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Literature summary extracted from

  • Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K.
    Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain 10d: a different modified meta-cleavage pathway for 2-aminophenols (2006), Biosci. Biotechnol. Biochem., 70, 2653-2661.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.32 CuSO4 0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM Bordetella sp.
1.2.1.32 HgCl2 0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM Bordetella sp.
1.2.1.32 iodoacetic acid 31% inhibition at 1 mM; 69% remaining activity at an inhibitor concentration of 1 mM Bordetella sp.
1.2.1.32 N-ethylmaleimide 18% remaining activity at an inhibitor concentration of 1 mM Bordetella sp.
1.2.1.32 N-Methylmaleimide 82% inhibition at 1 mM Bordetella sp.
1.2.1.32 p-chloromercuribenzoic acid 41% remaining activity at an inhibitor concentration of 1 mM; 59% inhibition at 1 mM Bordetella sp.
1.2.1.32 ZnSO4 26% remaining activity at an inhibitor concentration of 1 mM; 74% inhibition at 1 mM Bordetella sp.
1.2.1.85 Cu2+ complete inhibition at 1 mM Bordetella sp.
1.2.1.85 Hg2+ complete inhibition at 1 mM Bordetella sp.
1.2.1.85 iodoacetic acid 69% residual activity at 1 mM Bordetella sp.
1.2.1.85 N-ethylmaleimide 18% residual activity at 1 mM Bordetella sp.
1.2.1.85 p-chloromercuribenzoate 41% residual activity at 1 mM Bordetella sp.
1.2.1.85 Zn2+ 26% residual activity at 1 mM Bordetella sp.
4.1.1.77 CoCl2 1 mM, 47% remaining activity Bordetella sp.
4.1.1.77 FeSO4 1 mM, 23% remaining activity Bordetella sp.
4.1.1.77 iodoacetic acid 1 mM, 64% remaining activity Bordetella sp.
4.1.1.77 K3Fe(CN)6 1 mM, 36% remaining activity Bordetella sp.
4.1.1.77 MnSO4 1 mM, 9% remaining activity Bordetella sp.
4.1.1.77 N-Methylmaleimide 1 mM, 58% remaining activity Bordetella sp.
4.1.1.77 p-chloromercuribenzoic acid 1 mM, 54% remaining activity Bordetella sp.
4.1.1.77 Tiron 1 mM, 59% remaining activity Bordetella sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.77 Mg2+ required Bordetella sp.
4.2.1.80 Mg2+ required Bordetella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.77 29000
-
 SDS-PAGE Bordetella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O Bordetella sp.
-
 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O Bordetella sp.
-
 2-hydroxymuconate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Bordetella sp.
-
-
-
1.2.1.32 Bordetella sp.
-
 Bordetella sp. strain 10d
-
1.2.1.32 Bordetella sp. 10d
-
-
-
1.2.1.85 Bordetella sp.
-
-
-
1.2.1.85 Bordetella sp. 10d
-
-
-
4.1.1.77 Bordetella sp.
-
-
-
4.1.1.77 Bordetella sp. 10d
-
-
-
4.2.1.80 Bordetella sp.
-
-
-
4.2.1.80 Bordetella sp. 10d
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.32 ammonium sulfate fractionation, chromatography on DE52 cellulose, chromatography on DEAE-cellulofine, chromatography on DEAE-toyopearl Bordetella sp.
1.2.1.32 ammonium sulfate precipitation followed by 3 chromatography steps Bordetella sp.
1.2.1.85 streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography Bordetella sp.
4.1.1.77 DE52 cellulose column chromatography and DEAE-Cellulofine A800 column chromatography, DEAE-Toyopearl column chromatography, streptomycin sulfate precipitation, and ammonium sulfate precipitation Bordetella sp.
4.2.1.80
-
 Bordetella sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.32 cell culture
-
 Bordetella sp.
-
1.2.1.85 culture condition:4-amino-3-hydroxybenzoate-grown cell
-
 Bordetella sp.
-
1.2.1.85 culture condition:succinate-glucose-grown cell
-
 Bordetella sp.
-
4.1.1.77 culture condition:4-amino-3-hydroxybenzoate-grown cell
-
 Bordetella sp.
-
4.2.1.80 additional information high activity when the organism is cultivated in medium containing 0.12% (w/v) 4-amino-3-hydroxybenzoic acid as sole carbon, nitrogen, and energy source until the late exponential phase. Low activity when the organism is cultivated in succinate-glucose medium until the late-exponential phase Bordetella sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.32 0.0037
-
 after streptomycin sulfate treatment Bordetella sp.
1.2.1.32 0.0041
-
 cell extract Bordetella sp.
1.2.1.32 0.0092
-
 after ammonium sulfate precipitation Bordetella sp.
1.2.1.32 0.046
-
 after DE52 chromatography Bordetella sp.
1.2.1.32 0.071
-
 after DEAE-Toyopearl chromatography Bordetella sp.
1.2.1.32 0.075
-
 after DEAE-Cellulofine chromatography Bordetella sp.
1.2.1.85 additional information
-
 enzyme from cell extract shows a specific activity of 0.0041 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C. After 17fold purification, the enzyme shows a specific activity of 0.071 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C, in 100 mM sodium phosphate, pH 7.5, at 24°C Bordetella sp.
4.1.1.77 0.05
-
 cell extract Bordetella sp.
4.1.1.77 18
-
 after 360fold purification Bordetella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Bordetella sp. 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Bordetella sp. 2-hydroxymuconate + NADH
-
 ?
1.2.1.32 2-hydroxymuconic 6-semialdehyde + NAD+ + H2O substrate for spectrophotometric activity assay, relative activity 100% Bordetella sp. 2-hydroxymuconate + NADH + H+
-
 ?
1.2.1.32 acetaldehyde + NAD+ + H2O
-
 Bordetella sp. acetate + NADH + H+
-
 ?
1.2.1.32 acetaldehyde + NAD+ + H2O relative activity 113% Bordetella sp. acetate + NADH + H+
-
 ?
1.2.1.32 acetaldehyde + NAD+ + H2O
-
 Bordetella sp. 10d acetate + NADH + H+
-
 ?
1.2.1.32 acetaldehyde + NAD+ + H2O relative activity 113% Bordetella sp. 10d acetate + NADH + H+
-
 ?
1.2.1.32 benzaldehyde + NAD+ + H2O
-
 Bordetella sp. benzoate + NADH + H+
-
 ?
1.2.1.32 benzaldehyde + NAD+ + H2O relative activity 25% Bordetella sp. benzoate + NADH + H+
-
 ?
1.2.1.32 butyraldehyde + NAD+ + H2O relative activity 138% Bordetella sp. butyrate + NADH + H+
-
 ?
1.2.1.32 butyraldehyde + NAD+ + H2O relative activity 138% Bordetella sp. 10d butyrate + NADH + H+
-
 ?
1.2.1.32 formaldehyde + NAD+ + H2O
-
 Bordetella sp. formate + NADH
-
 ?
1.2.1.32 formaldehyde + NAD+ + H2O relative activity 16,5% Bordetella sp. formate + NADH + H+
-
 ?
1.2.1.32 n-butanal + NAD+ + H2O
-
 Bordetella sp. n-butanoate + NADH + H+
-
 ?
1.2.1.32 propanal + NAD+ + H2O
-
 Bordetella sp. n-propanoate + NADH + H+
-
 ?
1.2.1.32 propionaldehyde + NAD+ + H2O relative activity 107% Bordetella sp. propionate + NADH + H+
-
 ?
1.2.1.32 propionaldehyde + NAD+ + H2O relative activity 107% Bordetella sp. 10d propionate + NADH + H+
-
 ?
1.2.1.85 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O 100% activity Bordetella sp. (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
 ?
1.2.1.85 acetaldehyde + NAD+ + H2O 113% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. acetate + NADH + H+
-
 ?
1.2.1.85 benzaldehyde + NAD+ + H2O 25% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. benzoate + NADH + H+
-
 ?
1.2.1.85 butyraldehyde + NAD+ + H2O 138% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. butanoate + NADH + H+
-
 ?
1.2.1.85 formaldehyde + NAD+ + H2O 16.5% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. formate + NADH + H+
-
 ?
1.2.1.85 propionaldehyde + NAD+ + H2O 107% activity compared to 2-hydroxymuconate-6-semialdehyde Bordetella sp. propanoate + NADH + H+
-
 ?
4.1.1.77 4-Oxalocrotonate
-
 Bordetella sp. 2-Oxopent-4-enoate + CO2
-
 ?
4.1.1.77 4-Oxalocrotonate
-
 Bordetella sp. 10d 2-Oxopent-4-enoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.80 More 4-oxocorotonate decarboxylase and 2-oxopent-4-enoate hydratase from strain 10 days form a complex in a molar ratio 1:1 Bordetella sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.32 2-hydroxymuconic 6-semialdehyde dehydrogenase
-
 Bordetella sp.
1.2.1.32 aminomuconate-semialdehyde dehydrogenase
-
 Bordetella sp.
1.2.1.85 NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
-
 Bordetella sp.
4.1.1.77 4OD
-
 Bordetella sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.32 NAD+
-
 Bordetella sp.