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Literature summary extracted from

  • Cristea, M.; Engstrom, K.; Su, C.; Hornsten, L.; Oliw, E.H.
    Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands (2005), Arch. Biochem. Biophys., 434, 201-211.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.45 H274Q no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
1.13.11.45 H278E no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
1.13.11.45 H462E no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
1.13.11.45 H463Q no significant change in activity Gaeumannomyces graminis
1.13.11.45 additional information V602 deletion mutant, no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
1.13.11.45 N466L no significant change in activity Gaeumannomyces graminis
1.13.11.45 N466Q no significant change in activity Gaeumannomyces graminis
1.13.11.45 Q467N no significant change in activity Gaeumannomyces graminis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.45 0.0071
-
 alpha-linolenic acid pH 9.0, 21°C Gaeumannomyces graminis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.45 Mn2+ 1 mol per mol of protein Gaeumannomyces graminis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.45 Gaeumannomyces graminis
-
 expression in Pichia pastoris
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.45 glycoprotein N-and O-glycosilation Gaeumannomyces graminis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.45 culture supernatant
-
 Gaeumannomyces graminis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.45 18
-
 pH 9.0, 21°C Gaeumannomyces graminis

Storage Stability

EC Number Storage Stability Organism
1.13.11.45 4°C, stable for months Gaeumannomyces graminis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.45 alpha-linolenic acid + O2
-
 Gaeumannomyces graminis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.45 ? x * 80-100000, SDS-PAGE Gaeumannomyces graminis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.45 9
-
-
 Gaeumannomyces graminis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.45 7
-
 93% of maximum activity Gaeumannomyces graminis
1.13.11.45 8
-
 98% of maximum activity Gaeumannomyces graminis