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Literature summary extracted from

  • Beisswenger, R.; Kula, M.R.
    Catalytic properties and substrate specificity of 3-hexulose phosphate synthase from Methylomonas M15 (1991), Appl. Microbiol. Biotechnol., 34, 604-607.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 EDTA complete inhibition at 10 mM Methylomonas sp.
4.1.2.43 additional information not influenced by NaBH4 Methylomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Mg2+ required for activity Methylomonas sp.
4.1.2.43 Mn2+ required for activity Methylomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 22000
-
 SDS-PAGE Methylomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde Methylomonas sp.
-
 D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde Methylomonas sp. M15 / DSM 580
-
 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Methylomonas sp.
-
-
-
4.1.2.43 Methylomonas sp. M15 / DSM 580
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 polyethylene imine precipitation, S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephadex G-75 gel filtration Methylomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 26
-
 crude extract Methylomonas sp.
4.1.2.43 4100
-
 after 161fold purification Methylomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + acetaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + acetaldehyde
-
 Methylomonas sp. M15 / DSM 580 ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + benzaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + benzaldehyde
-
 Methylomonas sp. M15 / DSM 580 ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + bromoacetaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + bromoacetaldehyde
-
 Methylomonas sp. M15 / DSM 580 ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + butyraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + chloral
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + chloroacetaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + chloropropionaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + dichloroacetaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + DL-glyceraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. M15 / DSM 580 D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + glutaraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + glycolaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + glyoxal
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + isobutyraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + isovaleraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + methylglyoxal
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + methylmercaptopropionaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + propionaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + pyridine-2-carboxaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + pyridine-3-carboxaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + pyridine-4-carboxaldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + valeraldehyde
-
 Methylomonas sp. ?
-
 ?
4.1.2.43 additional information does not accept D-ribose 5-phosphate, D-ribulose, D-xylose, D-xylulose 5-phosphate, D-fructose 6-phosphate, D-fructose 1-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-glucose, D,L-glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, hydroxypyruvate, dihydroxyacetone, D-erythrose 4-phospate, pivalaldehyde, acrolein, crotonaldehyde, glyoxalic acid, malonaldehyde, furfural as substrates Methylomonas sp. ?
-
 ?
4.1.2.43 additional information does not accept D-ribose 5-phosphate, D-ribulose, D-xylose, D-xylulose 5-phosphate, D-fructose 6-phosphate, D-fructose 1-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose, D-glucose, D,L-glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, hydroxypyruvate, dihydroxyacetone, D-erythrose 4-phospate, pivalaldehyde, acrolein, crotonaldehyde, glyoxalic acid, malonaldehyde, furfural as substrates Methylomonas sp. M15 / DSM 580 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose phosphate synthase
-
 Methylomonas sp.
4.1.2.43 HPS-aldolase
-
 Methylomonas sp.