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Literature summary extracted from

  • Ogawa, J.; Sulistyaningdyah, W.T.; Li, Q.S.; Tanaka, H.; Xie, S.X.; Kano, K.; Ikeda, T.; Shimizu, S.
    Two extracellular proteins with alkaline peroxidase activity, a novel cytochrome c and a catalase-peroxidase, from Bacillus sp. No.13 (2004), Biochim. Biophys. Acta, 1699, 65-75.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 4-aminoantipyrine inhibition of catalase activity Bacillus sp. (in: Bacteria)
1.11.1.6 phenol inhibition of catalase activity Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 0.67
-
 H2O2 catalase activity, pH 8.5, 30°C Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.6 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 51000
-
 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)
1.11.1.6 204000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Bacillus sp. (in: Bacteria)
-
 O2 + H2O
-
 ?
1.11.1.6 H2O2 Bacillus sp. (in: Bacteria) 13
-
 O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Bacillus sp. (in: Bacteria)
-
-
-
1.11.1.6 Bacillus sp. (in: Bacteria) 13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 3.8fold from cell culture supernatant Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 0.94
-
 purified enzyme, peroxidase activity, mixed-type Bacillus sp. (in: Bacteria)
1.11.1.6 55000
-
 purified enzyme, catalase activity, mixed-type Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Bacillus sp. (in: Bacteria) O2 + H2O
-
 ?
1.11.1.6 H2O2
-
 Bacillus sp. (in: Bacteria) 13 O2 + H2O
-
 ?
1.11.1.6 H2O2 + 2-aminophenol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + 2-aminophenol
-
 Bacillus sp. (in: Bacteria) 13 ?
-
 ?
1.11.1.6 H2O2 + 3-chlorophenol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + 3-chlorophenol
-
 Bacillus sp. (in: Bacteria) 13 ?
-
 ?
1.11.1.6 H2O2 + 4-chlorophenol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + 4-chlorophenol
-
 Bacillus sp. (in: Bacteria) 13 ?
-
 ?
1.11.1.6 H2O2 + 4-nitrophenol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + dimethylaniline low activity Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + phenol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + propyl gallate best peroxidase substrate Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 H2O2 + pyrocatechol
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 additional information enzyme also shows alkaline peroxidase activity, which is much lower than the catalase activity, no peroxidase activity with 3-aminophenol, 2,4,6-trichlorophenol, and 3-nitrophenol Bacillus sp. (in: Bacteria) ?
-
 ?
1.11.1.6 additional information enzyme also shows alkaline peroxidase activity, which is much lower than the catalase activity, no peroxidase activity with 3-aminophenol, 2,4,6-trichlorophenol, and 3-nitrophenol Bacillus sp. (in: Bacteria) 13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 catalase-peroxidase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 30
-
 catalase activity Bacillus sp. (in: Bacteria)
1.11.1.6 60
-
 peroxidase activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 30
-
 85% remaining peroxidase activity after 20 h at pH 9.0 Bacillus sp. (in: Bacteria)
1.11.1.6 60
-
 pH 8.5, stable up to, catalase and peroxidase activities Bacillus sp. (in: Bacteria)
1.11.1.6 70
-
 70% decreased activity Bacillus sp. (in: Bacteria)
1.11.1.6 80
-
 95% decreased activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7 10 peroxidase and catalase activities Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 6 11 over 80% remaining activity after 1 h at 30°C Bacillus sp. (in: Bacteria)
1.11.1.6 9
-
 85% remaining activity after 20 h at 30°C Bacillus sp. (in: Bacteria)

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.6 0.053
-
 4-aminoantipyrine catalase activity, pH 8.5, 30°C Bacillus sp. (in: Bacteria)
1.11.1.6 0.14
-
 phenol catalase activity, pH 8.5, 30°C Bacillus sp. (in: Bacteria)