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Literature summary extracted from

  • Vasdev, K.; Dhawan, S.; Kapoor, R.K.; Kuhad, R.C.
    Biochemical characterization and molecular evidence of a laccase from the birds nest fungus Cyathus bulleri (2005), Fungal Genet. Biol., 42, 684-693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 DNA and amino acid sequence determination and analysis Cyathus bulleri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol competitive, complete inhibition Cyathus bulleri
1.10.3.2 cetyltrimethyl ammonium bromide 50% inhibition Cyathus bulleri
1.10.3.2 HgCl2 competitive, complete inhibition Cyathus bulleri
1.10.3.2 KCN competitive, complete inhibition Cyathus bulleri
1.10.3.2 NaN3 competitive, complete inhibition Cyathus bulleri
1.10.3.2 SDS competitive, complete inhibition Cyathus bulleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 14
-
 ferulic acid pH 5.2, 45°C Cyathus bulleri
1.10.3.2 22
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
1.10.3.2 48.6
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.2, 45°C Cyathus bulleri
1.10.3.2 56
-
 2,6-dimethoxyphenol pH 5.2, 45°C Cyathus bulleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ the gene sequence encodes copper-binding domains I and II Cyathus bulleri
1.10.3.2 additional information no effect by EDTA Cyathus bulleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 60000
-
 gel filtration Cyathus bulleri

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cyathus bulleri Q8X1D1 birds nest fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 95.7fold to homogeneity by anion exchange chromatography and gel filtration Cyathus bulleri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Cyathus bulleri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 226
-
 purified enzyme Cyathus bulleri

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 4°C, purified enzyme, 50 mM citrate phosphate, pH 5.0, no loss in activity after 1 month Cyathus bulleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Cyathus bulleri 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 3,4-dihydroxybenzoic acid + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 additional information substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid Cyathus bulleri ?
-
 ?
1.10.3.2 syringaldehyde + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 vanillin + O2
-
 Cyathus bulleri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 60000, SDS-PAGE Cyathus bulleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
-
 Cyathus bulleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 25 70 stable at, inactivation above Cyathus bulleri
1.10.3.2 80
-
 complete inactivation Cyathus bulleri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 5.2
-
 ferulic acid pH 5.2, 45°C Cyathus bulleri
1.10.3.2 5.5
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
1.10.3.2 95.6
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
1.10.3.2 180
-
 2,6-dimethoxyphenol pH 5.2, 45°C Cyathus bulleri
1.10.3.2 204
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.2, 45°C Cyathus bulleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5.2
-
-
 Cyathus bulleri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 6 sharp decline in activity above pH 6.0 Cyathus bulleri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Cyathus bulleri isoelectric focusing
-
 3.7