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Literature summary extracted from

  • Olaniran, A.O.; Pillay, D.; Pillay, B.
    Haloalkane and haloacid dehalogenase from aerobic bacterial isolates indigenous to contaminated sites in Africa demonstrate diverse substrate specificities (2004), Chemosphere, 55, 27-33.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.3 HgCl2
-
 Burkholderia sp.
3.8.1.3 iodoacetamide
-
 Burkholderia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.3 Burkholderia sp.
-
-
-
3.8.1.5 Bacillus sp. (in: Bacteria)
-
 isolated from contaminated sites in Africa, growth on 1,2-dichloroethane as sole carbon source
-
3.8.1.5 Burkholderia sp.
-
 isolated from contaminated sites in Africa, growth on 1,2-dichloroethane as sole carbon source
-
3.8.1.5 Corynebacterium sp.
-
 isolated from contaminated sites in Africa, growth on 1,2-dichloroethane as sole carbon source
-
3.8.1.5 Micrococcus sp.
-
 isolated from contaminated sites in Africa, growth on 1,2-dichloroethane as sole carbon source
-
3.8.1.5 Pseudomonas sp.
-
 isolated from contaminated sites in Africa, growth on 1,2-dichloroethane as sole carbon source
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.5 40
-
 substrate trichloroacetic acid, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 59
-
 substrate trichloroacetic acid, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 66
-
 substrate trichloroethane, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 70
-
 substrate trichloroacetic acid, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 70
-
 substrate trichloroethane, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 70
-
 substrate trichloroethane, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 71
-
 substrate monochloroacetic acid, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 71
-
 substrate trichloroacetic acid, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 72
-
 substrate dichloromethane, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 72
-
 substrate tetrachloromethane, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 81
-
 substrate monochloroacetic acid, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 81
-
 substrate tetrachloromethane, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 81
-
 substrate trichloroethane, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 82
-
 substrate monochloroacetic acid, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 82
-
 substrate tetrachloromethane, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 88
-
 substrate trichloroacetic acid, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 94
-
 substrate monochloroacetic acid, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 94
-
 substrate trichloroethane, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 96
-
 substrate trichloromethane, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 105
-
 substrate tetrachloromethane, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 106
-
 substrate dichloromethane, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 106
-
 substrate monochloroacetic acid, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 106
-
 substrate trichloroethane, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 106
-
 substrate trichloromethane, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 108
-
 substrate dichloromethane, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 117
-
 substrate tetrachloromethane, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 117
-
 substrate trichloroethane, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 117
-
 substrate trichloromethane, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 129
-
 substrate dichloromethane, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 129
-
 substrate trichloroethane, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 130
-
 substrate trichloroethane, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 130
-
 substrate trichloromethane, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 130
-
 substrate trichloromethane, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 141
-
 substrate dichloromethane, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 141
-
 substrate tetrachloromethane, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 141
-
 substrate trichloroethane, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 151
-
 substrate tetrachloromethane, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 153
-
 substrate 1,2-dichloroethane, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 153
-
 substrate dichloromethane, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 153
-
 substrate dichloromethane, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 153
-
 substrate tetrachloromethane, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 153
-
 substrate trichloromethane, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 164
-
 substrate 1,2-dichloroethane, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 164
-
 substrate dichloromethane, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 164
-
 substrate trichloromethane, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 166
-
 substrate 1,2-dichloroethane, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 175
-
 substrate dichloromethane, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 176
-
 substrate 1,2-dichloroethane, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 176
-
 substrate tetrachloromethane, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 176
-
 substrate tetrachloromethane, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 176
-
 substrate trichloromethane, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 188
-
 substrate trichloromethane, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 188
-
 substrate trichloromethane, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 200
-
 substrate dichloromethane, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 200
-
 substrate trichloroacetic acid, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 211
-
 substrate 1,2-dichloroethane, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 223
-
 substrate 1,2-dichloroethane, 35°C, pH 7.5, activity of crude extract Corynebacterium sp.
3.8.1.5 223
-
 substrate trichloroacetic acid, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 235
-
 substrate trichloroacetic acid, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 247
-
 substrate trichloroacetic acid, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 258
-
 substrate 1,2-dichloroethane, 35°C, pH 7.5, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 258
-
 substrate trichloroacetic acid, 35°C, pH 9.0, activity of crude extract Burkholderia sp.
3.8.1.5 270
-
 substrate 1,2-dichloroethane, 35°C, pH 7.5, activity of crude extract Micrococcus sp.
3.8.1.5 282
-
 substrate 1,2-dichloroethane, 35°C, pH 7.5, activity of crude extract Pseudomonas sp.
3.8.1.5 282
-
 substrate monochloroacetic acid, 35°C, pH 9.0, activity of crude extract Corynebacterium sp.
3.8.1.5 294
-
 substrate 1,2-dichloroethane, 35°C, pH 7.5, activity of crude extract Burkholderia sp.
3.8.1.5 317
-
 substrate monochloroacetic acid, 35°C, pH 9.0, activity of crude extract Bacillus sp. (in: Bacteria)
3.8.1.5 329
-
 substrate monochloroacetic acid, 35°C, pH 9.0, activity of crude extract Micrococcus sp.
3.8.1.5 346
-
 substrate monochloroacetic acid, 35°C, pH 9.0, activity of crude extract Pseudomonas sp.
3.8.1.5 352
-
 substrate monochloroacetic acid, 35°C, pH 9.0, activity of crude extract Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.3 monochloroacetate + H2O
-
 Burkholderia sp. glycolate + HCl
-
 ?
3.8.1.5 1,1,2-trichloroethane + H2O
-
 Pseudomonas sp. ? + chloride
-
 ?
3.8.1.5 1,1,2-trichloroethane + H2O
-
 Corynebacterium sp. ? + chloride
-
 ?
3.8.1.5 1,1,2-trichloroethane + H2O
-
 Bacillus sp. (in: Bacteria) ? + chloride
-
 ?
3.8.1.5 1,1,2-trichloroethane + H2O
-
 Micrococcus sp. ? + chloride
-
 ?
3.8.1.5 1,1,2-trichloroethane + H2O
-
 Burkholderia sp. ? + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Pseudomonas sp. 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Corynebacterium sp. 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Bacillus sp. (in: Bacteria) 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Micrococcus sp. 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Burkholderia sp. 2-chloroethanol + chloride
-
 ?
3.8.1.5 dichloromethane + H2O
-
 Pseudomonas sp. ? + chloride
-
 ?
3.8.1.5 dichloromethane + H2O
-
 Corynebacterium sp. ? + chloride
-
 ?
3.8.1.5 dichloromethane + H2O
-
 Bacillus sp. (in: Bacteria) ? + chloride
-
 ?
3.8.1.5 dichloromethane + H2O
-
 Micrococcus sp. ? + chloride
-
 ?
3.8.1.5 dichloromethane + H2O
-
 Burkholderia sp. ? + chloride
-
 ?
3.8.1.5 monochloroacetic acid + H2O
-
 Pseudomonas sp. glycolate + chloride
-
 ?
3.8.1.5 monochloroacetic acid + H2O
-
 Corynebacterium sp. glycolate + chloride
-
 ?
3.8.1.5 monochloroacetic acid + H2O
-
 Bacillus sp. (in: Bacteria) glycolate + chloride
-
 ?
3.8.1.5 monochloroacetic acid + H2O
-
 Micrococcus sp. glycolate + chloride
-
 ?
3.8.1.5 monochloroacetic acid + H2O
-
 Burkholderia sp. glycolate + chloride
-
 ?
3.8.1.5 tetrachloromethane + H2O
-
 Pseudomonas sp. ? + chloride
-
 ?
3.8.1.5 tetrachloromethane + H2O
-
 Corynebacterium sp. ? + chloride
-
 ?
3.8.1.5 tetrachloromethane + H2O
-
 Bacillus sp. (in: Bacteria) ? + chloride
-
 ?
3.8.1.5 tetrachloromethane + H2O
-
 Micrococcus sp. ? + chloride
-
 ?
3.8.1.5 tetrachloromethane + H2O
-
 Burkholderia sp. ? + chloride
-
 ?
3.8.1.5 trichloroacetic acid + H2O
-
 Pseudomonas sp. ? + chloride
-
 ?
3.8.1.5 trichloroacetic acid + H2O
-
 Corynebacterium sp. ? + chloride
-
 ?
3.8.1.5 trichloroacetic acid + H2O
-
 Bacillus sp. (in: Bacteria) ? + chloride
-
 ?
3.8.1.5 trichloroacetic acid + H2O
-
 Micrococcus sp. ? + chloride
-
 ?
3.8.1.5 trichloroacetic acid + H2O
-
 Burkholderia sp. ? + chloride
-
 ?
3.8.1.5 trichloromethane + H2O
-
 Pseudomonas sp. ? + chloride
-
 ?
3.8.1.5 trichloromethane + H2O
-
 Corynebacterium sp. ? + chloride
-
 ?
3.8.1.5 trichloromethane + H2O
-
 Bacillus sp. (in: Bacteria) ? + chloride
-
 ?
3.8.1.5 trichloromethane + H2O
-
 Micrococcus sp. ? + chloride
-
 ?
3.8.1.5 trichloromethane + H2O
-
 Burkholderia sp. ? + chloride
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.3 9
-
-
 Burkholderia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.3 7.5 10 pH 7.5: about 30% of maximal activity, pH 10.0: about 80% of maximal activity Burkholderia sp.