EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.61 | functional expression in hairy root cells of Datura stramonium via infection with Agrobacterium rhizogenes, no accumulation of 4-hydroxybenzaldehyde derivatives occurs, but diminished feruloyl-CoA availability, leading to reduced lignin formation, and formation of new glucoconjugates of 4-hydroxybenzaldehyde derivatives, e.g. 4-hydroxybenzyl alcohol-O-beta-D-glucoside | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.61 | 31000 | - |
x * 31000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.61 | 4-coumaroyl-CoA | Pseudomonas fluorescens | i.e. 4-hydroxycinnamoyl-CoA | 4-hydroxybenzaldehyde + acetyl-CoA | - |
ir | |
4.1.2.61 | 4-coumaroyl-CoA | Pseudomonas fluorescens AN103 | i.e. 4-hydroxycinnamoyl-CoA | 4-hydroxybenzaldehyde + acetyl-CoA | - |
ir | |
4.1.2.61 | 4-feruloyl-CoA | Pseudomonas fluorescens | i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | i.e. vanillin | ir | |
4.1.2.61 | caffeoyl-CoA | Pseudomonas fluorescens | i.e. 3,4-dihydroxycinnamoyl-CoA | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ir | |
4.1.2.61 | caffeoyl-CoA | Pseudomonas fluorescens AN103 | i.e. 3,4-dihydroxycinnamoyl-CoA | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.61 | Pseudomonas fluorescens | - |
- |
- |
4.1.2.61 | Pseudomonas fluorescens AN103 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.61 | - |
Pseudomonas fluorescens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.61 | additional information | - |
enzyme activity in several transgenic cell lines from hairy roots of Datura stramonium | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.61 | 4-coumaroyl-CoA | i.e. 4-hydroxycinnamoyl-CoA | Pseudomonas fluorescens | 4-hydroxybenzaldehyde + acetyl-CoA | - |
? | |
4.1.2.61 | 4-coumaroyl-CoA | i.e. 4-hydroxycinnamoyl-CoA | Pseudomonas fluorescens | 4-hydroxybenzaldehyde + acetyl-CoA | - |
ir | |
4.1.2.61 | 4-coumaroyl-CoA | i.e. 4-hydroxycinnamoyl-CoA | Pseudomonas fluorescens AN103 | 4-hydroxybenzaldehyde + acetyl-CoA | - |
? | |
4.1.2.61 | 4-coumaroyl-CoA | i.e. 4-hydroxycinnamoyl-CoA | Pseudomonas fluorescens AN103 | 4-hydroxybenzaldehyde + acetyl-CoA | - |
ir | |
4.1.2.61 | 4-feruloyl-CoA | i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | Pseudomonas fluorescens | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | i.e. vanillin | ir | |
4.1.2.61 | 4-trans-feruloyl-CoA | i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | Pseudomonas fluorescens | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | i.e. vanillin | ? | |
4.1.2.61 | 4-trans-feruloyl-CoA | i.e. 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA | Pseudomonas fluorescens AN103 | 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA | i.e. vanillin | ? | |
4.1.2.61 | caffeoyl-CoA | i.e. 3,4-dihydroxycinnamoyl-CoA | Pseudomonas fluorescens | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ? | |
4.1.2.61 | caffeoyl-CoA | i.e. 3,4-dihydroxycinnamoyl-CoA | Pseudomonas fluorescens | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ir | |
4.1.2.61 | caffeoyl-CoA | i.e. 3,4-dihydroxycinnamoyl-CoA | Pseudomonas fluorescens AN103 | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ? | |
4.1.2.61 | caffeoyl-CoA | i.e. 3,4-dihydroxycinnamoyl-CoA | Pseudomonas fluorescens AN103 | 3,4-dihydroxybenzaldehyde + acetyl-CoA | i.e. protocatechuic aldehyde | ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.61 | ? | x * 31000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.61 | 4-hydroxycinnamoyl-CoA hydratase/lyase | - |
Pseudomonas fluorescens |
4.1.2.61 | HCHL | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.61 | 30 | - |
assay at | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.61 | 8.5 | - |
assay at | Pseudomonas fluorescens |