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Literature summary extracted from
- Purification and characterization of an extracellular lipase from Geotrichum marinum (2004), Lipids, 39, 251-257.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | i.e. CHAPS, activates 3.6fold at 0.1 mM | Geotrichum marinum |  |
| 3.1.1.3 | taurocholic acid | activates 50% at 0.1 mM | Geotrichum marinum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | i.e. CHAPS, 33% inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | cetyltriethylammonium bromide | complete inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | Fe2+ | inhibits at 6 mM | Geotrichum marinum | |
| 3.1.1.3 | methylbenzethonium bromide | 98% inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | Mn2+ | inhibits at 6 mM | Geotrichum marinum | |
| 3.1.1.3 | SDS | complete inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | sodium dioctyl sulfosuccinate | 47% inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | Triton X-100 | complete inhibition at 1 mM | Geotrichum marinum | |
| 3.1.1.3 | Tween 20 | complete inhibition at 1 mM | Geotrichum marinum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | 11.5 | - |
triolein | pH 7.5, 40°C | Geotrichum marinum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.3 | extracellular | - |
Geotrichum marinum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | Ca2+ | stimulates | Geotrichum marinum | |
| 3.1.1.3 | K+ | stimulates at 6 mM, inhibits at 500 mM | Geotrichum marinum | |
| 3.1.1.3 | Mg2+ | stimulates | Geotrichum marinum | |
| 3.1.1.3 | Na+ | stimulates at 6 mM, inhibits at 500 mM | Geotrichum marinum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 62000 | - |
gel filtration | Geotrichum marinum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Geotrichum marinum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | native extracellular enzyme 76fold by affinity chromatography on octyl-resin, and gel filtration | Geotrichum marinum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 773 | - |
purified enzyme | Geotrichum marinum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | substrate specificity, the extracellular enzyme prefers triacylglyceride substrates with cis double bonds in the fatty acid side chains, activity increases with number of double bonds, no activity with tristearin | Geotrichum marinum | ? | - |
? | |
| 3.1.1.3 | trielaidin + H2O | lower activity | Geotrichum marinum | dielaidin + elaidic acid | - |
? | |
| 3.1.1.3 | trilinolein + H2O | good substrate | Geotrichum marinum | dilinolein + linoleate | - |
? | |
| 3.1.1.3 | trilinolenin + H2O | preferred substrate | Geotrichum marinum | dilinolenin + linolenate | - |
? | |
| 3.1.1.3 | triolein + H2O | good substrate | Geotrichum marinum | diolein + oleate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | monomer | 1 * 62000, SDS-PAGE | Geotrichum marinum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | extracellular lipase | - |
Geotrichum marinum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 40 | - |
- |
Geotrichum marinum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 20 | 50 | - |
Geotrichum marinum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 20 | 40 | the purified extracellular enzyme is fully stable for 1 h, but loses 48% and 51% of its activity after 4 h at 20°C and 40°C, respectively | Geotrichum marinum |
| 3.1.1.3 | 60 | - |
inactivation above | Geotrichum marinum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 8 | - |
- |
Geotrichum marinum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 5 | 10 | 20% of maximal activity at pH 5.0, 80% at pH 10.0, inactive at pH 4.0 | Geotrichum marinum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.3 | Geotrichum marinum | isoelectric focusing | - |
4.5 |
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