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Literature summary extracted from

  • Peng, X.; Shindo, K.; Kanoh, K.; Inomata, Y.; Choi, S.K.; Misawa, N.
    Characterization of Sphingomonas aldehyde dehydrogenase catalyzing the conversion of various aromatic aldehydes to their carboxylic acids (2005), Appl. Microbiol. Biotechnol., 69, 141-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expression in Escherichia coli JM109 Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Sphingomonas sp. Q402C7 strain 14DN61
-
1.2.1.3 Sphingomonas sp. 14DN-61 Q402C7 strain 14DN61
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 Ni-affinity chromatography Sphingomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 cell culture
-
 Sphingomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 0.0636
-
 substrate: o-methoxybenzaldehyde Sphingomonas sp.
1.2.1.3 0.0825
-
 substrate: o-chlorobenzaldehyde Sphingomonas sp.
1.2.1.3 0.09
-
 substrate: o-tolualdehyde Sphingomonas sp.
1.2.1.3 0.1029
-
 substrate: p-hydroxybenzaldehyde Sphingomonas sp.
1.2.1.3 0.11
-
 substrate: protocatechaldehyde Sphingomonas sp.
1.2.1.3 0.1516
-
 substrate: p-methoxybenzaldehyde Sphingomonas sp.
1.2.1.3 0.3812
-
 substrate: cinnamaldehyde Sphingomonas sp.
1.2.1.3 0.4366
-
 substrate: o-nitrobenzaldehyde Sphingomonas sp.
1.2.1.3 0.5811
-
 substrate: m-hydroxybenzaldehyde Sphingomonas sp.
1.2.1.3 1.1
-
 substrate: m-chlorobenzaldehyde Sphingomonas sp.
1.2.1.3 1.15
-
 substrate: m-nitrobenzaldehyde Sphingomonas sp.
1.2.1.3 1.154
-
 substrate: 1-naphthaldehyde Sphingomonas sp.
1.2.1.3 1.2
-
 substrate: 2-naphthaldehyde Sphingomonas sp.
1.2.1.3 1.292
-
 substrate: m-fluorobenzaldehyde Sphingomonas sp.
1.2.1.3 1.53
-
 substrate: vanillin Sphingomonas sp.
1.2.1.3 1.599
-
 substrate: syringaldehyde Sphingomonas sp.
1.2.1.3 1.601
-
 substrate: o-hydroxybenzaldehyde Sphingomonas sp.
1.2.1.3 1.61
-
 substrate: p-fluorobenzaldehyde Sphingomonas sp.
1.2.1.3 1.696
-
 substrate: p-nitrobenzaldehyde Sphingomonas sp.
1.2.1.3 1.739
-
 substrate: m-tolualdehyde Sphingomonas sp.
1.2.1.3 1.815
-
 substrate: beznaldehyde Sphingomonas sp.
1.2.1.3 1.957
-
 substrate: p-chlorobenzaldehyde Sphingomonas sp.
1.2.1.3 2.006
-
 substrate: o-fluorobenzaldehyde Sphingomonas sp.
1.2.1.3 2.175
-
 substrate: m-methoxybenzaldehyde Sphingomonas sp.
1.2.1.3 2.457
-
 substrate: p-tolualdehyde Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 1-naphthaldehyde + NAD+ + H2O 46.9% activity compared to p-tolualdehyde Sphingomonas sp. 1-naphthoic acid + NADH
-
 ?
1.2.1.3 2-naphthaldehyde + NAD+ + H2O 48.8% activity compared to p-tolualdehyde Sphingomonas sp. 2-naphthoic acid + NADH
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 73.9% activity compared to p-tolualdehyde Sphingomonas sp. benzoate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 73.9% activity compared to p-tolualdehyde Sphingomonas sp. 14DN-61 benzoate + NADH + H+
-
 ?
1.2.1.3 cinnamaldehyde + NAD+ + H2O 15.5% activity compared to p-tolualdehyde Sphingomonas sp. cinnamate + NADH + H+
-
 ?
1.2.1.3 cinnamaldehyde + NAD+ + H2O 15.5% activity compared to p-tolualdehyde Sphingomonas sp. 14DN-61 cinnamate + NADH + H+
-
 ?
1.2.1.3 m-chlorobenzaldehyde + NAD+ + H2O 44.7% activity compared to p-tolualdehyde Sphingomonas sp. m-chlorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 m-fluorobenzaldehyde + NAD+ + H2O 52.6% activity compared to p-tolualdehyde Sphingomonas sp. m-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 m-fluorobenzaldehyde + NAD+ + H2O 52.6% activity compared to p-tolualdehyde Sphingomonas sp. 14DN-61 m-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 m-hydroxybenzaldehyde + NAD+ + H2O 23.6% activity compared to p-tolualdehyde Sphingomonas sp. m-hydroxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 m-methoxybenzaldehyde + NAD+ + H2O 88.5% activity compared to p-tolualdehyde Sphingomonas sp. m-methoxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 m-nitrobenzaldehyde + NAD+ + H2O 46.8% activity compared to p-tolualdehyde Sphingomonas sp. m-nitrobenzoic acid + NADH
-
 ?
1.2.1.3 m-tolualdehyde + NAD+ + H2O 70.8% activity compared to p-tolualdehyde Sphingomonas sp. m-methylbenzoic acid + NADH
-
 ?
1.2.1.3 o-chlorobenzaldehyde + NAD+ + H2O 3.4% activity compared to p-tolualdehyde Sphingomonas sp. o-chlorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 o-fluorobenzaldehyde + NAD+ + H2O 81.6% activity compared to p-tolualdehyde Sphingomonas sp. o-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 o-fluorobenzaldehyde + NAD+ + H2O 81.6% activity compared to p-tolualdehyde Sphingomonas sp. 14DN-61 o-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 o-hydroxybenzaldehyde + NAD+ + H2O 65.2% activity compared to p-tolualdehyde Sphingomonas sp. o-hydroxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 o-methoxybenzaldehyde + NAD+ + H2O 2.6% activity compared to p-tolualdehyde Sphingomonas sp. o-methoxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 o-nitrobenzaldehyde + NAD+ + H2O 17.8% activity compared to p-tolualdehyde Sphingomonas sp. o-nitrobenzoic acid + NADH
-
 ?
1.2.1.3 o-tolualdehyde + NAD+ + H2O 3.7% activity compared to p-tolualdehyde Sphingomonas sp. o-methylbenzoic acid + NADH
-
 ?
1.2.1.3 p-chlorobenzaldehyde + NAD+ + H2O 79.6% activity compared to p-tolualdehyde Sphingomonas sp. p-chlorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 p-fluorobenzaldehyde + NAD+ + H2O 65.5%activity compared to p-tolualdehyde Sphingomonas sp. p-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 p-fluorobenzaldehyde + NAD+ + H2O 65.5%activity compared to p-tolualdehyde Sphingomonas sp. 14DN-61 p-fluorobenzoic acid + NADH + H+
-
 ?
1.2.1.3 p-hydroxybenzaldehyde + NAD+ + H2O 4.2% activity compared to p-tolualdehyde Sphingomonas sp. p-hydroxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 p-methoxybenzaldehyde + NAD+ + H2O 6.2% activity compared to p-tolualdehyde Sphingomonas sp. p-methoxybenzoic acid + NADH + H+
-
 ?
1.2.1.3 p-nitrobenzaldehyde + NAD+ + H2O 69% activity compared to p-tolualdehyde Sphingomonas sp. p-nitrobenzoic acid + NADH
-
 ?
1.2.1.3 p-tolualdehyde + NAD+ + H2O highest activity Sphingomonas sp. p-methylbenzoic acid + NADH
-
 ?
1.2.1.3 protocatechaldehyde + NAD+ + H2O 4.5% activity compared to p-tolualdehyde Sphingomonas sp. protocatechuic acid + NADH
-
 ?
1.2.1.3 syringaldehyde + NAD+ + H2O 65.1% activity compared to p-tolualdehyde Sphingomonas sp. syringic acid + NADH
-
 ?
1.2.1.3 vanillin + NAD+ + H2O 62.3% activity compared to p-tolualdehyde Sphingomonas sp. vanillic acid + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 aldehyde dehydrogenase
-
 Sphingomonas sp.
1.2.1.3 PhnN
-
 Sphingomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Sphingomonas sp.