EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.1 | two crystal forms in presence of guanine and phosphate and a third crystal form in presence of xanthine and phosphate, crystals are grown at pH values between 8 and 9, crystals can not be grown at physiologic pH, no good crystals can be obtained in presence of xanthosine, guanosine or ribose 1-phosphate | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.1 | N239D | mutant enzyme shows no activity with the wild-type substrates inosine, xanthosine and guanosine. Unlike the wild-type enzyme, the mutant enzyme shows activity with adenosine | Escherichia coli |
2.4.2.1 | Y191L | mutant enzyme shows no activity with the wild-type substrate xanthosine. The ratio of Vmax to Km for inosine as substrate is 1.7fold lower than the ratio for wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 0.072 | - |
Xanthosine | pH 7.1, 25°C, wild-type enzyme | Escherichia coli | |
2.4.2.1 | 0.155 | - |
guanosine | pH 7.1, 25°C, wild-type enzyme | Escherichia coli | |
2.4.2.1 | 0.231 | - |
Inosine | pH 7.1, 25°C, mutant enzyme Y191L | Escherichia coli | |
2.4.2.1 | 0.249 | - |
guanosine | pH 7.1, 25°C, mutant enzyme Y191L | Escherichia coli | |
2.4.2.1 | 0.262 | - |
adenosine | pH 7.1, 25°C, mutant enzyme N239D | Escherichia coli | |
2.4.2.1 | 0.963 | - |
Inosine | pH 7.1, 25°C, wild-type enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.1 | Escherichia coli | P45563 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | adenosine + phosphate | activity with mutant enzyme N239D, wild-type and mutant enzyme Y191L show no activity | Escherichia coli | alpha-D-ribose 1-phosphate + adenine | - |
? | |
2.4.2.1 | guanosine + phosphate | no activity with mutant enzyme N239D | Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | xanthosine + phosphate | no activity with mutant enzymes Y191L and N239D | Escherichia coli | alpha-D-ribose 1-phosphate + xanthine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.1 | PNP-II | - |
Escherichia coli |
2.4.2.1 | purine nucleoside phosphorylase II | - |
Escherichia coli |