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Literature summary extracted from

  • Kim, D.Y.; Rha, E.; Choi, S.L.; Song, J.J.; Hong, S.P.; Sung, M.H.; Lee, S.G.
    Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase (2007), J. Microbiol. Biotechnol., 17, 116-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.2 expressed in Escherichia coli strain BL21 Symbiobacterium toebii

General Stability

EC Number General Stability Organism
4.1.99.2 the enzyme maintains its original activity at phenol concentrations below 75 mM Symbiobacterium toebii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.2 phenol 100 mM and above Symbiobacterium toebii

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Symbiobacterium toebii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.2 partial purification by methanol treatment Symbiobacterium toebii

Storage Stability

EC Number Storage Stability Organism
4.1.99.2 40°C, in the presence of 75 mM phenol at pH 8.5, 18h, no loss of activity Symbiobacterium toebii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 L-tyrosine + H2O
-
 Symbiobacterium toebii phenol + pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.2 L-tyrosine phenol-lyase
-
 Symbiobacterium toebii
4.1.99.2 TPL
-
 Symbiobacterium toebii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.99.2 6 9.5
-
 Symbiobacterium toebii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.2 pyridoxal 5'-phosphate
-
 Symbiobacterium toebii