EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.21 | 4-aminoantipirine | 0.009%, ranging from 0.006 to 0.074% | Saccharomyces cerevisiae | |
1.1.3.21 | phenol | activity positively affected by the presence of 0.018% phenol, ranging from 0.006 to 0.123% | Saccharomyces cerevisiae | |
1.1.3.21 | Triton X-100 | 0.1% of Triton X-100 slightly stimulates enzyme activity | Saccharomyces cerevisiae |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.30 | synthesis | methodology of dosage of glycerol kinase by response surface modeling of the enzymatic reaction. This low cost method for glycerol kinase dosage in a sequence of reactions is of great importance for many industries, like food, sugar and alcohol. Response surface modeling shows to be an adequate approach for modeling the reaction and optimization of reaction conditions to maximize glycerol kinase activity | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.30 | 2 | - |
glycerol | - |
Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.21 | Saccharomyces cerevisiae | - |
- |
- |
2.7.1.30 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | additional information | - |
consumption of oxygen determined by oxygraphy, activity measured by peroxidase chromogen method, a 120 min reaction time and a concentration of 0.5 M of glycerol phosphate considered as best conditions for determination of enzyme activity | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.3.21 | 40°C, during storage for 21 days 20% of activity lost, sodium azide as stabilizer in a concentration of 0.05% maintains activity of the enzyme in 98.4%, addition of sucrose maintains only 74% of activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.21 | sn-glycerol-3-phosphate + O2 | - |
Saccharomyces cerevisiae | glycerone phosphate + H2O2 | - |
? | |
2.7.1.30 | ATP + glycerol | - |
Saccharomyces cerevisiae | ADP + sn-glycerol 3-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 60 | - |
at pH 8.0, tested at temperatures of 25, 30, 37, 45, 50, 55, 60, 65, 70°C | Saccharomyces cerevisiae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 30 | 80 | activity measured after incubating the enzyme in Tris/HCl buffer at various temperatures for 1 h | Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 30 | 80 | activity measured after incubating the enzyme solution for 1 h at various temperatures of 0, 30, 50, 60, 70, 80°C in Tris/HCl buffer, activity of 0.222 U/ml at 0°C and of 0.238 U/ml at 80°C | Saccharomyces cerevisiae |
2.7.1.30 | 50 | - |
1 h, stable | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 8.5 | - |
assayed between pH 5.0 and pH 10.0 | Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 5 | 10 | activity within, optimum at pH 8.5 | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | 8 | - |
lower activity above pH 9.0, tested in a range between pH 6-10 | Saccharomyces cerevisiae |
2.7.1.30 | 6 | 8 | high stability | Saccharomyces cerevisiae |