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Literature summary extracted from

  • Schutz, A.; Golbik, R.; Tittmann, K.; Svergun, D.I.; Koch, M.H.; Hubner, G.; Konig, S.
    Studies on structure-function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway (2003), Eur. J. Biochem., 270, 2322-2331.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.74 ammonium sulfate stabilizes Enterobacter cloacae
4.1.1.74 thiamine diphosphate stabilizes Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.74 0.005
-
 4-nitro-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.019
-
 4-bromo-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.02
-
 Indole-3-pyruvate pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.048
-
 4-chloro-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.111
-
 4-ethyl-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.127
-
 4-methyl-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 0.62
-
 4-fluoro-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 1.05
-
 4-methoxy-benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 1.65
-
 benzoylformic acid pH 6.5, 30°C Enterobacter cloacae
4.1.1.74 3.38
-
 pyruvate pH 6.5, 30°C Enterobacter cloacae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.74 Mg2+ required Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.74 60000
-
 4 * 60000, SDS-PAGE Enterobacter cloacae
4.1.1.74 245000
-
 gel filtration Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.74 Enterobacter cloacae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.74 recombinant enzyme from Escherichia coli Enterobacter cloacae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.74 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 mechanism Enterobacter cloacae
a

Storage Stability

EC Number Storage Stability Organism
4.1.1.74 quite stable at 40°C without further additions Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.74 3-(indol-3-yl)pyruvate
-
 Enterobacter cloacae 2-(indol-3-yl)acetaldehyde + CO2
-
 ?
4.1.1.74 4-bromo-benzoylformic acid
-
 Enterobacter cloacae 4-bromobenzaldehyde + CO2
-
 ?
4.1.1.74 4-chloro-benzoylformic acid
-
 Enterobacter cloacae 4-chlorobenzaldehyde + CO2
-
 ?
4.1.1.74 4-ethyl-benzoylformic acid
-
 Enterobacter cloacae 4-ethylbenzaldehyde + CO2
-
 ?
4.1.1.74 4-fluoro-benzoylformic acid
-
 Enterobacter cloacae 4-fluorobenzaldehyde + CO2
-
 ?
4.1.1.74 4-methoxy-benzoylformic acid
-
 Enterobacter cloacae 4-methoxybenzaldehyde + CO2
-
 ?
4.1.1.74 4-methyl-benzoylformic acid
-
 Enterobacter cloacae 4-methylbenzaldehyde + CO2
-
 ?
4.1.1.74 4-nitro-benzoylformic acid
-
 Enterobacter cloacae 4-nitrobenzaldehyde + CO2
-
 ?
4.1.1.74 benzoylformic acid
-
 Enterobacter cloacae benzaldehyde + CO2
-
 ?
4.1.1.74 pyruvate
-
 Enterobacter cloacae acetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.74 More at pH-values between pH 5.6 and 6.0 the tetrameric form dominates, at pH values above 8.0, the monomeric form is present Enterobacter cloacae
4.1.1.74 tetramer 4 * 60000, SDS-PAGE Enterobacter cloacae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.74 thiamine diphosphate
-
 Enterobacter cloacae