Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, Y.; Yao, Z.X.; Papadopoulos, V.
    Cytochrome P450 17alpha hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells (2005), Mol. Endocrinol., 19, 1918-1931.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.17 expression of His-tagged cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.17 additional information knockout of cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 shows dramatically reduced de novo synthesis of steroids, e.g. progesterone, which can partially be rescued by transfection of CYP17, the latter cells can synthesize progesterone if supplemented with precusors squalene epoxide, lanosterol, zymosterol, and desmosterol, but not squalene Mus musculus
1.14.14.19 additional information 17alphahydroxylase/17,20-lyase knock-down clone shows dramatically reducued human chorionic gonadotropin-induced progesterone formation and de novo synthesis of steroids. Addition of squalene epoxide, lanosterol, zymosterol, and desmosterol can rescue the hormone-induced progesterone formation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.17 additional information inhibitor SU10603 inhibits the cytochrome P450 17alpha hydroxylase/17,20 lyase activity of CYP17 but not the squalene epoxidase activity Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.17 microsome
-
 Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.17 additional information Mus musculus the enzyme activity is associated with cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in cholesterol biosynthesis in Leydig cells ?
-
 ?
1.14.14.17 squalene + reduced acceptor + O2 Mus musculus epoxidation of squalene is the first step in cholesterol biosynthesis (S)-squalene-2,3-epoxide + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.17 Mus musculus
-
-
-
1.14.14.19 Mus musculus
-
 in addition to 17alphahydroxylase/17,20-lyase activity, enzyme can also act as squalene epoxidase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.17 squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 also catalyzes the reaction of squalene epoxidase Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.17 Leydig cell tumor cell defective in cytochrome P450 17alpha hydroxylase/17,20 lyase activity Mus musculus
-
1.14.14.19 MA-10 cell mouse tumor leydig cell defective in 17alphahydroxylase/17,20-lyase activity Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.17 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.17 additional information the enzyme activity is associated with cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17 in cholesterol biosynthesis in Leydig cells Mus musculus ?
-
 ?
1.14.14.17 squalene + reduced acceptor + O2
-
 Mus musculus (S)-squalene-2,3-epoxide + acceptor + H2O
-
 ?
1.14.14.17 squalene + reduced acceptor + O2 epoxidation of squalene is the first step in cholesterol biosynthesis Mus musculus (S)-squalene-2,3-epoxide + acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.17 ? x * 48000-50000 + x * 28000-30000, recombinant His-tagged cytochrome P450 17alpha hydroxylase/17,20 lyase CYP17, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.17 CYP17
-
 Mus musculus
1.14.14.17 cytochrome P450 17alpha hydroxylase/17,20 lyase
-
 Mus musculus
1.14.14.17 squalene epoxidase
-
 Mus musculus