EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.36 | into the cloning vector pGEM-T Easy and subcloned into the expression vector pET14b | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.36 | F471L | mutant | Mus musculus |
1.2.1.36 | T1411C | mutant | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.36 | 3,3',5-tri-iodothyronine | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.36 | 0.0002 | - |
all-trans-retinal | wild-type | Mus musculus | |
1.2.1.36 | 0.0002 | - |
all-trans-retinal | F471L mutant | Mus musculus | |
1.2.1.36 | 0.0007 | - |
octanal | wild-type | Mus musculus | |
1.2.1.36 | 0.0009 | - |
octanal | F471L mutant | Mus musculus | |
1.2.1.36 | 0.0065 | - |
decanal | wild-type | Mus musculus | |
1.2.1.36 | 0.0221 | - |
hexanal | wild-type | Mus musculus | |
1.2.1.36 | 0.0431 | - |
NAD+ | F471L mutant | Mus musculus | |
1.2.1.36 | 0.0528 | - |
NAD+ | wild-type | Mus musculus | |
1.2.1.36 | 0.103 | - |
benzaldehyde | wild-type | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.36 | retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.36 | Mus musculus | Q9JHW9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.36 | using a nickel-chelating Sepharose and a S300 HR gel-filtration column | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.36 | benzaldehyde + NAD+ + H2O | - |
Mus musculus | benzoate + NADH + H+ | - |
? | |
1.2.1.36 | decanal + NAD+ + H2O | - |
Mus musculus | decanoate + NADH + H+ | - |
? | |
1.2.1.36 | hexanal + NAD+ + H2O | - |
Mus musculus | hexanoate + NADH + H+ | - |
? | |
1.2.1.36 | octanal + NAD+ + H2O | - |
Mus musculus | octanoate + NADH + H+ | - |
? | |
1.2.1.36 | retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.36 | RALDH3 | - |
Mus musculus |
1.2.1.36 | Retinal dehydrogenase | - |
Mus musculus |
1.2.1.36 | retinaldehyde dehydrogenase 3 | - |
Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.36 | 37 | - |
activity assay | Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.36 | 0.2 | - |
benzaldehyde | wild-type | Mus musculus | |
1.2.1.36 | 0.3 | - |
hexanal | wild-type | Mus musculus | |
1.2.1.36 | 0.4 | - |
decanal | wild-type | Mus musculus | |
1.2.1.36 | 3.1 | - |
NAD+ | F471L mutant | Mus musculus | |
1.2.1.36 | 3.3 | - |
NAD+ | wild-type | Mus musculus | |
1.2.1.36 | 3.9 | - |
retinal | all-trans-retinal, wild-type | Mus musculus | |
1.2.1.36 | 4.8 | - |
octanal | wild-type | Mus musculus | |
1.2.1.36 | 5.4 | - |
octanal | F471L mutant | Mus musculus | |
1.2.1.36 | 13 | - |
retinal | all-trans-retinal, F471L mutant | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.36 | 8.5 | - |
activity assay | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.36 | NADH | - |
Mus musculus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.36 | 0.0377 | - |
3,3',5-tri-iodothyronine | - |
Mus musculus |