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Literature summary extracted from

  • Rothacker, B.; Werr, M.; Ilg, T.
    Molecular cloning, partial genomic structure and functional characterization of succinic semialdehyde dehydrogenase genes from the parasitic insects Lucilia cuprina and Ctenocephalides felis (2008), Insect Mol. Biol., 17, 279-291.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.24 additional information genomic copy of the SSADH gene contains two introns. Multiple SSADH gene copies are present in the genome Lucilia cuprina
1.2.1.24 additional information genomic copy of the SSADH gene is devoid of introns. Multiple SSADH gene copies are present in the genome Ctenocephalides felis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.24 into vectors pQE30 and into pMalc2x. Expression in Escherichia coli strains M15 and TB1 Ctenocephalides felis
1.2.1.24 into vectors pQE30 and into pMalc2x. Expression in Escherichia coli strains M15 and TB1 Lucilia cuprina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.24 succinate semialdehyde inhibition by the succinate semialdehyde substrate is starting already at a concentration as low as 0.02 mM Ctenocephalides felis
1.2.1.24 succinate semialdehyde inhibition by the succinate semialdehyde substrate is starting already at a concentration as low as 0.02 mM Lucilia cuprina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 0.001
-
 succinate semialdehyde
-
 Ctenocephalides felis
1.2.1.24 0.0027
-
 succinate semialdehyde
-
 Lucilia cuprina
1.2.1.24 0.0121
-
 4-carboxybenzaldehyde
-
 Ctenocephalides felis
1.2.1.24 0.017
-
 3-carboxybenzaldehyde
-
 Ctenocephalides felis
1.2.1.24 0.025
-
 pentanal
-
 Ctenocephalides felis
1.2.1.24 0.076
-
 NAD+
-
 Ctenocephalides felis
1.2.1.24 0.0776
-
 4-carboxybenzaldehyde
-
 Lucilia cuprina
1.2.1.24 0.0827
-
 pentanal
-
 Lucilia cuprina
1.2.1.24 0.105
-
 Butanal
-
 Ctenocephalides felis
1.2.1.24 0.112
-
 3-carboxybenzaldehyde
-
 Lucilia cuprina
1.2.1.24 0.241
-
 benzaldehyde
-
 Ctenocephalides felis
1.2.1.24 0.348
-
 NAD+
-
 Lucilia cuprina
1.2.1.24 0.552
-
 NADP+
-
 Lucilia cuprina
1.2.1.24 1.606
-
 propanal
-
 Ctenocephalides felis
1.2.1.24 4.076
-
 acetaldehyde
-
 Ctenocephalides felis
1.2.1.24 4.4
-
 NADP+
-
 Ctenocephalides felis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 52000
-
 deduced protein without the mitochondria import sequence Lucilia cuprina
1.2.1.24 52100
-
 deduced protein without the mitochondria import sequence Ctenocephalides felis
1.2.1.24 55400
-
 deduced protein with the mitochondria import sequence Ctenocephalides felis
1.2.1.24 55400
-
 deduced protein with the mitochondria import sequence Lucilia cuprina

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Ctenocephalides felis
-
-
-
1.2.1.24 Lucilia cuprina B0JFD4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 3-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis 3-carboxybenzoate + NAD(P)H + H+
-
 ?
1.2.1.24 3-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Lucilia cuprina 3-carboxybenzoate + NAD(P)H + H+
-
 ?
1.2.1.24 4-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis 4-carboxybenzoate + NAD(P)H + H+
-
 ?
1.2.1.24 4-carboxybenzaldehyde + NAD(P)+ + H2O
-
 Lucilia cuprina 4-carboxybenzoate + NAD(P)H + H+
-
 ?
1.2.1.24 acetaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis acetate + NAD(P)H + H+
-
 ?
1.2.1.24 benzaldehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis benzoate + NAD(P)H + H+
-
 ?
1.2.1.24 butanal + NAD(P)+ + H2O
-
 Ctenocephalides felis butanoate + NAD(P)H + H+
-
 ?
1.2.1.24 pentanal + NAD(P)+ + H2O
-
 Ctenocephalides felis pentanoate + NAD(P)H + H+
-
 ?
1.2.1.24 pentanal + NAD(P)+ + H2O
-
 Lucilia cuprina pentanoate + NAD(P)H + H+
-
 ?
1.2.1.24 propanal + NAD(P)+ + H2O
-
 Ctenocephalides felis propanoate + NAD(P)H + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD(P)+ + H2O
-
 Ctenocephalides felis succinate + NAD(P)H + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD(P)+ + H2O
-
 Lucilia cuprina succinate + NAD(P)H + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Ctenocephalides felis succinate + NADH + H+
-
 r
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Lucilia cuprina succinate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.24 SSADH
-
 Ctenocephalides felis
1.2.1.24 SSADH
-
 Lucilia cuprina
1.2.1.24 succinic semialdehyde dehydrogenase
-
 Ctenocephalides felis
1.2.1.24 succinic semialdehyde dehydrogenase
-
 Lucilia cuprina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.24 8
-
-
 Lucilia cuprina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.24 8 9
-
 Ctenocephalides felis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.24 NAD+
-
 Ctenocephalides felis
1.2.1.24 NAD+
-
 Lucilia cuprina
1.2.1.24 NADP+
-
 Ctenocephalides felis
1.2.1.24 NADP+
-
 Lucilia cuprina