EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | MK801 | N-methyl-D-aspartate-type glutamate receptor antagonist, activates the enzyme and inhibits osteogenic differentiation of the rat bone marrow stem cells | Homo sapiens | |
6.3.1.2 | additional information | glucocorticoids induce the enzyme transcription and translation in osteoblasts | Homo sapiens | |
6.3.1.2 | additional information | glucocorticoids induce the enzyme transcription and translation in osteoblasts, cortisol and two synthetic compounds dexamethasone and prednisolone highly stimulate enzyme expression. No effects by aldosterone, estradiol, progesterone, dihydrotestosterone, or triiodothyronine. Treatment of MG-63 cells with MG-132, an inhibitor of proteasomal degradation, at 0.02 mM blocks the dexamethasone-induction of glutamine synthetase activity almost completely | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.2 | expression analysis | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | methionine sulfoximine | an irreversible, competitive inhibitor of glutamine synthetase activity | Homo sapiens | |
6.3.1.2 | additional information | the canonical Wnt signalling pathway is a negative regulator of glutamine synthetase activity; the canonical Wnt signalling pathway is a negative regulator of glutamine synthetase activity. No effects by aldosterone, estradiol, progesterone, dihydrotestosterone, or triiodothyronine | Homo sapiens | |
6.3.1.2 | vitamin D | 22% inhibition at 0.00001 mM, reduces dexamethasone induced increase in enzyme expression | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Mg2+ | - |
Homo sapiens | |
6.3.1.2 | Mn2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-glutamate + NH3 | Homo sapiens | - |
ADP + phosphate + L-glutamine | - |
? | |
6.3.1.2 | additional information | Homo sapiens | Wnt and steroid pathways control glutamate signalling by regulating glutamine synthetase activity in osteoblastic cells, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.1.2 | bone marrow stem cell | - |
Homo sapiens | - |
6.3.1.2 | MG-63 cell | osteoblast-like cells | Homo sapiens | - |
6.3.1.2 | additional information | the enzyme is ubiquitously expressed in human tissues | Homo sapiens | - |
6.3.1.2 | osteoblast | - |
Homo sapiens | - |
6.3.1.2 | osteoblast | primary derived from bone marrow | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 0.018 | - |
glutamine synthetase activity in MG-63 cells | Homo sapiens |
6.3.1.2 | 0.022 | - |
glutamine synthetase activity in MG-63 cells in presence of MG-132 | Homo sapiens |
6.3.1.2 | 0.023 | - |
glutamine synthetase activity in MG-63 cells in presence of dexamethasone and MG-132 | Homo sapiens |
6.3.1.2 | 0.042 | - |
glutamine synthetase activity in MG-63 cells in presence of vitamin D | Homo sapiens |
6.3.1.2 | 0.067 | - |
glutamine synthetase activity in MG-63 cells in presence of dexamethasone | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-glutamate + NH3 | - |
Homo sapiens | ADP + phosphate + L-glutamine | - |
? | |
6.3.1.2 | additional information | Wnt and steroid pathways control glutamate signalling by regulating glutamine synthetase activity in osteoblastic cells, overview | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.2 | Glutamine synthetase | - |
Homo sapiens |
6.3.1.2 | L-glutamate:ammonia ligase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 6.8 | - |
assay at | Homo sapiens |