EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.7 | expression in Emericella nidulans under control of the fungal alc promoter | Pleurotus eryngii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.7 | 3-Phenyl-1-propanol | competitive | Pleurotus eryngii | |
1.1.3.7 | 4-anisic acid | competitive | Pleurotus eryngii | |
1.1.3.7 | 4-methoxybenzylamine | uncompetitive, pH-dependent inhibition, best at pH 8.0 | Pleurotus eryngii | |
1.1.3.7 | benzylmethyl ether | competitive | Pleurotus eryngii | |
1.1.3.7 | chavicol | competitive | Pleurotus eryngii | |
1.1.3.7 | phenol | competitive | Pleurotus eryngii | |
1.1.3.7 | Toluene | competitive | Pleurotus eryngii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.7 | additional information | - |
additional information | stopped-flow and steady-state kinetics | Pleurotus eryngii | |
1.1.3.7 | 0.014 | - |
3-chloro-4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.027 | - |
4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.094 | - |
2,4-hexadien-1-ol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.107 | - |
3-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.132 | - |
4-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.227 | - |
3-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.54 | - |
veratryl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.553 | - |
4-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.554 | - |
3-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.632 | - |
benzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.708 | - |
cinnamyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.831 | - |
isovanillyl alcohol | pH 6.0, 24°C | Pleurotus eryngii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.7 | benzyl alcohol + O2 | Pleurotus eryngii | - |
benzaldehyde + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.7 | Pleurotus eryngii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.7 | recombinant enzyme from Emericella nidulans by gel filtration and anion exchange chromatography | Pleurotus eryngii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.7 | an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 | the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, the enzyme does not thermodynamically stabilize a flavin semiquinone radical and forms no sulphite adduct | Pleurotus eryngii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | additional information | - |
- |
Pleurotus eryngii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.7 | 2,4-hexadien-1-ol + O2 | high activity | Pleurotus eryngii | 2,4-hexadienal + H2O2 | - |
? | |
1.1.3.7 | 3-anisyl alcohol + O2 | - |
Pleurotus eryngii | 3-anisyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 3-chloro-4-anisyl alcohol + O2 | high activity | Pleurotus eryngii | 3-chloro-4-anisyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 3-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 3-chlorobenzyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 3-fluorobenzyl alcohol + O2 | low activity | Pleurotus eryngii | 3-fluorobenzyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 4-anisyl alcohol + O2 | best substrate | Pleurotus eryngii | 4-anisyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 4-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-chlorobenzyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | 4-fluorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-fluorobenzyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | benzyl alcohol + O2 | - |
Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
1.1.3.7 | benzyl alcohol + O2 | the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, overview | Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
1.1.3.7 | cinnamyl alcohol + O2 | - |
Pleurotus eryngii | cinnamyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | isovanillyl alcohol + O2 | - |
Pleurotus eryngii | isovanillyl aldehyde + H2O2 | - |
? | |
1.1.3.7 | additional information | 4-(hydroxymethyl)-benzoic acid is a poor substrate | Pleurotus eryngii | ? | - |
? | |
1.1.3.7 | veratryl alcohol + O2 | - |
Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | 24 | - |
assay at | Pleurotus eryngii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | 47 | - |
90% of maximal activity within this range | Pleurotus eryngii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.7 | 6 | - |
3-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 15 | - |
3-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 22 | - |
3-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 30 | - |
benzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 32 | - |
4-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 46 | - |
3-chloro-4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 51 | - |
4-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 65 | - |
cinnamyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 114 | - |
veratryl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 119 | - |
2,4-hexadien-1-ol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 127 | - |
isovanillyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 142 | - |
4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | 6 | - |
- |
Pleurotus eryngii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | 2 | 11 | - |
Pleurotus eryngii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.7 | 6 | 9 | very stable | Pleurotus eryngii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.7 | FAD | flavoenzyme, involved in the catalytic reaction | Pleurotus eryngii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.7 | additional information | - |
additional information | inhibition kinetcis | Pleurotus eryngii | |
1.1.3.7 | 0.08 | - |
4-anisic acid | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.11 | - |
chavicol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.25 | - |
4-methoxybenzylamine | pH 8.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 0.75 | - |
Toluene | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 1.35 | - |
benzylmethyl ether | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 1.92 | - |
phenol | pH 6.0, 24°C | Pleurotus eryngii | |
1.1.3.7 | 4.48 | - |
3-Phenyl-1-propanol | pH 6.0, 24°C | Pleurotus eryngii |