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Literature summary extracted from

  • Larroy, C.; Fernandez, M.R.; Gonzalez, E.; Pares, X.; Biosca, J.A.
    Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reduction (2002), Biochem. J., 361, 163-172.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.2 additional information gene deletion mutant and combined gene deletion mutant lacking both ADH and ADH7, both are viable and show similar growth curves as wild-type Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.011
-
 NADPH pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.013
-
 NADP+ pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.029
-
 NADPH pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.043
-
 cinnamaldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.048
-
 3-Methylbutanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.049
-
 pentanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.058
-
 veratraldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.06
-
 pentanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.061
-
 NADP+ pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.073
-
 veratraldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.08
-
 cinnamyl alcohol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.11
-
 cinnamyl alcohol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.129
-
 3-Methylbutanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.152
-
 hexanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.172
-
 cinnamaldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.436
-
 cinnamyl alcohol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.7
-
 cinnamyl alcohol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 0.99
-
 1-Pentanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 1.61
-
 3-methyl-1-butanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 2.2
-
 Phenylethanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 4.6
-
 3-methyl-1-butanol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 9.1
-
 Hexanol pH 7.0, 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 40000
-
 2 * 40000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.2 71000
-
 gel filtration Saccharomyces cerevisiae
1.1.1.2 81000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Saccharomyces cerevisiae
-
-
-
1.1.1.2 Saccharomyces cerevisiae
-
 ADH7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 90
-
 pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 183
-
 25°C, pH 7.0 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 -20°C, 20% v/v glycerol, stable for at least one month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (2E)-4-hydroxynon-2-enal + NADPH 30% of activity with cinnamaldehyde Saccharomyces cerevisiae (2E)-non-2-ene-1,4-diol + NADP+
-
 r
1.1.1.2 (2S)-methyl-1-butanol + NADP+ 39% activity compared to cinnamyl alcohol Saccharomyces cerevisiae (2S)-methylbutanal + NADPH
-
 r
1.1.1.2 1-butanol + NADP+ 42% activity compared to cinnamyl alcohol Saccharomyces cerevisiae butanal + NADPH
-
 r
1.1.1.2 1-hexanol + NADP+ 45% activity compared to cinnamyl alcohol Saccharomyces cerevisiae hexanal + NADPH
-
 r
1.1.1.2 1-octanol + NADP+ 30% activity compared to cinnamyl alcohol Saccharomyces cerevisiae octanal + NADPH
-
 r
1.1.1.2 1-pentanol + NADP+ 46% activity compared to cinnamyl alcohol Saccharomyces cerevisiae pentanal + NADPH
-
 r
1.1.1.2 1-propanol + NADP+ 16% activity compared to cinnamyl alcohol Saccharomyces cerevisiae propanal + NADPH
-
 r
1.1.1.2 2-(S)-methylbutanol + NADP+ 7% of activity with cinnamyl alcohol Saccharomyces cerevisiae (2S)-2-methylbutanal + NADPH
-
 r
1.1.1.2 2-butanol + NADP+ 2% of activity with cinnamyl alcohol Saccharomyces cerevisiae 2-butanone + NADPH
-
 r
1.1.1.2 2-butanol + NADP+ 1% activity compared to cinnamyl alcohol Saccharomyces cerevisiae butanone + NADPH
-
 r
1.1.1.2 2-methyl-1-propanol + NADP+ 28% activity compared to cinnamyl alcohol Saccharomyces cerevisiae 2-methylpropanal + NADPH
-
 r
1.1.1.2 2-methylbutanal + NADPH 89% activity compared to cinnamaldehyde Saccharomyces cerevisiae 2-methyl-1-butanol + NADP+
-
 r
1.1.1.2 2-methylbutanal + NADPH 92% of activity with cinnamaldehyde Saccharomyces cerevisiae 2-methyl-1-butanol + NADP+
-
 r
1.1.1.2 2-methylpropanal + NADPH 63% activity compared to cinnamaldehyde Saccharomyces cerevisiae 2-methyl-1-propanol + NADP+
-
 r
1.1.1.2 2-methylpropanal + NADPH 67% of activity with cinnamaldehyde Saccharomyces cerevisiae 2-methyl-1-propanol + NADP+
-
 r
1.1.1.2 2-methylpropanol + NADP+ 1% of activity with cinnamyl alcohol Saccharomyces cerevisiae 2-methylpropanal + NADPH
-
 r
1.1.1.2 2-phenylethanol + NADP+ 23% activity compared to cinnamyl alcohol Saccharomyces cerevisiae phenylacetaldehyde + NADPH
-
 r
1.1.1.2 3-methyl-1-butanol + NADP+ 40% activity compared to cinnamyl alcohol Saccharomyces cerevisiae 3-methylbutanal + NADPH
-
 r
1.1.1.2 3-methylbutanal + NADPH 79% activity compared to cinnamaldehyde Saccharomyces cerevisiae 3-methyl-1-butanol + NADP+
-
 r
1.1.1.2 3-methylbutanal + NADPH + H+ 84% of activity with cinnamaldehyde Saccharomyces cerevisiae 3-methyl-1-butanol + NADP+
-
 r
1.1.1.2 3-methylbutanol + NADP+ 15% of activity with cinnamyl alcohol Saccharomyces cerevisiae 3-methylbutanal + NADPH
-
 r
1.1.1.2 anisaldehyde + NADPH 72% of activity with cinnamaldehyde Saccharomyces cerevisiae 4-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.2 anisaldehyde + NADPH 83% activity compared to cinnamaldehyde Saccharomyces cerevisiae 4-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.2 benzaldehyde + NADPH 45% activity compared to cinnamaldehyde Saccharomyces cerevisiae benzyl alcohol + NADP+
-
 r
1.1.1.2 benzaldehyde + NADPH 72% of activity with cinnamaldehyde Saccharomyces cerevisiae benzyl alcohol + NADP+
-
 r
1.1.1.2 benzyl alcohol + NADP+ 45% of activity with cinnamyl alcohol Saccharomyces cerevisiae benzaldehyde + NADPH
-
 r
1.1.1.2 benzyl alcohol + NADP+ 78% activity compared to cinnamyl alcohol Saccharomyces cerevisiae benzaldehyde + NADPH
-
 r
1.1.1.2 butanal + NADPH + H+ 19% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-butanol + NADP+
-
 r
1.1.1.2 butanal + NADPH + H+ 57% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-butanol + NADP+
-
 r
1.1.1.2 butane-1,2-diol + NADP+ 2% activity compared to cinnamyl alcohol Saccharomyces cerevisiae butanal-2-ol + NADPH
-
 r
1.1.1.2 butane-1,3-diol + NADP+ 5% activity compared to cinnamyl alcohol Saccharomyces cerevisiae butanal-3-ol + NADPH
-
 r
1.1.1.2 butane-1,4-diol + NADP+ 8% activity compared to cinnamyl alcohol Saccharomyces cerevisiae butanal-4-ol + NADPH
-
 r
1.1.1.2 butanol + NADP+ 24% of activity with cinnamyl alcohol Saccharomyces cerevisiae butanal + NADPH
-
 r
1.1.1.2 cinnamaldehyde + NADPH
-
 Saccharomyces cerevisiae cinnamyl alcohol + NADP+
-
 r
1.1.1.2 cinnamyl alcohol + NADP+
-
 Saccharomyces cerevisiae cinnamaldehyde + NADPH
-
 r
1.1.1.2 coniferaldehyde + NADPH 10% of activity with cinnamaldehyde Saccharomyces cerevisiae coniferyl alcohol + NADP+
-
 r
1.1.1.2 coniferaldehyde + NADPH 15% activity compared to cinnamaldehyde Saccharomyces cerevisiae coniferyl alcohol + NADP+
-
 r
1.1.1.2 coniferyl alcohol + NADP+ 27% of activity with cinnamyl alcohol Saccharomyces cerevisiae coniferylaldehyde + NADPH + H+
-
 r
1.1.1.2 coniferyl alcohol + NADP+ 7% activity compared to cinnamyl alcohol Saccharomyces cerevisiae coniferaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+ 2% activity compared to cinnamyl alcohol Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+ 4% of activity with cinnamyl alcohol Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
 r
1.1.1.2 furfural + NADPH 27% of activity with cinnamaldehyde Saccharomyces cerevisiae furfuryl alcohol + NADP+
-
 r
1.1.1.2 furfural + NADPH 54% activity compared to cinnamaldehyde Saccharomyces cerevisiae furfuryl alcohol + NADP+
-
 r
1.1.1.2 heptanal + NADPH 86% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-heptanol + NADP+
-
 r
1.1.1.2 heptanal + NADPH 88% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-heptanol + NADP+
-
 r
1.1.1.2 hexanal + NADPH + H+ 88% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-hexanol + NADP+
-
 r
1.1.1.2 hexanal + NADPH + H+ 89% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-hexanol + NADP+
-
 r
1.1.1.2 hexanol + NADP+ 154% of activity with cinnamyl alcohol Saccharomyces cerevisiae hexanal + NADPH
-
 r
1.1.1.2 additional information no subbstrate: acetone, methylglyoxal, cyclohexanone Saccharomyces cerevisiae ?
-
 ?
1.1.1.2 octanal + NADPH + H+ 59% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-octanol + NADP+
-
 r
1.1.1.2 octanal + NADPH + H+ 84% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-octanol + NADP+
-
 r
1.1.1.2 octanol + NADP+ 52% of activity with cinnamyl alcohol Saccharomyces cerevisiae octanal + NADPH
-
 r
1.1.1.2 pentanal + NADPH 109% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-pentanol + NADP+
-
 r
1.1.1.2 pentanal + NADPH 153% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-pentanol + NADP+
-
 r
1.1.1.2 pentanol + NADP+ 89% of activity with cinnamyl alcohol Saccharomyces cerevisiae pentanal + NADPH
-
 r
1.1.1.2 phenylacetaldehyde + NADPH + H+ 90% activity compared to cinnamaldehyde Saccharomyces cerevisiae 2-phenylethanol + NADP+
-
 r
1.1.1.2 propanal + NADPH + H+ 21% of activity with cinnamaldehyde Saccharomyces cerevisiae 1-propanol + NADP+
-
 r
1.1.1.2 propanal + NADPH + H+ 24% activity compared to cinnamaldehyde Saccharomyces cerevisiae 1-propanol + NADP+
-
 r
1.1.1.2 propanol + NADP+ 7% of activity with cinnamyl alcohol Saccharomyces cerevisiae propanal + NADPH
-
 r
1.1.1.2 sinapaldehyde + NADPH + H+ 3% of activity with cinnamaldehyde Saccharomyces cerevisiae sinapyl alcohol + NADP+
-
 r
1.1.1.2 trans-2-nonenal + NADPH 33% activity compared to cinnamaldehyde Saccharomyces cerevisiae trans-2-nonen-1-ol + NADP+
-
 r
1.1.1.2 trans-2-nonenal + NADPH 39% of activity with cinnamaldehyde Saccharomyces cerevisiae trans-2-nonenol + NADP+
-
 r
1.1.1.2 vanillin + NADPH 57% of activity with cinnamaldehyde Saccharomyces cerevisiae 4-hydroxy-3-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.2 vanillin + NADPH 84% activity compared to cinnamaldehyde Saccharomyces cerevisiae 4-hydroxy-3-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.2 veratraldehyde + NADPH 79% activity compared to cinnamaldehyde Saccharomyces cerevisiae 3,4-dimethoxybenzyl alcohol + NADP+
-
 r
1.1.1.2 veratraldehyde + NADPH 82% of activity with cinnamaldehyde Saccharomyces cerevisiae 3,4-dimethoxybenzyl alcohol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer 2 * 40000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 10.7
-
 Hexanol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 14.8
-
 cinnamyl alcohol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 70
-
 cinnamyl alcohol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 173
-
 3-methyl-1-butanol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 263
-
 veratraldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 296
-
 3-Methylbutanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 307
-
 cinnamaldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 359
-
 hexanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 378
-
 pentanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 918
-
 1-Pentanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 1132
-
 Phenylethanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 1622
-
 cinnamyl alcohol pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 2866
-
 3-methyl-1-butanol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 3404
-
 cinnamyl alcohol pH 10.0, 25°C Saccharomyces cerevisiae
1.1.1.2 6000
-
 veratraldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 7913
-
 cinnamaldehyde pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 9581
-
 3-Methylbutanal pH 7.0, 25°C Saccharomyces cerevisiae
1.1.1.2 11920
-
 pentanal pH 7.0, 25°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
 optimum for reduction reaction, assay of reduction and oxidation at Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.2 6 11 oxidation reaction, increasing activity from pH 6.0 to 11.0 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.2 Saccharomyces cerevisiae isoelectric focusing
-
 4.9
1.1.1.2 Saccharomyces cerevisiae and 6.2, isoelectric focusing
-
 6
1.1.1.2 Saccharomyces cerevisiae and 6.4, isoelectric focusing
-
 6