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Literature summary extracted from

  • Binevski, P.V.; Sizova, E.A.; Pozdnev, V.F.; Kost, O.A.
    Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin-converting enzyme (2003), FEBS Lett., 550, 84-88.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 captopril
-
 Bos taurus
3.4.15.1 lisinopril
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 additional information
-
 additional information the two active sites within bovine lung enzyme exhibits strong negative cooperativity Bos taurus
3.4.15.1 0.005
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.008
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, enzyme from lung Bos taurus
3.4.15.1 0.008
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 0.05
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C, enzyme from lung Bos taurus
3.4.15.1 0.05
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Ala pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.05
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 0.12
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.13
-
 benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.14
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 0.15
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, enzyme from lung Bos taurus
3.4.15.1 0.17
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.5
-
 N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 0.6
-
 N-benzyloxycarbonyl-L-Phe-L-His-L-Leu pH 7.5, 25°C, enzyme from testis Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.1 lung
-
 Bos taurus
-
3.4.15.1 testis
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 benzyloxycarbonyl-Phe-His-Leu + H2O
-
 Bos taurus ?
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-Phe-Ala-Ala + H2O
-
 Bos taurus 2-furylacrylic acid + Phe-Ala-Ala
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys + H2O
-
 Bos taurus 2-furylacrylic acid + Phe-Ala-Lys
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro + H2O
-
 Bos taurus 2-furylacrylic acid + Phe-Ala-Pro
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly + H2O
-
 Bos taurus 2-furylacrylic acid + Phe-Gly-Gly
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg + H2O
-
 Bos taurus 2-furylacrylic acid + Phe-Phe-Arg
-
 ?
3.4.15.1 N-benzyloxycarbonyl-L-Phe-L-His-L-Leu + H2O
-
 Bos taurus N-benzyloxycarbonyl-L-Phe + L-His-L-Leu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 additional information
-
 additional information the two active sites within bovine lung enzyme exhibitsstrong negative cooperativity Bos taurus
3.4.15.1 8
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 12
-
 N-benzyloxycarbonyl-L-Phe-L-His-L-Leu pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 19
-
 benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 30
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, enzyme from lung Bos taurus
3.4.15.1 35
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 45
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Pro pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 45
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C, N-domain from lung enzyme Bos taurus
3.4.15.1 54
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, enzyme from lung Bos taurus
3.4.15.1 76
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 85
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Lys pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 178
-
 N-(3-(2-furyl)acryloyl)-Phe-Ala-Ala pH 7.5, 25°C, enzyme from testis Bos taurus
3.4.15.1 260
-
 N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C, enzyme from testis Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 0.00000012
-
 lisinopril pH 7.5, 37°C, enzyme from testis Bos taurus
3.4.15.1 0.00000018
-
 lisinopril pH 7.5, 37°C, enzyme from lung Bos taurus
3.4.15.1 0.0000002
-
 lisinopril pH 7.5, 37°C, N-domain from lung enzyme Bos taurus
3.4.15.1 0.0000005
-
 captopril pH 7.5, 37°C, N-domain from lung enzyme Bos taurus
3.4.15.1 0.0000006
-
 captopril pH 7.5, 37°C, enzyme from lung Bos taurus
3.4.15.1 0.000009
-
 captopril pH 7.5, 37°C, enzyme from testis Bos taurus