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Literature summary extracted from
- Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance (2001), J. Biol. Chem., 276, 10498-10504.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.5 | ADP | required for full activity, of the recombinant human enzyme, allosteric activator | Homo sapiens |  |
| 3.1.3.5 | ATP | activates | Homo sapiens | |
| 3.1.3.5 | GTP | serves as activitor, not as strong as ADP | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.5 | expression in Sf9 insect cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.5 | 2-fluoroadenine-9-beta-D-arabinofuranoside | 1 mM, 70% inhibition with 5 mM 5'-AMP as substrate | Homo sapiens | |
| 3.1.3.5 | araCMP | 1 mM, 25% inhibition with 5 mM 5'-AMP as substrate | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.5 | 0.012 | - |
dCMP | pH 7.0, 37°C, in presence of 1 mM ADP | Homo sapiens | |
| 3.1.3.5 | 0.193 | - |
dCMP | pH 7.0, 37°C, without ADP | Homo sapiens | |
| 3.1.3.5 | 1.87 | - |
5'-AMP | pH 7.0, 37°C, in presence of 1 mM ADP | Homo sapiens | |
| 3.1.3.5 | 2.47 | - |
5'-IMP | pH 7.0, 37°C, in presence of 1 mM ADP | Homo sapiens | |
| 3.1.3.5 | 2.96 | - |
5'-AMP | pH 7.0, 37°C, without ADP | Homo sapiens | |
| 3.1.3.5 | 12.11 | - |
5'-IMP | pH 7.0, 37°C, without ADP | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.5 | cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.5 | 47700 | - |
x * 47700, calculation from nucleotide sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.5 | additional information | Homo sapiens | cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.5 | Homo sapiens | Q9BXI3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.5 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.5 | brain | - |
Homo sapiens | - |
| 3.1.3.5 | heart | high activity | Homo sapiens | - |
| 3.1.3.5 | HEK-293 cell | - |
Homo sapiens | - |
| 3.1.3.5 | JURKAT cell | - |
Homo sapiens | - |
| 3.1.3.5 | kidney | low activity | Homo sapiens | - |
| 3.1.3.5 | pancreas | - |
Homo sapiens | - |
| 3.1.3.5 | skeletal muscle | high activity | Homo sapiens | - |
| 3.1.3.5 | testis | low activity | Homo sapiens | - |
| 3.1.3.5 | uterus | low activity | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.5 | 14.4 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.5 | 2-chloro-2'-deoxyadenosine monophosphate + H2O | 39% of the activity with 5'-AMP | Homo sapiens | 2-chloro-2'-deoxyadenosine + phosphate | - |
? | |
| 3.1.3.5 | 5'-AMP + H2O | - |
Homo sapiens | adenosine + phosphate | - |
? | |
| 3.1.3.5 | 5'-CMP + H2O | 85% of the activity with 5'-AMP | Homo sapiens | cytidine + phosphate | - |
? | |
| 3.1.3.5 | 5'-dIMP + H2O | 61% of the activity with 5'-AMP | Homo sapiens | deoxyinosine + phosphate | - |
? | |
| 3.1.3.5 | 5'-GMP + H2O | 57% of the activity with 5'-AMP | Homo sapiens | guanosine + phosphate | - |
? | |
| 3.1.3.5 | 5'-IMP + H2O | - |
Homo sapiens | inosine + phosphate | - |
? | |
| 3.1.3.5 | 5'-TMP + H2O | 13% of the activity with 5'-AMP | Homo sapiens | thymidine + phosphate | - |
? | |
| 3.1.3.5 | 5'-UMP + H2O | 78% of the activity with 5'-AMP | Homo sapiens | uridine + phosphate | - |
? | |
| 3.1.3.5 | dAMP + H2O | 91% of the activity with 5'-AMP | Homo sapiens | deoxyadenosine + phosphate | - |
? | |
| 3.1.3.5 | dCMP + H2O | 10% of the activity with 5'-AMP | Homo sapiens | deoxycytidine + phosphate | - |
? | |
| 3.1.3.5 | dGMP + H2O | 75% of the activity with 5'-AMP | Homo sapiens | deoxyguanosine + phosphate | - |
? | |
| 3.1.3.5 | dIMP + H2O | 84% of the activity with 5'-AMP | Homo sapiens | deoxyinosine + phosphate | - |
? | |
| 3.1.3.5 | dUMP + H2O | 7% of the activity with 5'-AMP | Homo sapiens | deoxyuridine + phosphate | - |
? | |
| 3.1.3.5 | additional information | no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside | Homo sapiens | ? | - |
? | |
| 3.1.3.5 | additional information | cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.5 | ? | x * 47700, calculation from nucleotide sequence | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.5 | 5'-nucleotidase I | - |
Homo sapiens |
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