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Literature summary extracted from

  • Welin, M.; Wang, L.; Eriksson, S.; Eklund, H.
    Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity (2007), J. Mol. Biol., 366, 1615-1623.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.76 in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site Mycoplasma mycoides
2.7.1.76 sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN Mycoplasma mycoides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.76 dATP feedback inhibitor Mycoplasma mycoides
2.7.1.76 dCTP feedback inhibitor Mycoplasma mycoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.76 25000
-
 2 * 25000, Superdex 75 column gel filtration Mycoplasma mycoides
2.7.1.76 50000
-
 Superdex 75 column gel filtration Mycoplasma mycoides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.76 Mycoplasma mycoides
-
 subspecies Mycoplasma mycoides mycoides
-
2.7.1.76 Mycoplasma mycoides Q93IG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.76 Superdex 75 column gel filtration Mycoplasma mycoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.76 ATP + 1-beta-D-arabinofuranosyl cytosine 3% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 1-beta-D-arabinofuranosyl cytosine 3% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 1-beta-D-arabinofuranosyl uracil 0.3% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate
-
 ?
2.7.1.76 ATP + 2',2'-difluoro-2'-deoxycytidine 20% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate
-
 ?
2.7.1.76 ATP + 2',2'-difluoro-2'-deoxycytidine 20% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2',2'-difluoro-2'-deoxyguanosine 24% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate
-
 ?
2.7.1.76 ATP + 2',2'-difluoro-2'-deoxyguanosine 24% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxyadenosine 1.5% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxyadenosine monophosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxyadenosine 1.5% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxyadenosine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxycytidine 0.1% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxycytidine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxyguanosine 0.5% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxyguanosine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxyinoosine 3.9% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxyinosine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 2',3'-dideoxyinosine 3.9% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2',3'-dideoxyinosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxyadenosine
-
 Mycoplasma mycoides ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxycytidine 81% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxyguanosine 97% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxyinosine 116% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-deoxyinosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxythymidine 0.01% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxyuridine 0.9% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-fluoro-arabinosyl adenine 15% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2'-fluoro-arabinosyl adenine monophosphate
-
 ?
2.7.1.76 ATP + 2'-fluoro-arabinosyl adenine 15% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 2-chloro-2'-deoxyadenosine 111% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 2-chloro-2'-deoxyadenosine monophosphate
-
 ?
2.7.1.76 ATP + 2-chloro-2'-deoxyadenosine 111% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 9-beta-D-arabinofuranosyl adenine 77% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 9-beta-D-arabinofuranosyl adenine 77% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate
-
 ?
2.7.1.76 ATP + 9-beta-D-arabinofuranosyl guanine 6% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate
-
 ?
2.7.1.76 ATP + 9-beta-D-arabinofuranosyl guanine 6% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate
-
 ?
2.7.1.76 ATP + adenosine 7.4% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + AMP
-
 ?
2.7.1.76 ATP + adenosine 7.4% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + adenosine 5'-monophosphate
-
 ?
2.7.1.76 ATP + cytidine 0.1% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + cytidine 5'-monophosphate
-
 ?
2.7.1.76 ATP + deoxyadenosine 100% activity Mycoplasma mycoides ADP + dAMP
-
 ?
2.7.1.76 ATP + deoxycytosine 81% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + dCMP
-
 ?
2.7.1.76 ATP + deoxyguanosine 97% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + dGMP
-
 ?
2.7.1.76 ATP + deoxyinosine 116% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + dIMP
-
 ?
2.7.1.76 ATP + deoxyuridine 0.9% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + dUMP
-
 ?
2.7.1.76 ATP + guanosine 5.5% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + GMP
-
 ?
2.7.1.76 ATP + guanosine 5.5% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + guanosine 5'-monophosphate
-
 ?
2.7.1.76 ATP + inosine 5.9% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + IMP
-
 ?
2.7.1.76 ATP + inosine 5.9% of the activity with 2'-deoxyadenosine Mycoplasma mycoides ADP + inosine monophosphate
-
 ?
2.7.1.76 ATP + penciclovir 1.4% relative activity compared to deoxyadenosine Mycoplasma mycoides ADP + ?
-
 ?
2.7.1.76 CTP + deoxyadenosine 57% activity compared to ATP Mycoplasma mycoides CDP + dAMP
-
 ?
2.7.1.76 dATP + deoxyadenosine 2% activity compared to ATP Mycoplasma mycoides dADP + dAMP
-
 ?
2.7.1.76 dCTP + deoxyadenosine 106% activity compared to ATP Mycoplasma mycoides dCDP + dAMP
-
 ?
2.7.1.76 dTTP + deoxyadenosine 44% activity compared to ATP Mycoplasma mycoides dTDP + dAMP
-
 ?
2.7.1.76 additional information less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine Mycoplasma mycoides ?
-
 ?
2.7.1.76 UTP + deoxyadenosine 22% activity compared to ATP Mycoplasma mycoides UDP + dAMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.76 dimer 2 * 25000, Superdex 75 column gel filtration Mycoplasma mycoides

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 dAK
-
 Mycoplasma mycoides

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.76 ATP
-
 Mycoplasma mycoides