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Literature summary extracted from

  • Alber, B.E.; Kung, J.W.; Fuchs, G.
    3-Hydroxypropionyl-coenzyme A synthetase from Metallosphaera sedula, an enzyme involved in autotrophic CO2 fixation (2008), J. Bacteriol., 190, 1383-1389.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.17 additional information 3-hydroxypropionyl-CoA synthetase is up-regulated eightfold in autotrophically versus heterotrophically grown Metallosphaera sedula Metallosphaera sedula

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.17 expression of the His-tagged enzyme in Escherichia coli Sulfurisphaera tokodaii
6.2.1.36 expression in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.17 additional information
-
 additional information the dependency of the 3-hydroxypropionyl-CoA synthetase with respect to CoA does not follow Michaelis-Menten kinetics, overview Metallosphaera sedula
6.2.1.17 0.045
-
 ATP pH 8.4, 65°C Metallosphaera sedula
6.2.1.17 0.11
-
 ATP pH 8.4, 65°C Sulfurisphaera tokodaii
6.2.1.17 0.12
-
 propanoate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
6.2.1.17 0.18
-
 3-hydroxypropanoate pH 8.4, 65°C Metallosphaera sedula
6.2.1.17 0.19
-
 3-hydroxypropanoate pH 8.4, 65°C Sulfurisphaera tokodaii
6.2.1.17 1.42
-
 Acrylate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
6.2.1.36 0.045
-
 ATP pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 0.12
-
 propionate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 0.18
-
 3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 1.42
-
 Acrylate pH 8.5, 45°C Metallosphaera sedula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.17 Mg2+
-
 Sulfurisphaera tokodaii
6.2.1.17 Mg2+
-
 Metallosphaera sedula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.17 74000
-
 2 * 74000 Sulfurisphaera tokodaii
6.2.1.17 78000
-
 4 * 78000 Metallosphaera sedula
6.2.1.17 140000
-
 recombinant enzyme, gel filtration Sulfurisphaera tokodaii
6.2.1.17 340000
-
 gel filtration Metallosphaera sedula
6.2.1.36 74000
-
 2 * 74000, SDS-PAGE Sulfurisphaera tokodaii
6.2.1.36 78000
-
 4 * 78000, SDS-PAGE Metallosphaera sedula
6.2.1.36 140000
-
 gel filtration Sulfurisphaera tokodaii
6.2.1.36 340000
-
 gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.17 ATP + 3-hydroxypropanoate + CoA Sulfurisphaera tokodaii
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropanoate + CoA Metallosphaera sedula
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropanoate + CoA Metallosphaera sedula DSM 5348
-
 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A Metallosphaera sedula the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A Sulfurisphaera tokodaii the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A Sulfurisphaera tokodaii DSM 16993 the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A Metallosphaera sedula DSM 5348 the enzyme is involved in autotrophic CO2 fixation 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.17 Metallosphaera sedula A4YGR1
-
-
6.2.1.17 Sulfurisphaera tokodaii
-
-
-
6.2.1.36 Metallosphaera sedula A4YGR1
-
-
6.2.1.36 Metallosphaera sedula DSM 5348 A4YGR1
-
-
6.2.1.36 Sulfurisphaera tokodaii Q973W5
-
-
6.2.1.36 Sulfurisphaera tokodaii DSM 16993 Q973W5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.17 native enzyme 95fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, anion exchange and adsorption chromatography, followed by gel filtration Metallosphaera sedula
6.2.1.17 recombinant His-tagged enzyme from Escherichia coli Sulfurisphaera tokodaii
6.2.1.36
-
 Metallosphaera sedula
6.2.1.36 recombinant enzyme Sulfurisphaera tokodaii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.17 additional information autotrophically grown cells Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.17 0.012
-
 heterotrophic grown cells Metallosphaera sedula
6.2.1.17 0.091
-
 autotrophic grown cells Metallosphaera sedula
6.2.1.17 6.7
-
 purified recombinant enzyme Sulfurisphaera tokodaii
6.2.1.17 18
-
 purified native enzyme Metallosphaera sedula
6.2.1.36 6.7
-
 pH 8,5, 65°C Sulfurisphaera tokodaii
6.2.1.36 18
-
 pH 8,5, 45°C, autotrophically grown cells Metallosphaera sedula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.17 ATP + 3-hydroxypropanoate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropanoate + CoA
-
 Metallosphaera sedula AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropanoate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + 3-hydroxypropanoyl-CoA
-
 ?
6.2.1.17 ATP + acetate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA
-
 Metallosphaera sedula AMP + diphosphate + acryloyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + acryloyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + acrylyl-CoA
-
 ?
6.2.1.17 ATP + butyrate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA
-
 Sulfurisphaera tokodaii AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA
-
 Metallosphaera sedula AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA
-
 Metallosphaera sedula DSM 5348 AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.17 additional information the recombinant enzyme not only activates 3-hydroxypropionate to its CoA ester but also activates propionate, acrylate, acetate, and butyrate with reduced substrates affinities compared to propionate and 3-hydroxypropionate Sulfurisphaera tokodaii ?
-
 ?
6.2.1.36 3-chloropropionate + ATP + coenzyme A 64% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii 3-chloropropionyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-chloropropionate + ATP + coenzyme A 64% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii DSM 16993 3-chloropropionyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A
-
 Sulfurisphaera tokodaii 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Sulfurisphaera tokodaii 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is specific for 3-hydroxypropionate and propionate Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A
-
 Sulfurisphaera tokodaii DSM 16993 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Sulfurisphaera tokodaii DSM 16993 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in autotrophic CO2 fixation Metallosphaera sedula DSM 5348 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + CTP + coenzyme A 13% of the activity with ATP Metallosphaera sedula 3-hydroxypropanoyl-CoA + CMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + UTP + coenzyme A 32% of the activity with ATP Metallosphaera sedula 3-hydroxypropanoyl-CoA + UMP + diphosphate
-
 ?
6.2.1.36 3-mercaptopropionate + ATP + coenzyme A 36% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii 3-mercaptopropionyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-mercaptopropionate + ATP + coenzyme A 36% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii DSM 16993 3-mercaptopropionyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 acetate + ATP + coenzyme A 42% of the activity with 3-hydroxypropionate Metallosphaera sedula acetyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 acetate + ATP + coenzyme A 65% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii acetyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 acrylate + ATP + coenzyme A 77% of the activity with 3-hydroxypropionate Metallosphaera sedula acryloyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 acrylate + ATP + coenzyme A 96% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii acryloyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 butyrate + ATP + coenzyme A 20% of the activity with 3-hydroxypropionate Metallosphaera sedula butyryl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 butyrate + ATP + coenzyme A 27% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii butyryl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Metallosphaera sedula ?
-
 ?
6.2.1.36 additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Sulfurisphaera tokodaii ?
-
 ?
6.2.1.36 additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Sulfurisphaera tokodaii DSM 16993 ?
-
 ?
6.2.1.36 additional information less than 1% of the activity with 3-hydroxypropionate: 3-hydroxybutyrate, crotonate, glycerate, malonate Metallosphaera sedula DSM 5348 ?
-
 ?
6.2.1.36 propionate + ATP + coenzyme A 98% of the activity with 3-hydroxypropionate Sulfurisphaera tokodaii propionyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 propionate + ATP + coenzyme A the enzyme is specific for 3-hydroxypropionate and propionate Metallosphaera sedula propionyl-CoA + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.17 dimer 2 * 74000 Sulfurisphaera tokodaii
6.2.1.17 tetramer 4 * 78000 Metallosphaera sedula
6.2.1.36 dimer 2 * 74000, SDS-PAGE Sulfurisphaera tokodaii
6.2.1.36 homotetramer 4 * 78000, SDS-PAGE Metallosphaera sedula

Synonyms

EC Number Synonyms Comment Organism
6.2.1.17 3-hydroxypropionyl-CoA synthetase
-
 Sulfurisphaera tokodaii
6.2.1.17 3-hydroxypropionyl-CoA synthetase
-
 Metallosphaera sedula
6.2.1.17 3-hydroxypropionyl-coenzyme A synthetase
-
 Sulfurisphaera tokodaii
6.2.1.17 3-hydroxypropionyl-coenzyme A synthetase
-
 Metallosphaera sedula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.17 65
-
 assay at Sulfurisphaera tokodaii
6.2.1.17 65
-
 assay at Metallosphaera sedula
6.2.1.36 45
-
 assay at Metallosphaera sedula
6.2.1.36 65
-
 assay at Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.36 80
-
 15 min, stable up to 80°C Metallosphaera sedula
6.2.1.36 100
-
 15 min, 50% loss of activity Metallosphaera sedula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.17 5.4
-
 Acrylate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
6.2.1.17 5.6
-
 propanoate pH 8.4, 45°C, coupled spectrophotometric assay Metallosphaera sedula
6.2.1.17 8
-
 3-hydroxypropanoate pH 8.4, 65°C Sulfurisphaera tokodaii
6.2.1.17 23
-
 3-hydroxypropanoate pH 8.4, 65°C Metallosphaera sedula
6.2.1.36 5.4
-
 Acrylate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 5.6
-
 propionate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 5.7
-
 3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.17 8.4
-
 assay at Sulfurisphaera tokodaii
6.2.1.17 8.4
-
 assay at Metallosphaera sedula
6.2.1.36 8.5
-
 assay at Metallosphaera sedula
6.2.1.36 8.5
-
 assay at Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.17 ATP
-
 Sulfurisphaera tokodaii
6.2.1.17 ATP
-
 Metallosphaera sedula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.36 3.8
-
 Acrylate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 31.7
-
 3-hydroxypropionate pH 8.5, 45°C Metallosphaera sedula
6.2.1.36 46.7
-
 propionate pH 8.5, 45°C Metallosphaera sedula