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Literature summary extracted from

  • Aragon Casale, C.; Ferreira-Dias, S.; Gattas Aparecida de Lucca, E.; Maristela de Freitas Sanches, P.
    Characterization of glycerol kinase from bakers yeast: Response surface modeling of the enzymatic reaction (2008), J. Mol. Catal. B, 52-53, 113-120.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.21 4-aminoantipirine 0.009%, ranging from 0.006 to 0.074% Saccharomyces cerevisiae
1.1.3.21 phenol activity positively affected by the presence of 0.018% phenol, ranging from 0.006 to 0.123% Saccharomyces cerevisiae
1.1.3.21 Triton X-100 0.1% of Triton X-100 slightly stimulates enzyme activity Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
2.7.1.30 synthesis methodology of dosage of glycerol kinase by response surface modeling of the enzymatic reaction. This low cost method for glycerol kinase dosage in a sequence of reactions is of great importance for many industries, like food, sugar and alcohol. Response surface modeling shows to be an adequate approach for modeling the reaction and optimization of reaction conditions to maximize glycerol kinase activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 2
-
 glycerol
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.21 Saccharomyces cerevisiae
-
-
-
2.7.1.30 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.21 additional information
-
 consumption of oxygen determined by oxygraphy, activity measured by peroxidase chromogen method, a 120 min reaction time and a concentration of 0.5 M of glycerol phosphate considered as best conditions for determination of enzyme activity Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.3.21 40°C, during storage for 21 days 20% of activity lost, sodium azide as stabilizer in a concentration of 0.05% maintains activity of the enzyme in 98.4%, addition of sucrose maintains only 74% of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.21 sn-glycerol-3-phosphate + O2
-
 Saccharomyces cerevisiae glycerone phosphate + H2O2
-
 ?
2.7.1.30 ATP + glycerol
-
 Saccharomyces cerevisiae ADP + sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.21 60
-
 at pH 8.0, tested at temperatures of 25, 30, 37, 45, 50, 55, 60, 65, 70°C Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.21 30 80 activity measured after incubating the enzyme in Tris/HCl buffer at various temperatures for 1 h Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.21 30 80 activity measured after incubating the enzyme solution for 1 h at various temperatures of 0, 30, 50, 60, 70, 80°C in Tris/HCl buffer, activity of 0.222 U/ml at 0°C and of 0.238 U/ml at 80°C Saccharomyces cerevisiae
2.7.1.30 50
-
 1 h, stable Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.21 8.5
-
 assayed between pH 5.0 and pH 10.0 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.21 5 10 activity within, optimum at pH 8.5 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.21 8
-
 lower activity above pH 9.0, tested in a range between pH 6-10 Saccharomyces cerevisiae
2.7.1.30 6 8 high stability Saccharomyces cerevisiae