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Literature summary extracted from
- Site-directed mutagenesis experiments on the putative deprotonation site of squalene-hopene cyclase from Alicyclobacillus acidocaldarius (2004), Biosci. Biotechnol. Biochem., 68, 728-738.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | expression in Escherichia coli BL21 | Alicyclobacillus acidocaldarius |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.129 | E45A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | E93A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | F434A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | F437A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | G262A | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | P263A | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | P263G | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | Q262G | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | R127Q | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | T41A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | W133A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | Y267A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45A | mutation located around the "back waters" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E93A | mutation located around the "back waters" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E93A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | F434A | mutation near the substrat channel | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | F434A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | F437A | mutation near the substrat channel | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | F437A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | G262A | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | P263A | mutation located between C29 of the hopanyl cation and the "front water" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | P263A | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | P263G | mutation located between C29 of the hopanyl cation and the "front water" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | P263G | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | Q262A | mutation located between C29 of the hopanyl cation and the "front water" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | Q262G | mutation located between C29 of the hopanyl cation and the "front water" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | Q262G | the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | R127Q | mutation located around the "back waters" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | R127Q | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | T41A | mutation located around the "back waters" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | T41A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | W133A | mutation located around the "back waters" | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | W133A | production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | Y267A | mutation near the substrat channel | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | Y267A | production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures | Alicyclobacillus acidocaldarius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.129 | 0.0162 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius |  |
| 4.2.1.129 | 0.0167 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0169 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0189 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0213 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0255 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0816 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.0955 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.102 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.156 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.185 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.197 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.237 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0162 | - |
squalene | wild-type, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0162 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0167 | - |
squalene | mutant T41A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0167 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0169 | - |
squalene | mutant W133A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0169 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0189 | - |
squalene | mutant E93A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0189 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0213 | - |
squalene | mutant E45A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0213 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0255 | - |
squalene | mutant R127Q, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0255 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0816 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.0955 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.102 | - |
squalene | mutant Q262A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.102 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.156 | - |
squalene | mutant Q262G, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.156 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.185 | - |
squalene | mutant P263A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.185 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.197 | - |
squalene | mutantY267A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.197 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.237 | - |
squalene | mutant P263G, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.237 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.816 | - |
squalene | mutant F434A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.955 | - |
squalene | mutant F437A, 45°C, pH 6.0 | Alicyclobacillus acidocaldarius | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.17 | squalene | Alicyclobacillus acidocaldarius | - |
hop-22(29)-ene | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.129 | Alicyclobacillus acidocaldarius | - |
- |
- |
| 5.4.99.17 | Alicyclobacillus acidocaldarius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | - |
Alicyclobacillus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.129 | hopan-22-ol | - |
Alicyclobacillus acidocaldarius | squalene + H2O | - |
? | |
| 5.4.99.17 | squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? | |
| 5.4.99.17 | squalene | two types of water molecules ("front water" and "back waters") are involved around the deprotonation site. The two residues of Gln262 and Pro263 probably work to keep away the isopropyl group of the hopanyl cation intermediate from the "front water molecule", that is, to place the "front water" in a favorable position, leading to the minimal production of by-products, i.e., hopanol and hop-21(22)-ene. The five residues of Thr41, Glu45, Glu93, Arg127 and Trp133, by which the hydrogen-bonded network incorporating the "back waters" is constructed, increase the polarization of the "front water" to facilitate proton elimination from the isopropyl moiety of the hopanyl cation, leading to the normal product, hop-22(29)-ene | Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.129 | squalene-hopene cyclase | ambiguous | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | SHC | - |
Alicyclobacillus acidocaldarius |
| 5.4.99.17 | squalene-hopene cyclase | - |
Alicyclobacillus acidocaldarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.129 | 35 | 50 | mutant Q262G | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 45 | - |
mutant F437A | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 45 | 55 | mutant Q262A | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 50 | - |
mutant P263G, mutant P263A, mutant E45A, mutant R127Q | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 50 | 60 | mutant Y267A | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 55 | - |
mutant E93A, mutant W133A | Alicyclobacillus acidocaldarius |
| 4.2.1.129 | 60 | - |
wild-type, mutant T41A, mutant F434A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 35 | 50 | mutant Q262G | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 45 | - |
mutant F437A0 | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 45 | - |
mutant F437A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 45 | 55 | mutant Q262A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | - |
mutant E45A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | - |
mutant P263A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | - |
mutant P263G | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | - |
mutant R127Q | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | - |
mutant P263G, mutant P263A, mutant E45A, mutant R127Q | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | 60 | mutantY267A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 50 | 60 | mutant Y267A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 55 | - |
mutant E93A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 55 | - |
mutant W133A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 55 | - |
mutant E93A, mutant W133A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 60 | - |
wild-type | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 60 | - |
mutant F434A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 60 | - |
mutant T41A | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 60 | - |
wild-type, mutant T41A, mutant F434A | Alicyclobacillus acidocaldarius |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.129 | 0.15 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.163 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.163 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.17 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.19 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.21 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.21 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.22 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 0.49 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 2.03 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 3.78 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 3.86 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 4.4 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.345 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.353 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.448 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.54 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.62 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.64 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.735 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 0.86 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 1.03 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 1.06 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 1.09 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 1.21 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 1.37 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.129 | 6 | - |
assay at | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | 6 | - |
assay at | Alicyclobacillus acidocaldarius |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.129 | 1.07 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 1.84 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 5.13 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 8.24 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 8.99 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 9.8 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 10.06 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 11.2 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 16.3 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 19.9 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 20.4 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 20.43 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 4.2.1.129 | 28.2 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 3.2 | - |
squalene | pH 6.0, 45°C, mutant Y267A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 4.5 | - |
squalene | pH 6.0, 45°C, mutant P263G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 5.9 | - |
squalene | pH 6.0, 45°C, mutant P263A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 6.6 | - |
squalene | pH 6.0, 45°C, mutant F434A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 8.8 | - |
squalene | pH 6.0, 45°C, mutant Q262G | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 10.8 | - |
squalene | pH 6.0, 45°C, mutant F437A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 11.9 | - |
squalene | pH 6.0, 45°C, mutant Q262A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 16.1 | - |
squalene | pH 6.0, 45°C, mutant E45A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 17.6 | - |
squalene | pH 6.0, 45°C, mutant R127Q | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 18.7 | - |
squalene | pH 6.0, 45°C, mutant E93A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 36.7 | - |
squalene | pH 6.0, 45°C, mutant W133A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 44 | - |
squalene | pH 6.0, 45°C, mutant T41A | Alicyclobacillus acidocaldarius | |
| 5.4.99.17 | 53.09 | - |
squalene | pH 6.0, 45°C, wild-type enzyme | Alicyclobacillus acidocaldarius | |
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