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Literature summary extracted from

  • Jaeger, M.; Rothacker, B.; Ilg, T.
    Saturation transfer difference NMR studies on substrates and inhibitors of succinic semialdehyde dehydrogenases (2008), Biochem. Biophys. Res. Commun., 372, 400-406.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16
-
Drosophila melanogaster
1.2.1.79
-
Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.16 C311A mutant is still soluble but unable to catalyze succinate semialdehyde oxidation. Mutation leads to an inactive product binding both succinate semialdehyde aldehyde and gem-diol Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 3-tolualdehyde only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme Drosophila melanogaster
1.2.1.79 3-tolualdehyde only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.79 0.0078
-
succinate semialdehyde pH 8.5, 22°C Escherichia coli
1.2.1.79 0.0446
-
NADP+ pH 8.5, 22°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Drosophila melanogaster
-
-
-
1.2.1.79 Escherichia coli P25526
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.79 34
-
pH 8.5, 22°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 additional information only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme. Residue cysteine311 is crucial for their discrimination Drosophila melanogaster ?
-
?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
Drosophila melanogaster succinate + NADPH + 2 H+
-
?
1.2.1.79 3-carboxybenzaldehyde + NADP+ + H2O
-
Escherichia coli 3-carboxybenzoate + NADPH + 2 H+
-
?
1.2.1.79 3-nitrobenzaldehyde + NADP+ + H2O
-
Escherichia coli 3-nitrobenzoate + NADPH + 2 H+
-
?
1.2.1.79 4-carboxybenzaldehyde + NADP+ + H2O
-
Escherichia coli 4-carboxybenzoate + NADPH + H+
-
?
1.2.1.79 benzaldehyde + NADP+ + H2O
-
Escherichia coli benzoate + NADPH + 2 H+
-
?
1.2.1.79 additional information only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme Escherichia coli ?
-
?
1.2.1.79 n-butanal + NADP+ + H2O
-
Escherichia coli butanoate + NADPH + 2 H+
-
?
1.2.1.79 n-hexanal + NADP+ + H2O
-
Escherichia coli hexanoate + NADPH + H+
-
?
1.2.1.79 n-pentanal + NADP+ + H2O
-
Escherichia coli n-pentanoate + NADPH + H+
-
?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
Escherichia coli succinate + NADPH + 2 H+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.79 22
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.79 8.5
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.79 7.4
-
75% of maximum activity Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ higher activity in the presence of NAD+ than in the presence of NADP+ Drosophila melanogaster
1.2.1.16 NADP+ higher activity in the presence of NAD+ than in the presence of NADP+ Drosophila melanogaster
1.2.1.79 NAD+ NAD+ acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ Escherichia coli
1.2.1.79 NADP+ NAD+ also acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.79 0.08
-
pH 8.5, 22°C Escherichia coli 3-tolualdehyde