EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.16 | - |
Drosophila melanogaster |
1.2.1.79 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.16 | C311A | mutant is still soluble but unable to catalyze succinate semialdehyde oxidation. Mutation leads to an inactive product binding both succinate semialdehyde aldehyde and gem-diol | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | 3-tolualdehyde | only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme | Drosophila melanogaster | |
1.2.1.79 | 3-tolualdehyde | only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.79 | 0.0078 | - |
succinate semialdehyde | pH 8.5, 22°C | Escherichia coli | |
1.2.1.79 | 0.0446 | - |
NADP+ | pH 8.5, 22°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.16 | Drosophila melanogaster | - |
- |
- |
1.2.1.79 | Escherichia coli | P25526 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.79 | 34 | - |
pH 8.5, 22°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | additional information | only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme. Residue cysteine311 is crucial for their discrimination | Drosophila melanogaster | ? | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Drosophila melanogaster | succinate + NADPH + 2 H+ | - |
? | |
1.2.1.79 | 3-carboxybenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 3-carboxybenzoate + NADPH + 2 H+ | - |
? | |
1.2.1.79 | 3-nitrobenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 3-nitrobenzoate + NADPH + 2 H+ | - |
? | |
1.2.1.79 | 4-carboxybenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 4-carboxybenzoate + NADPH + H+ | - |
? | |
1.2.1.79 | benzaldehyde + NADP+ + H2O | - |
Escherichia coli | benzoate + NADPH + 2 H+ | - |
? | |
1.2.1.79 | additional information | only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme | Escherichia coli | ? | - |
? | |
1.2.1.79 | n-butanal + NADP+ + H2O | - |
Escherichia coli | butanoate + NADPH + 2 H+ | - |
? | |
1.2.1.79 | n-hexanal + NADP+ + H2O | - |
Escherichia coli | hexanoate + NADPH + H+ | - |
? | |
1.2.1.79 | n-pentanal + NADP+ + H2O | - |
Escherichia coli | n-pentanoate + NADPH + H+ | - |
? | |
1.2.1.79 | succinate semialdehyde + NADP+ + H2O | - |
Escherichia coli | succinate + NADPH + 2 H+ | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.79 | 22 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.79 | 8.5 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.79 | 7.4 | - |
75% of maximum activity | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | NAD+ | higher activity in the presence of NAD+ than in the presence of NADP+ | Drosophila melanogaster | |
1.2.1.16 | NADP+ | higher activity in the presence of NAD+ than in the presence of NADP+ | Drosophila melanogaster | |
1.2.1.79 | NAD+ | NAD+ acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ | Escherichia coli | |
1.2.1.79 | NADP+ | NAD+ also acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ | Escherichia coli |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.2.1.79 | 0.08 | - |
pH 8.5, 22°C | Escherichia coli | 3-tolualdehyde |