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Literature summary extracted from
- A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane (2001), Plant Physiol., 127, 1102-1112.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | linoleic acid | less than oleic acid | Arabidopsis thaliana |  |
| 3.1.4.4 | linolenic acid | less than oleic acid | Arabidopsis thaliana | |
| 3.1.4.4 | additional information | no activation by stearic or palmitic acid | Arabidopsis thaliana | |
| 3.1.4.4 | oleic acid | best stimulation, optimal at 0.5 mM, stimulates binding to substrate, in presence of Ca2+ | Arabidopsis thaliana | |
| 3.1.4.4 | phosphatidylinositol-4,5-bisphosphate | less than oleic acid, best at 0.03 mM | Arabidopsis thaliana | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | - |
Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | R399P | complete loss of activity | Arabidopsis thaliana |
| 3.1.4.4 | R611D | loss of more than 80% of phosphatidylinositol-stimulated activity, 50% of oleate-stimulated activity | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | inhibitory above 1 mM | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.4 | plasma membrane | tightly associated to | Arabidopsis thaliana | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | required, optimal at 0.1 mM, inhibitory above 1 mM | Arabidopsis thaliana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 124000 | - |
x * 124000, SDS-PAGE, GST-fusion protein | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Arabidopsis thaliana | Q9C5Y0 | isozyme PLDdelta | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | flower | - |
Arabidopsis thaliana | - |
| 3.1.4.4 | leaf | higher expression in old than in young parts of plant | Arabidopsis thaliana | - |
| 3.1.4.4 | stem | - |
Arabidopsis thaliana | - |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | ? | x * 124000, SDS-PAGE, GST-fusion protein | Arabidopsis thaliana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 6 | 7 | - |
Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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