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Literature summary extracted from

  • Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
    Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures (2002), Eur. J. Biochem., 269, 6278-6286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ba2+ 5 mM, SAP2, 50% inhibition Solanum lycopersicum
3.1.3.2 Ca2+ 5 mM, SAP2, 60% inhibition Solanum lycopersicum
3.1.3.2 Co2+ 5 mM, SAP2, 95% inhibition Solanum lycopersicum
3.1.3.2 Cu2+ 5 mM, SAP1, 91% inhibition Solanum lycopersicum
3.1.3.2 EDTA 5 mM, SAP2, 71% inhibition Solanum lycopersicum
3.1.3.2 F-
-
 Solanum lycopersicum
3.1.3.2 Mn2+ 5 mM, SAP2, 26% inhibition Solanum lycopersicum
3.1.3.2 molybdate competitive Solanum lycopersicum
3.1.3.2 additional information SAP1 and SAP2 are not inhibited by tartrate Solanum lycopersicum
3.1.3.2 phosphate mixed and competitive Solanum lycopersicum
3.1.3.2 Zn2+ 5 mM, SAP2, complete inhibition of SAP1 and SAP2 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 1.4
-
 phosphoenolpyruvate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 1.9
-
 Tetrapolyphosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 2.1
-
 phosphoenolpyruvate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 2.1
-
 Phenyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 2.1
-
 phosphotyrosine pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 2.9
-
 Tetrapolyphosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 3.3
-
 4-nitrophenyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 3.9
-
 2-naphthyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 4.1
-
 ATP pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 4.5
-
 6-phospho-D-gluconate
-
 Solanum lycopersicum
3.1.3.2 4.5
-
 4-nitrophenyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 4.5
-
 2-naphthyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 4.6
-
 diphosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 5
-
 glycerol 3-phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 5.3
-
 Phenyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 5.3
-
 1-naphthyl phosphate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 5.4
-
 GTP pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 5.5
-
 ATP pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 6
-
 3-phosphoglycerate pH 5.3, 25°C, SAP2 Solanum lycopersicum
3.1.3.2 6.4
-
 1-naphthyl phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 8.6
-
 GTP pH 5.3, 25°C, SAP1 Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 extracellular
-
 Solanum lycopersicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ba2+ 5 mM, SAP1 is activated to 115% of control Solanum lycopersicum
3.1.3.2 EDTA 5 mM, SAP1 is activated to 114% of control Solanum lycopersicum
3.1.3.2 Mg2+ 5 mM, SAP1 is activated to 136% of control, SAP2 is activated to 181% of control Solanum lycopersicum
3.1.3.2 Mn2+ 5 mM, SAP1 is activated to 113% of control Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Solanum lycopersicum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 two secreted acid phosphatases: SAP1 and SAP2 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 cell suspension culture
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 246
-
 SAP1 Solanum lycopersicum
3.1.3.2 940
-
 SAP2 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 1-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 2-naphthol + phosphate
-
 ?
3.1.3.2 3-phosphoglycerate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum glycerate + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum 4-nitrophenol + phosphate
-
 ?
3.1.3.2 6-phospho-D-gluconate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum D-gluconate + phosphate
-
 ?
3.1.3.2 ATP + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?
3.1.3.2 diphosphate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum 2 phosphate
-
 ?
3.1.3.2 glycerol 3-phosphate + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum glycerol + phosphate
-
 ?
3.1.3.2 GTP + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?
3.1.3.2 additional information SAP1 and SAP2 are multifunctional enzymes, the enzymes also exhibit significant peroxidase activity which is optimal at approximately pH 8.4 and insensitive to Mg2+ or molybdate Solanum lycopersicum ?
-
 ?
3.1.3.2 phenyl phosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum phenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum pyruvate + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O reaction with SAP2, no activity with SAP1 Solanum lycopersicum tyrosine + phosphate
-
 ?
3.1.3.2 tetrapolyphosphate + H2O reaction with SAP1 and SAP2 Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 purple acid phosphatase
-
 Solanum lycopersicum
3.1.3.2 SAP1
-
 Solanum lycopersicum
3.1.3.2 SAP2
-
 Solanum lycopersicum
3.1.3.2 secreted acid phosphatase
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.3
-
 isoenzyme SAP1 and SAP2 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0002
-
 molybdate pH 5.3, 25°C, SAP1 or SAP2 Solanum lycopersicum
3.1.3.2 0.48
-
 F- pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 0.95
-
 F- pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 1.2
-
 phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum
3.1.3.2 1.3
-
 phosphate pH 5.3, 25°C, SAP1 Solanum lycopersicum