EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.9 | additional information | a few changes in key residues are sufficient to convert an OMT into a S-methyltransferase | Catharanthus roseus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.9 | subcloned for expression of a His-tagged recombinant protein in Escherichia coli | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.9 | additional information | not inhibited by Mg2+ | Catharanthus roseus | |
2.1.1.9 | Zn2+ | ca. 90% inhibition at 1 mM, at low concentrations the effect is substrate-dependent | Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.9 | 0.056 | - |
furfuryl thiol | - |
Catharanthus roseus | |
2.1.1.9 | 0.096 | - |
S-adenosyl-L-methionine | when assayed with benzene thiol and furfuryl thiol | Catharanthus roseus | |
2.1.1.9 | 0.218 | - |
benzene thiol | - |
Catharanthus roseus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.9 | Catharanthus roseus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.9 | to near homogeneity by Ni-chelation affinity chromatography | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.9 | 1,3-hexanediol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | 2-mercaptoacetic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | 2-mercaptopropanoic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | 3-mercaptohexan-1-ol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | 3-mercaptopropanoic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | 6-mercaptohexan-1-ol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | benzene thiol + S-adenosyl-L-methionine | - |
Catharanthus roseus | methyl phenyl sulfide + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | dithiothreitol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | ethanethioic S-acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | S-methylethanethioate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | furfuryl thiol + S-adenosyl-L-methionine | - |
Catharanthus roseus | 2-[(methylsulfanyl)methyl]furan + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | guaiacol + S-adenosyl-L-methionine | - |
Catharanthus roseus | 1,2-dimethoxybenzene + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.9 | additional information | no activity with flavonoids and hydroxyl group-containing compounds, inactive with Na2S and 3-mercaptobenzoic acid, presence of carboxyl groups abolishes CrSMT1 activity, threitol, furfuryl alcohol, benzoic acid, 1,3-propandiol, 1,6-hexanediol and pyrocatechol possess negligible activity | Catharanthus roseus | ? | - |
? | |
2.1.1.9 | phenol + S-adenosyl-L-methionine | - |
Catharanthus roseus | methoxybenzene + S-adenosyl-L-homocysteine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.9 | CrSMT1 | - |
Catharanthus roseus |
2.1.1.9 | S-methyltransferase 1 | - |
Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.9 | 20 | - |
with furfuryl thiol as substrate | Catharanthus roseus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.9 | 10 | 30 | with furfuryl thiol as substrate | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.9 | 9 | 9.3 | with furfuryl thiol as substrate | Catharanthus roseus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.9 | 6.5 | 11 | with furfuryl thiol as substrate | Catharanthus roseus |