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Literature summary extracted from

  • Alber, B.E.; Fuchs, G.
    Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation (2002), J. Biol. Chem., 277, 12137-12143.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.17 0.01
-
 NADPH pH 7.8, 55°C Chloroflexus aurantiacus
6.2.1.17 0.01
-
 CoA pH 7.8, 55°C Chloroflexus aurantiacus
6.2.1.17 0.015
-
 3-hydroxypropionate pH 7.8, 55°C Chloroflexus aurantiacus
6.2.1.17 0.05
-
 ATP pH 7.8, 55°C Chloroflexus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.17 Cs+ can partially substitute for K+ Chloroflexus aurantiacus
6.2.1.17 K+ required, half aximal activity at 4 mM Chloroflexus aurantiacus
6.2.1.17 Mg2+ required Chloroflexus aurantiacus
6.2.1.17 NH4+ can partially substitute for K+ Chloroflexus aurantiacus
6.2.1.17 Rb+ can partially substitute for K+ Chloroflexus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.17 185000
-
 SDS-PAGE Chloroflexus aurantiacus
6.2.1.17 500000 800000 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH Chloroflexus aurantiacus involved in CO2 fixation via 3-hydroxypropionate cycle AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH Chloroflexus aurantiacus OK-70-fl / DSM 636 involved in CO2 fixation via 3-hydroxypropionate cycle AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.17 Chloroflexus aurantiacus Q8VRG6
-
-
6.2.1.17 Chloroflexus aurantiacus OK-70-fl / DSM 636 Q8VRG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.17
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.17 2.5
-
 purified enzyme, pH 7.8, 55°C Chloroflexus aurantiacus

Storage Stability

EC Number Storage Stability Organism
6.2.1.17 -20°C, 20% glycerol, 1 mM dithioerythreitol, stable for several weeks Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH involved in CO2 fixation via 3-hydroxypropionate cycle Chloroflexus aurantiacus AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates Chloroflexus aurantiacus AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH involved in CO2 fixation via 3-hydroxypropionate cycle Chloroflexus aurantiacus OK-70-fl / DSM 636 AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + 3-hydroxypropionate + CoA + NADPH three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates Chloroflexus aurantiacus OK-70-fl / DSM 636 AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA + NADPH same activity as with 3-hydroxypropionate Chloroflexus aurantiacus AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA + NADPH same activity as with 3-hydroxypropionate Chloroflexus aurantiacus OK-70-fl / DSM 636 AMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 GTP + 3-hydroxypropionate + CoA + NADPH 24% of activity Chloroflexus aurantiacus GMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 GTP + 3-hydroxypropionate + CoA + NADPH 24% of activity Chloroflexus aurantiacus OK-70-fl / DSM 636 GMP + diphosphate + NADP+ + propionyl-CoA
-
 ?
6.2.1.17 additional information no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate Chloroflexus aurantiacus ?
-
 ?
6.2.1.17 additional information no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate Chloroflexus aurantiacus OK-70-fl / DSM 636 ?
-
 ?
6.2.1.17 UTP + 3-hydroxypropionate + CoA + NADPH 20% of activity Chloroflexus aurantiacus UMP + diphosphate + NADP+ + propionyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.17 More homotrimer or homotetramer Chloroflexus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.17 65
-
 stable for at least 15 min in presence of 20% glycerol and 1 mM dithioerythreitol Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.17 12
-
 3-hydroxypropionate pH 7.8, 55°C Chloroflexus aurantiacus