Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cacciapuoti, G.; Forte, S.; Moretti, M.A.; Brio, A.; Zappia, V.; Porcelli, M.
    A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus (2005), FEBS J., 272, 1886-1899.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 overexpression in Escherichia coli BL21 Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.28 C259S/C261S mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme Saccharolobus solfataricus
2.4.2.28 C262S mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.0254
-
adenosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 0.084
-
Inosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 0.1136
-
guanosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 0.154
-
5'-methylthioadenosine pH 7.4, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 30000
-
6 * 30000, SDS-PAGE Saccharolobus solfataricus
2.4.2.28 180000
-
gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
?
2.4.2.28 adenosine + phosphate
-
Saccharolobus solfataricus adenine + D-ribose 1-phosphate
-
?
2.4.2.28 guanosine + phosphate
-
Saccharolobus solfataricus guanine + D-ribose 1-phosphate
-
?
2.4.2.28 inosine + phosphate
-
Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 hexamer 6 * 30000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 5'-deoxy-5'-methylthioadenosine phosphorylase II
-
Saccharolobus solfataricus
2.4.2.28 SsMTAPII
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 120
-
wild-type enzyme Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.28 95 140 95°C: about 50% of maximal activity, 140°C: about 45% of maximal activity, wild-type enzyme Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 90
-
1 h, wild-type enzyme is completely stable, mutant enzyme C262S loses 32% of its activity, mutant enzyme c259S/C261A loses 63% of its activity Saccharolobus solfataricus
2.4.2.28 102
-
apparent Tm, mutant enzyme C259S/C261S Saccharolobus solfataricus
2.4.2.28 106
-
apparent Tm, mutant enzyme C262S Saccharolobus solfataricus
2.4.2.28 112
-
apparent Tm, wild-type enzyme Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.28 21.4
-
5'-methylthioadenosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 29.6
-
guanosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 43.6
-
adenosine pH 7.4, 70°C Saccharolobus solfataricus
2.4.2.28 132
-
Inosine pH 7.4, 70°C Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.28 Saccharolobus solfataricus calculation from nucleotide sequence
-
6.54