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Literature summary extracted from

  • Morita, S.Y.; Kobayashi, A.; Takanezawa, Y.; Kioka, N.; Handa, T.; Arai, H.; Matsuo, M.; Ueda, K.
    Bile salt-dependent efflux of cellular phospholipids mediated by ATP binding cassette protein B4 (2007), Hepatology, 46, 188-199.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.1 cholate slightly increases the efflux of phospholipids and cholesterol from cells Homo sapiens
7.6.2.1 glycocholate slightly increases the efflux of phospholipids and cholesterol from cells Homo sapiens
7.6.2.1 additional information effects of bile salt structure on ABCB4-mediated lipid secretion, overview Homo sapiens
7.6.2.1 taurocholate increases the efflux of phospholipids and cholesterol from cells, the taurocholate monomer plays an important role in ABCB4-mediated lipid secretion Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.1 stable expression of ABCB4 in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.1 K1075M site-directed mutagenesis in the Walker A motif results in an inactive mutant Homo sapiens
7.6.2.1 K435M site-directed mutagenesis in the Walker A motif results in an inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.1 verapamil complete inhibition at 0.05 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.1 plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.1 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.1 140000
-
 x * 140000, recombinant ABCB4, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.1 ATP + H2O + phosphatidylserine/in Homo sapiens ABCB4 mediates the efflux of phospholipids into the canalicular lumen in the presence of bile salts, and plays a crucial role in bile formation and lipid homeostasis ADP + phosphate + phosphatidylserine/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.6.2.1 glycoprotein analysis of glycosylation of ABCB4, cleavage by endoglycosidase H or peptide N-glycosidase F, overview Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.6.2.1 hepatocyte canalicular membrane Homo sapiens
-
7.6.2.1 additional information no endogenous ABCB4 activity in HEK-293 cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.1 ATP + H2O + phosphatidylserine/in ABCB4 mediates the efflux of phospholipids into the canalicular lumen in the presence of bile salts, and plays a crucial role in bile formation and lipid homeostasis Homo sapiens ADP + phosphate + phosphatidylserine/out
-
 ?
7.6.2.1 ATP + H2O + phosphatidylserine/in ABCB4 is required for ATP-dependent phosphatidylcholine secretion into the bile and to translocate phosphatidylcholine across the plasma membrane, overview Homo sapiens ADP + phosphate + phosphatidylserine/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.1 ? x * 140000, recombinant ABCB4, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
7.6.2.1 ABCB4
-
 Homo sapiens
7.6.2.1 MDR3 P-glycoprotein
-
 Homo sapiens
7.6.2.1 More cf. EC 3.6.3.44 Homo sapiens
7.6.2.1 multidrug resistance 3 P-glycoprotein
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.6.2.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6.2.1 7.4
-
 assay at Homo sapiens