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Literature summary extracted from

  • Fenster, K.M.; Parkin, K.L.; Steele, J.L.
    Characterization of an arylesterase from Lactobacillus helveticus CNRZ32 (2000), J. Appl. Microbiol., 88, 572-583.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.2 NaCl optimal concentration is 3.5% at 35°C Lactobacillus helveticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 gene estA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as C-terminally HIs-tagged protein Lactobacillus helveticus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.2 C105A site-directed mutagenesis, 55.9% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 H137A site-directed mutagenesis, 17.4% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 H164A site-directed mutagenesis, inactive mutant Lactobacillus helveticus
3.1.1.2 H56A site-directed mutagenesis, 30.0% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 S10A site-directed mutagenesis, inactive mutant Lactobacillus helveticus
3.1.1.2 S112A site-directed mutagenesis, 99.5% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 S138G site-directed mutagenesis, 99.2% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 S155A site-directed mutagenesis, 99.0% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 S42A site-directed mutagenesis, 33.2% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
3.1.1.2 S44A site-directed mutagenesis, 33.7% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 diisopropyl fluorophosphate
-
Lactobacillus helveticus
3.1.1.2 additional information no inhibition by EDTA and iodoacetic acid Lactobacillus helveticus
3.1.1.2 pepstatin A
-
Lactobacillus helveticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 22600 25100 gel filtration on Sephacryl 100 and 300 Lactobacillus helveticus
3.1.1.2 23400
-
1 * 23400, SDS-PAGE Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Lactobacillus helveticus Q9LAH7 enzyme EstA, gene estA
-
3.1.1.2 Lactobacillus helveticus CNRZ32 Q9LAH7 enzyme EstA, gene estA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 recombinant His-tagged enzyme from Escherichia coli, to homogeneity Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 additional information
-
substrate specificity Lactobacillus helveticus
3.1.1.2 486 519 purified recombinant wild-type enzyme, substrate phenyl acetate Lactobacillus helveticus
3.1.1.2 623
-
purified recombinant wild-type enzyme, substrate phenyl acetate in presence of 3.5% NaCl Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 3-acetoxypyridine + H2O
-
Lactobacillus helveticus ?
-
?
3.1.1.2 4-acetoxybenzaldehyde + H2O
-
Lactobacillus helveticus ?
-
?
3.1.1.2 4-nitrophenyl acetate + H2O
-
Lactobacillus helveticus 4-nitrophenol + acetate
-
?
3.1.1.2 4-nitrophenyl acetate + H2O
-
Lactobacillus helveticus CNRZ32 4-nitrophenol + acetate
-
?
3.1.1.2 4-nitrophenyl butyrate + H2O
-
Lactobacillus helveticus 4-nitrophenol + butyrate
-
?
3.1.1.2 4-nitrophenyl caproate + H2O
-
Lactobacillus helveticus 4-nitrophenol + caproate
-
?
3.1.1.2 4-nitrophenyl caprylate + H2O
-
Lactobacillus helveticus 4-nitrophenol + caprylate
-
?
3.1.1.2 4-nitrophenyl propionate + H2O
-
Lactobacillus helveticus 4-nitrophenol + propionate
-
?
3.1.1.2 4-nitrophenyl propionate + H2O
-
Lactobacillus helveticus CNRZ32 4-nitrophenol + propionate
-
?
3.1.1.2 4-nitrophenyl valerate + H2O
-
Lactobacillus helveticus 4-nitrophenol + valerate
-
?
3.1.1.2 additional information substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate Lactobacillus helveticus ?
-
?
3.1.1.2 additional information substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate Lactobacillus helveticus CNRZ32 ?
-
?
3.1.1.2 phenyl acetate + H2O best substrate Lactobacillus helveticus phenol + acetate
-
?
3.1.1.2 phenyl acetate + H2O best substrate Lactobacillus helveticus CNRZ32 phenol + acetate
-
?
3.1.1.2 phenylthioacetate + H2O
-
Lactobacillus helveticus thiophenol + acetate
-
?
3.1.1.2 phenylthioacetate + H2O
-
Lactobacillus helveticus CNRZ32 thiophenol + acetate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 monomer 1 * 23400, SDS-PAGE Lactobacillus helveticus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 ESt-A
-
Lactobacillus helveticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 35 40
-
Lactobacillus helveticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 7.5 8
-
Lactobacillus helveticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.2 Lactobacillus helveticus isoelectric focusing
-
8