EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.28 | overexpression in Escherichia coli BL21 | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.28 | C259S/C261S | mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme | Saccharolobus solfataricus |
2.4.2.28 | C262S | mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.28 | 0.0254 | - |
adenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 0.084 | - |
Inosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 0.1136 | - |
guanosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 0.154 | - |
5'-methylthioadenosine | pH 7.4, 70°C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 30000 | - |
6 * 30000, SDS-PAGE | Saccharolobus solfataricus |
2.4.2.28 | 180000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.28 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.28 | 5'-methylthioadenosine + phosphate | - |
Saccharolobus solfataricus | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? | |
2.4.2.28 | adenosine + phosphate | - |
Saccharolobus solfataricus | adenine + D-ribose 1-phosphate | - |
? | |
2.4.2.28 | guanosine + phosphate | - |
Saccharolobus solfataricus | guanine + D-ribose 1-phosphate | - |
? | |
2.4.2.28 | inosine + phosphate | - |
Saccharolobus solfataricus | hypoxanthine + D-ribose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.28 | hexamer | 6 * 30000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.28 | 5'-deoxy-5'-methylthioadenosine phosphorylase II | - |
Saccharolobus solfataricus |
2.4.2.28 | SsMTAPII | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 120 | - |
wild-type enzyme | Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 95 | 140 | 95°C: about 50% of maximal activity, 140°C: about 45% of maximal activity, wild-type enzyme | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 90 | - |
1 h, wild-type enzyme is completely stable, mutant enzyme C262S loses 32% of its activity, mutant enzyme c259S/C261A loses 63% of its activity | Saccharolobus solfataricus |
2.4.2.28 | 102 | - |
apparent Tm, mutant enzyme C259S/C261S | Saccharolobus solfataricus |
2.4.2.28 | 106 | - |
apparent Tm, mutant enzyme C262S | Saccharolobus solfataricus |
2.4.2.28 | 112 | - |
apparent Tm, wild-type enzyme | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.28 | 21.4 | - |
5'-methylthioadenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 29.6 | - |
guanosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 43.6 | - |
adenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
2.4.2.28 | 132 | - |
Inosine | pH 7.4, 70°C | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.4.2.28 | Saccharolobus solfataricus | calculation from nucleotide sequence | - |
6.54 |