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Literature summary extracted from

  • Liu, J.; Zhang, X.; Zhou, S.; Tao, P.; Liu, J.
    Purification and characterization of a 4-hydroxybenzoate decarboxylase from Chlamydophila pneumoniae AR39 (2007), Curr. Microbiol., 54, 102-107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.61 expressed in Escherichia coli strain BL21 (DE3) Chlamydia pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.61 0.21
-
 4-hydroxybenzoate in 20 mM Tris-HCl (pH 7.5) containing 1 mM Mg2+, at 30°C Chlamydia pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.61 Ca2+ 1 mM, 1.44fold stimulation of activity Chlamydia pneumoniae
4.1.1.61 Fe2+ 1 mM, 2.52fold stimulation of activity Chlamydia pneumoniae
4.1.1.61 Mg2+ 1 mM, 2.99fold stimulation of activity Chlamydia pneumoniae
4.1.1.61 Mn2+ 1 mM, 2.43fold stimulation of activity Chlamydia pneumoniae
4.1.1.61 additional information not stimulated by Cu2+ or Zn2+ Chlamydia pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.61 64000
-
 SDS-PAGE Chlamydia pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.61 Chlamydia pneumoniae
-
-
-
4.1.1.61 Chlamydia pneumoniae AR39
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.61 under anaerobic conditions (oxygen-free N2) at 4°C by affinity chromatography Chlamydia pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.61 0.00025
-
 crude cell extract, at 30°C Chlamydia pneumoniae
4.1.1.61 0.00506
-
 after purification, at 30°C Chlamydia pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.61 4-Hydroxybenzoate
-
 Chlamydia pneumoniae Phenol + CO2
-
 r
4.1.1.61 4-Hydroxybenzoate
-
 Chlamydia pneumoniae AR39 Phenol + CO2
-
 r
4.1.1.61 Phenol + CO2 weak reaction Chlamydia pneumoniae 4-Hydroxybenzoate
-
 r
4.1.1.61 Phenol + CO2 weak reaction Chlamydia pneumoniae AR39 4-Hydroxybenzoate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.1.61 HBDC
-
 Chlamydia pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.61 30
-
-
 Chlamydia pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.61 40 50 loses more than 50% decarboxylase activity at 40°C for 5 min, and less than 25% enzyme activity remains after incubation at 50°C for 5 min Chlamydia pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.61 7.5
-
-
 Chlamydia pneumoniae