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Literature summary extracted from

  • Versees, W.; Decanniere, K.; Pelle, R.; Depoorter, J.; Brosens, E.; Parkin, D.W.; Steyaert, J.
    Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax (2001), J. Mol. Biol., 307, 1363-1379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.1
-
 Trypanosoma vivax

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.1 hanging drop vapor diffusion method, unliganded structure and 3-deaza-adenosine complex Trypanosoma vivax

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 3-deazaadenosine
-
 Trypanosoma vivax
3.2.2.1 7-deazaadenosine
-
 Trypanosoma vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.00382
-
 guanosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.0052
-
 Inosine pH 7.2, 35°C, without His-tag Trypanosoma vivax
3.2.2.1 0.00537
-
 Inosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.00854
-
 adenosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.586
-
 uridine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.925
-
 cytidine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 257
-
 p-nitrophenylriboside pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Trypanosoma vivax Q9GPQ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Trypanosoma vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 adenosine + H2O
-
 Trypanosoma vivax adenine + D-ribose
-
 ?
3.2.2.1 cytidine + H2O
-
 Trypanosoma vivax cytosine + D-ribose
-
 ?
3.2.2.1 guanosine + H2O
-
 Trypanosoma vivax guanine + D-ribose
-
 ?
3.2.2.1 inosine + H2O
-
 Trypanosoma vivax hypoxanthine + D-ribose
-
 ?
3.2.2.1 p-nitrophenylriboside + H2O
-
 Trypanosoma vivax p-nitrophenol + D-ribose
-
 ?
3.2.2.1 uridine + H2O
-
 Trypanosoma vivax uracil + D-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.1 IAG-NH
-
 Trypanosoma vivax
3.2.2.1 inosine-adenosine-guanosine-preferring nucleoside hydrolase
-
 Trypanosoma vivax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.1 0.022
-
 uridine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.206
-
 p-nitrophenylriboside pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.338
-
 cytidine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 1.46
-
 adenosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 1.9
-
 guanosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 5.19
-
 Inosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 5.49
-
 Inosine pH 7.2, 35°C, wiithout His-tag Trypanosoma vivax

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.1 0.0002
-
 3-deazaadenosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax
3.2.2.1 0.3565
-
 7-deazaadenosine pH 7.2, 35°C, His-tagged enzyme form Trypanosoma vivax