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Literature summary extracted from

  • Olsson, M.; Duan, R.D.; Ohlsson, L.; Nilsson, A.
    Rat intestinal ceramidase: purification, properties, and physiological relevance (2004), Am. J. Physiol. Gastrointest. Liver Physiol., 287, G929-G937.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate a bile salt analog Rattus norvegicus
3.5.1.23 glycocholic acid
-
 Rattus norvegicus
3.5.1.23 additional information the enzyme is stimulated by bile salts Rattus norvegicus
3.5.1.23 taurocholic acid
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+ over 80% inhibition at 0.2 mM, reversible by EDTA Rattus norvegicus
3.5.1.23 additional information the enzyme is stable to trypsin and chymotrypsin exposure Rattus norvegicus
3.5.1.23 Zn2+ over 90% inhibition at 0.2 mM, reversible by EDTA Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.066
-
 palmitoyl-sphingosine pH 8.0, 37°C Rattus norvegicus
3.5.1.23 0.0714
-
 octanoyl-sphingosine pH 8.0, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 brush border
-
 Rattus norvegicus 5903
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 additional information the enzyme is not affected by Ca2+, Mg2+, or Mn2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 97000
-
 deglycosylated enzyme, gel filtration Rattus norvegicus
3.5.1.23 116000
-
 glycosylated enzyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Rattus norvegicus the enzyme is important in digestion of sphingolipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 native intestinal enzyme over 307fold by acetone precipitation and ion-exchange, hydrophobic-interaction chromatography, and gel filtration, to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 gut lumen Rattus norvegicus
-
3.5.1.23 small intestine mucosa, the enzyme is exposed at the brush border Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 additional information the enzyme is important in digestion of sphingolipids Rattus norvegicus ?
-
 ?
3.5.1.23 octanoyl-sphingosine + H2O
-
 Rattus norvegicus octanoate + sphingosine
-
 ?
3.5.1.23 palmitoyl-sphingosine + H2O
-
 Rattus norvegicus palmitate + sphingosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 neutral ceramidase 2
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 6 8 broad optimum Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 5 9.5 activity range Rattus norvegicus