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Literature summary extracted from

  • Goto, Y.; Hattori, A.; Ishii, Y.; Mizutani, S.; Tsujimoto, M.
    Enzymatic properties of aminopeptidase A: regulation of its enzymatic activity by calcium and angiotensin IV (2006), J. Biol. Chem., 281, 23503-23513.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.7 expression of soluble C-terminally His-tagged enzyme, possessing an N-terminal human trypsin II signal peptide, in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.7 1,10-phenanthroline IC50 is 0.06 mM Homo sapiens
3.4.11.7 amastatin IC50 is 157 nM Homo sapiens
3.4.11.7 angiotensin II competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+ Homo sapiens
3.4.11.7 angiotensin III competitive against Glu-4-methylcoumaryl-7-amide Homo sapiens
3.4.11.7 angiotensin IV competitive feedback inhibition, negative regulatory function in absence or presence of Ca2+; IC50 is 0.010 mM Homo sapiens
3.4.11.7 bradykinin
-
 Homo sapiens
3.4.11.7 Ca2+ inhibits activity with Gln-substrate, regulatory function and influence on substrate specificity Homo sapiens
3.4.11.7 cholecystokinin-8 competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+ Homo sapiens
3.4.11.7 chromogranin A competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+ Homo sapiens
3.4.11.7 E64 slight inhibition at 1 mM Homo sapiens
3.4.11.7 kallidin competitive against Glu-4-methylcoumaryl-7-amide Homo sapiens
3.4.11.7 leupeptin slight inhibition at 1 mM Homo sapiens
3.4.11.7 Met-enkephalin
-
 Homo sapiens
3.4.11.7 Mg2+ inhibits the activity with Glu-substrate moderately Homo sapiens
3.4.11.7 Mn2+ inhibits the activity with Glu-substrate moderately Homo sapiens
3.4.11.7 additional information inhibitory effects of peptide hormones on enzyme activity in presence or absence of Ca2+, overview, no or poor inhibition by 4-(amidinophenyl)-methanesulfonyl fluoride, pepstatin, and bestatin Homo sapiens
3.4.11.7 Neurokinin A
-
 Homo sapiens
3.4.11.7 neurokinin B competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+ Homo sapiens
3.4.11.7 parathyroid hormone residues 69-84
-
 Homo sapiens
3.4.11.7 Substance P
-
 Homo sapiens
3.4.11.7 Zn2+ zinc-metallopeptidase, inhibits the activity with Glu- and Gln-substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.7 additional information
-
 additional information kinetics and substrate specificity in presence or absence of Ca2+ Homo sapiens
3.4.11.7 0.4
-
 Glu-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens
3.4.11.7 0.6
-
 Gln-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 1.2
-
 Glu-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 1.4
-
 Asp-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens
3.4.11.7 2.3
-
 Asp-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 7.1
-
 Gln-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.7 membrane type II membrane-bound enzyme, membrane-spanning Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.7 Ca2+ activates activity with some substrates, decreases activity with other substrates, required for activity with Asp-/Glu-substrates, regulatory function and influence on substrate specificity Homo sapiens
3.4.11.7 Zn2+ zinc-metallopeptidase, inhibits the activity with Glu- and Gln-substrates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.7 angiotensin II + H2O Homo sapiens angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain Asp + angiotensin III
-
 ?
3.4.11.7 cholecystokinin-8 + H2O Homo sapiens i.e. CCK8 ?
-
 ?
3.4.11.7 chromogranin A + H2O Homo sapiens
-
 ?
-
 ?
3.4.11.7 neurokinin B + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.7 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.7 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.7 recombinant soluble enzyme His-tagged from Sf9 insect cells to homogeneity by ultrafiltration, dialysis, anion exchange and cobalt affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.7 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.7 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.7 angiotensin II + H2O angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain Homo sapiens Asp + angiotensin III
-
 ?
3.4.11.7 angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe Homo sapiens Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
3.4.11.7 angiotensin III + H2O i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, low activity in absence of Ca2+ Homo sapiens ? + angiotensin IV
-
 ?
3.4.11.7 Asp-4-methylcoumaryl-7-amide + H2O low activity in absence and presence of Ca2+ Homo sapiens Asp + 7-amino-4-methylcoumarin
-
 ?
3.4.11.7 cholecystokinin-8 + H2O i.e. CCK8 Homo sapiens ?
-
 ?
3.4.11.7 cholecystokinin-8 + H2O i.e. CCK8 or DYMGWMDF-NH2 Homo sapiens Asp + YMGWMDF-NH2
-
 ?
3.4.11.7 chromogranin A + H2O
-
 Homo sapiens ?
-
 ?
3.4.11.7 chromogranin A + H2O i.e. EEEEEMAVVPQGLFRG-NH2 Homo sapiens Glu + EEEEMAVVPQGLFRG-NH2
-
 ?
3.4.11.7 Gln-4-methylcoumaryl-7-amide + H2O low activity in absence and presence of Ca2+ Homo sapiens Gln + 7-amino-4-methylcoumarin
-
 ?
3.4.11.7 Glu-4-methylcoumaryl-7-amide + H2O low activity in absence of Ca2+ Homo sapiens Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.7 kallidin + H2O i.e. Lys-bradykinin or KRPPGFSPFR, the reaction is performed only in absence of Ca2+ Homo sapiens Lys + bradykinin i.e. RPPGFSPFR ?
3.4.11.7 additional information substrate specificity in presence or absence of Ca2+ Homo sapiens ?
-
 ?
3.4.11.7 neurokinin B + H2O
-
 Homo sapiens ?
-
 ?
3.4.11.7 neurokinin B + H2O i.e. DMHDFFVGLM-NH2 Homo sapiens Asp + MHDFFVGLM-NH2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.7 dimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.7 aminopeptidase A
-
 Homo sapiens
3.4.11.7 APA
-
 Homo sapiens
3.4.11.7 More the enzyme belongs to the M1 family Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.7 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.7 2.8
-
 Asp-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 9.5
-
 Gln-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 12.2
-
 Asp-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens
3.4.11.7 13.3
-
 Glu-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ Homo sapiens
3.4.11.7 14.1
-
 Gln-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens
3.4.11.7 33.3
-
 Glu-4-methylcoumaryl-7-amide recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.7 7
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.7 additional information
-
 pH-profile, recombinant enzyme Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.7 0.000157
-
 IC50 is 157 nM Homo sapiens amastatin
3.4.11.7 0.01
-
 IC50 is 0.010 mM Homo sapiens angiotensin IV
3.4.11.7 0.024
-
 competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+ Homo sapiens cholecystokinin-8
3.4.11.7 0.049
-
 competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+ Homo sapiens chromogranin A
3.4.11.7 0.06
-
 competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+ Homo sapiens neurokinin B
3.4.11.7 0.06
-
 IC50 is 0.06 mM Homo sapiens 1,10-phenanthroline
3.4.11.7 0.065
-
 competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+ Homo sapiens angiotensin II