Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vinogradov, V.; Vyazmensky, M.; Engel, S.; Belenky, I.; Kaplun, A.; Kryukov, O.; Barak, Z.; Chipman, D.M.
    Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties (2006), Biochim. Biophys. Acta, 1760, 356-363.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 isozyme I subunit-encoding genes ilvB and ilvN, DNA and amino acid sequence determination and analysis, expression of His-tagged holoenzyme or individual subunits in strain BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 benzaldehyde inhibits isozyme AHAS II, not isozyme AHAS I Escherichia coli
2.2.1.6 additional information inhibition kinetics or recombinant wild.type and reconstituted isozymes AHAS I Escherichia coli
2.2.1.6 valine isozyme AHAS I, cooperative feedback inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 additional information
-
 additional information kinetics or recombinant wild-type and reconstituted isozymes AHAS I, exclusive binding model Escherichia coli
2.2.1.6 4
-
 pyruvate 37°C, reconstituted, recombinant holoenzyme Escherichia coli
2.2.1.6 4.8
-
 pyruvate 37°C, recombinant holoenzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 additional information Escherichia coli mechanism of expression regulation, overview ?
-
 ?
2.2.1.6 pyruvate Escherichia coli first step in the biosynthesis of valine, overview 2-acetolactate + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Escherichia coli
-
 isozymes I-III, subunit-encoding genes ilvB and ilvN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 recombinant His-tagged isozyme I holoenzyme or individual subunits from strain BL21 by affinity chromatography Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.2.1.6 2 pyruvate = 2-acetolactate + CO2 the enzyme catalyzes the decarboxylation of bound pyruvate to form a hydroxyethyl-thiamine diphosphate anion/enamine intermediate, which normally reacts with a second molecule of an alpha-ketoacid to form an acetohydroxyacid, but can also perform several side reactions proceeding through the hydroxyethyl-thiamine diphosphate anion/enamine intermediate Escherichia coli
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.2.1.6 reconstitution of the holoenzyme from recombinantly expressed, purified subunits encoded by genes ilvB and ilvN Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 additional information
-
-
 Escherichia coli
2.2.1.6 60.5
-
 reconstituted, recombinant holoenzyme Escherichia coli
2.2.1.6 63.6
-
 recombinant holoenzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 additional information mechanism of expression regulation, overview Escherichia coli ?
-
 ?
2.2.1.6 additional information acetohydroxybutyrate is preferably formed, isozyme AHAS I can also form peracetate from synthetic acetolactate Escherichia coli ?
-
 ?
2.2.1.6 pyruvate
-
 Escherichia coli 2-acetolactate + CO2
-
 r
2.2.1.6 pyruvate first step in the biosynthesis of valine, overview Escherichia coli 2-acetolactate + CO2
-
 r
2.2.1.6 pyruvate + benzaldehyde stereospecific reaction, isozymes AHAS I and II Escherichia coli (R)-phenylacetylcarbinol + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Escherichia coli
2.2.1.6 AHAS
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD
-
 Escherichia coli
2.2.1.6 thiamine diphosphate dependent on Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 1.4
-
 benzaldehyde isozyme AHAS II Escherichia coli