cyclooctanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | cyclopentanol + pyrroloquinoline quinone | only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ | Gluconobacter frateurii | cyclopentanone + pyrroloquinoline quinol | - |
ir | |
1.1.5.7 | D-arabitol + pyrroloquinoline quinone | 78% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | D-mannitol + pyrroloquinoline quinone | 25% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | D-sorbitol + pyrroloquinoline quinone | 34% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | glycerol + pyrroloquinoline quinone | 59% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | meso-erythritol + pyrroloquinoline quinone | 100% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir | |
1.1.5.7 | additional information | the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible | Gluconobacter frateurii | ? | - |
? | |
1.1.5.7 | additional information | the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols | Gluconobacter frateurii | ? | - |
? | |
1.1.5.7 | additional information | the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible | Gluconobacter frateurii CHM 9 | ? | - |
? | |
1.1.5.7 | additional information | the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols | Gluconobacter frateurii CHM 9 | ? | - |
? | |
1.1.5.7 | ribitol + pyrroloquinoline quinone | 34% of the activity compared to cyclopentanol | Gluconobacter frateurii | ? | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.7 | ? | x * 83000, SDS-PAGE | Gluconobacter frateurii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.7 | MCAD | - |
Gluconobacter frateurii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.7 | 25 | - |
assay at | Gluconobacter frateurii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.7 | 5.5 | - |
assay at | Gluconobacter frateurii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.7 | pyrroloquinoline quinone | only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ | Gluconobacter frateurii |