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Literature summary extracted from

  • Cafaro, V.; Notomista, E.; Capasso, P.; Di Donato, A.
    Mutation of glutamic acid 103 of toluene o-xylene monooxygenase as a means to control the catabolic efficiency of a recombinant upper pathway for degradation of methylated aromatic compounds (2005), Appl. Environ. Microbiol., 71, 4744-4750.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 co-expression with wild-type or E103 mutant toluene o-xylene monooxygenases in Escherichia coli strain JM109 Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.7 additional information production ToMO mutants with modified regioselectivity compared with the regioselectivity of the wild-type protein in order to alter the ability of the recombinant upper pathway to produce methylcatechol isomers from toluene and to produce 3,4-dimethylcatechol from o-xylene, the combination of ToMO mutant E103G and phenol oxidase increases the production of 4-methylcatechol from toluene and the formation of 3,4-dimethylcatechol from o-xylene, overview Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2 Pseudomonas stutzeri
-
 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2 Pseudomonas stutzeri OX1
-
 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas stutzeri
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas stutzeri OX1
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 phenol + NADPH + O2 Pseudomonas stutzeri
-
 catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2 Pseudomonas stutzeri OX1
-
 catechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Pseudomonas stutzeri
-
-
-
1.14.13.7 Pseudomonas stutzeri OX1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri OX1 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas stutzeri 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas stutzeri OX1 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas stutzeri catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas stutzeri OX1 catechol + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 phenol hydroxylase
-
 Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADPH
-
 Pseudomonas stutzeri