EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.1 | expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
3.2.2.2 | - |
Saccharolobus solfataricus |
EC Number | General Stability | Organism |
---|---|---|
3.2.2.1 | IAG-NH is characterized by remarkable resistance to guanidinium chloride-induced unfolding | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.1 | EDTA | after 1 h incubation with EDTA (5 mM) the enzyme activity is reduced to less than 0.05% and is restored by addition of 20 mM CaCl2 | Saccharolobus solfataricus | |
3.2.2.2 | EDTA | - |
Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 0.06 | - |
adenosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.1 | 0.16 | - |
guanosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.1 | 0.36 | - |
Inosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.2 | 0.06 | - |
adenosine | - |
Saccharolobus solfataricus | |
3.2.2.2 | 0.16 | - |
guanosine | - |
Saccharolobus solfataricus | |
3.2.2.2 | 0.34 | - |
Inosine | - |
Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.1 | Ca2+ | IAG-NH is a Ca2+-dependent enzyme | Saccharolobus solfataricus | |
3.2.2.2 | Ca2+ | - |
Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 34325 | - |
2 * 34325, calculated from sequence of cDNA | Saccharolobus solfataricus |
3.2.2.1 | 35000 | - |
subunit, SDS-PAGE | Saccharolobus solfataricus |
3.2.2.1 | 68650 | - |
calculated from sequence of cDNA | Saccharolobus solfataricus |
3.2.2.2 | 34000 | - |
2 * 34000 Da, SDS-PAGE, predicted: 34325 Da/subunit, intersubunit disulfide bond | Saccharolobus solfataricus |
3.2.2.2 | 70000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.2.1 | dithiothreitol | after 1 h pre-incubation at temperatures until 80°C, the enzyme remains completely stable even at high concentrations of dithiothreitol (0.7 M) whereas it becomes susceptible to the effect of the reducing agent as the temperature raises, after 1 h pre-incubation in the presence of 0.5 M, 0.6 M and 0.7 M dithiothreitol, IAG-NH retains 70%, 60% and 21% activity respectively | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.1 | Saccharolobus solfataricus | - |
- |
- |
3.2.2.1 | Saccharolobus solfataricus P2 | - |
- |
- |
3.2.2.2 | Saccharolobus solfataricus | - |
- |
- |
3.2.2.2 | Saccharolobus solfataricus P2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.1 | methylthioinosine-Sepharose column chromatography | Saccharolobus solfataricus |
3.2.2.2 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.1 | adenosine + H2O | IAG-NH shows higher affinity for adenosine with respect to inosine and guanosine, but the catalytic efficiency of these substrates is comparable | Saccharolobus solfataricus | D-ribose + adenine | - |
? | |
3.2.2.1 | guanosine + H2O | highest activity | Saccharolobus solfataricus | D-ribose + guanine | - |
? | |
3.2.2.1 | guanosine + H2O | highest activity | Saccharolobus solfataricus P2 | D-ribose + guanine | - |
? | |
3.2.2.1 | inosine + H2O | - |
Saccharolobus solfataricus | D-ribose + hypoxanthine | - |
? | |
3.2.2.1 | additional information | IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+ | Saccharolobus solfataricus | ? | - |
? | |
3.2.2.1 | additional information | IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+ | Saccharolobus solfataricus P2 | ? | - |
? | |
3.2.2.2 | adenosine + H2O | - |
Saccharolobus solfataricus | adenine + D-ribose | - |
? | |
3.2.2.2 | adenosine + H2O | - |
Saccharolobus solfataricus P2 | adenine + D-ribose | - |
? | |
3.2.2.2 | guanosine + H2O | - |
Saccharolobus solfataricus | guanine + D-ribose | - |
? | |
3.2.2.2 | guanosine + H2O | - |
Saccharolobus solfataricus P2 | guanine + D-ribose | - |
? | |
3.2.2.2 | inosine + H2O | - |
Saccharolobus solfataricus | hypoxanthine + D-ribose | - |
? | |
3.2.2.2 | inosine + H2O | - |
Saccharolobus solfataricus P2 | hypoxanthine + D-ribose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.1 | homodimer | 2 * 34325, calculated from sequence of cDNA | Saccharolobus solfataricus |
3.2.2.2 | homodimer | 2 * 34000 Da, SDS-PAGE, predicted: 34325 Da/subunit, intersubunit disulfide bond | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.1 | IAG-NH | the enzyme is specific for adenosine, guanosine and inosine | Saccharolobus solfataricus |
3.2.2.1 | inosine-adenosine-guanosine nucleoside hydrolase | - |
Saccharolobus solfataricus |
3.2.2.1 | purine-specific ribonucleoside hydrolase | - |
Saccharolobus solfataricus |
3.2.2.2 | inosine-adenosine-guanosine nucleoside hydrolase | purine-specific | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 105 | - |
- |
Saccharolobus solfataricus |
3.2.2.2 | 105 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 60 | 115 | the enzyme is highly thermoactive, its activity increases sharply up to the optimal temperature of 105°C and 50% activity is still observed at 115°C | Saccharolobus solfataricus |
3.2.2.2 | 105 | 115 | 105°C: maximal activity, 115°C: 50% of maximal activity | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.1 | 100 | 110 | IAG-NH is characterized by extreme thermodynamic stability with a melting temperature at 107°C, it retains full activity after 1 h incubation at 90°C and shows half-lives of 45 min, 30 min and 15 min at 100°C, 105°C, and 110°C, respectively | Saccharolobus solfataricus |
3.2.2.2 | 90 | - |
100% activity after 1h, apparent melting temperature: 107°C | Saccharolobus solfataricus |
3.2.2.2 | 100 | - |
half-live 45 min | Saccharolobus solfataricus |
3.2.2.2 | 105 | - |
half-live 30 min | Saccharolobus solfataricus |
3.2.2.2 | 110 | - |
half-live 15 min | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.1 | 3.8 | - |
adenosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.1 | 23.3 | - |
Inosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.1 | 29.1 | - |
guanosine | at 80°C | Saccharolobus solfataricus | |
3.2.2.2 | 3.8 | - |
adenosine | - |
Saccharolobus solfataricus | |
3.2.2.2 | 23.3 | - |
Inosine | - |
Saccharolobus solfataricus | |
3.2.2.2 | 29.1 | - |
guanosine | - |
Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.2.1 | Saccharolobus solfataricus | estimated isoelectric point | - |
5.9 |
3.2.2.2 | Saccharolobus solfataricus | estimated | - |
5.92 |