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Literature summary extracted from

  • Ali, E.M.
    Fasciola gigantica: purification and characterization of adenosine deaminase (2008), Exp. Parasitol., 119, 285-290.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.4 Ba2+ 44% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Ca2+ 69% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Cu2+ 81% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Hg2+ 88% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Mn2+ 85% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Ni2+ 61% inhibition at 5 mM Fasciola gigantica
3.5.4.4 Zn2+ 65% inhibition at 5 mM Fasciola gigantica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.4 1
-
 adenosine pH 7.2, 37°C, ADAII Fasciola gigantica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.4 Mg2+ activates Fasciola gigantica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.4 29000
-
 x * 29000, ADAII, SDS-PAGE Fasciola gigantica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.4 adenosine + H2O Fasciola gigantica
-
 inosine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.4 Fasciola gigantica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.4 native ADAI 12.3fold by acetone precipitation and ion exchange chromatography, native ADAII 176.6fold by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity Fasciola gigantica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.4 additional information
-
 75.7 U/g tissue, ADAII Fasciola gigantica
3.5.4.4 4.4
-
 partially purified ADAI Fasciola gigantica
3.5.4.4 62.7
-
 purified ADAII Fasciola gigantica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.4 2'-deoxyadenosine + H2O ADAII shows 88% of the activity with adenosine Fasciola gigantica 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyguanosine + H2O ADAII shows 41% of the activity with adenosine Fasciola gigantica 2'-deoxyxanthosine + NH3
-
 ?
3.5.4.4 adenine + H2O ADAII shows 70% of the activity with adenosine Fasciola gigantica hypoxanthine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Fasciola gigantica inosine + NH3
-
 ?
3.5.4.4 adenosine + H2O preferred substrate Fasciola gigantica inosine + NH3
-
 ?
3.5.4.4 guanine + H2O ADAII shows 47% of the activity with adenosine Fasciola gigantica xanthine + NH3
-
 ?
3.5.4.4 guanosine + H2O ADAII shows 75% of the activity with adenosine Fasciola gigantica xanthosine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.4 ? x * 29000, ADAII, SDS-PAGE Fasciola gigantica

Synonyms

EC Number Synonyms Comment Organism
3.5.4.4 ADA
-
 Fasciola gigantica
3.5.4.4 ADAI
-
 Fasciola gigantica
3.5.4.4 ADAII
-
 Fasciola gigantica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.4 40
-
 ADAII Fasciola gigantica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.4.4 10 80 activity range of ADAII Fasciola gigantica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.4 40
-
 ADAII, stable up to Fasciola gigantica
3.5.4.4 90
-
 loss of 95% activity, ADAII Fasciola gigantica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.4 7.5
-
 ADAII Fasciola gigantica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.4 5 9.5 ADAII shows 7% of maximal activity at pH 5.5 and 86% at pH 9.0 Fasciola gigantica