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Literature summary extracted from

  • Li, X.; Jiang, X.; Li, H.; Ren, D.
    Purine nucleoside phosphorylase from Pseudoalteromonas sp. Bsi590: molecular cloning, gene expression and characterization of the recombinant protein (2008), Extremophiles, 12, 325-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expressed in Escherichia coli Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.21
-
phosphate
-
Pseudoalteromonas sp.
2.4.2.1 0.32
-
Inosine 37°C Escherichia coli
2.4.2.1 0.34
-
phosphate 37°C Escherichia coli
2.4.2.1 0.67
-
Inosine
-
Pseudoalteromonas sp.
2.4.2.1 0.83
-
Inosine 50°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 25018
-
x * 25018, calculated from sequence Pseudoalteromonas sp.
2.4.2.1 29000
-
x * 29000, SDS-PAGE Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Escherichia coli
-
-
-
2.4.2.1 Pseudoalteromonas sp. A3F8V1 Bsi590
-
2.4.2.1 Pseudoalteromonas sp. Bsi590 A3F8V1 Bsi590
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxy-6-methylpurine nucleoside + phosphate 13.2% of the activity with inosine Pseudoalteromonas sp. ?
-
?
2.4.2.1 2'-deoxy-6-methylpurine nucleoside + phosphate 13.2% of the activity with inosine Pseudoalteromonas sp. Bsi590 ?
-
?
2.4.2.1 2'-deoxyadenosine + phosphate 13.4% of the activity with inosine Pseudoalteromonas sp. adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2.4.2.1 2'-deoxyguanosine + phosphate 3.7% of the activity with inosine Pseudoalteromonas sp. guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2.4.2.1 2'-deoxyguanosine + phosphate 3.7% of the activity with inosine Pseudoalteromonas sp. Bsi590 guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2.4.2.1 adenosine + phosphate 15.4% of the activity with inosine Pseudoalteromonas sp. adenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 adenosine + phosphate 15.4% of the activity with inosine Pseudoalteromonas sp. Bsi590 adenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 arabinofuranosyladenine + phosphate 4.3% of the activity with inosine Pseudoalteromonas sp. ?
-
?
2.4.2.1 guanosine + phosphate 55.8% of the activity with inosine Pseudoalteromonas sp. guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 guanosine + phosphate 55.8% of the activity with inosine Pseudoalteromonas sp. Bsi590 guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Pseudoalteromonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Pseudoalteromonas sp. Bsi590 hypoxanthine + alpha-D-ribose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 ? x * 29000, SDS-PAGE Pseudoalteromonas sp.
2.4.2.1 ? x * 25018, calculated from sequence Pseudoalteromonas sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 EcPNP
-
Escherichia coli
2.4.2.1 PiPNP
-
Pseudoalteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 30 35
-
Pseudoalteromonas sp.
2.4.2.1 60
-
-
Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.1 15 50 15°C: about 45% of maximal activity, 50°C: about 45% of maximal activity Pseudoalteromonas sp.
2.4.2.1 25 70 25°C: about 40% of maximal activity, 70°C: about 60% of maximal activity Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 37
-
30 min, stable up to Pseudoalteromonas sp.
2.4.2.1 42
-
30 min, 26% loss of activity Pseudoalteromonas sp.
2.4.2.1 50
-
30 min, 15% loss of activity Escherichia coli
2.4.2.1 50
-
after 1 h of incubation at 50°C with 0.5 M KCl and 1 M KCl, the enzyme activities still retaines 81% and 38%, respectively. It is inactivated without KCl Pseudoalteromonas sp.
2.4.2.1 65
-
30 min, complete inactivation Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 44.93
-
phosphate 37°C Escherichia coli
2.4.2.1 44.93
-
Inosine 37°C Escherichia coli
2.4.2.1 104.9
-
phosphate 50°C Escherichia coli
2.4.2.1 104.9
-
Inosine 50°C Escherichia coli
2.4.2.1 113.6
-
phosphate
-
Pseudoalteromonas sp.
2.4.2.1 113.6
-
Inosine
-
Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 8 10
-
Pseudoalteromonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.1 10
-
stable for 2.5 h Pseudoalteromonas sp.