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Literature summary extracted from

  • Guillen, F.; Martinez, A.T.; Martinez, M.J.
    Substrate specificity and properties of the aryl-alcohol oxidase from ligninolytic fungus Pleurotus eryngii (1992), Eur. J. Biochem., 209, 603-611.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.7 3-Phenyl-1-propanol
-
 Pleurotus eryngii
1.1.3.7 phenol competitive Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 0.04
-
 4-methoxybenzyl alcohol pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 0.22
-
 3-Methoxybenzyl alcohol pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 0.41
-
 3,4-dimethoxybenzyl alcohol pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 0.79
-
 2,4-Dimethoxybenzyl alcohol pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 0.84
-
 benzyl alcohol pH 6.0, 25°C Pleurotus eryngii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.7 extracellular
-
 Pleurotus eryngii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.7 66700
-
 gel filtration Pleurotus eryngii
1.1.3.7 72600
-
 1 * 72600, SDS-PAGE Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus eryngii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 glycoprotein 15% carbohydrate content Pleurotus eryngii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.7
-
 Pleurotus eryngii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.7 79.5
-
-
 Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 2,4-dimethoxybenzyl alcohol + O2 177.5% of the activity with benzyl alcohol Pleurotus eryngii 2,4-dimethoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 2,4-hexadien-1-ol + O2 531% of the activity with benzyl alcohol Pleurotus eryngii ? + H2O2
-
 ?
1.1.3.7 2-naphthalenemethanol + O2 745.7% of the activity with benzyl alcohol Pleurotus eryngii 2-naphthaleneformaldehyde + H2O
-
 ?
1.1.3.7 3,4-dimethoxybenzyl alcohol + O2 326.1% of the activity with benzyl alcohol Pleurotus eryngii 3,4-dimethoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 3-methoxybenzyl alcohol + O2 as active as benzyl alcohol Pleurotus eryngii 3-methoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 4-methoxybenzyl alcohol + O2 571.4% of the activity with benzyl alcohol Pleurotus eryngii 4-methoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Pleurotus eryngii benzaldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2 451.1% of the activity with benzyl alcohol Pleurotus eryngii cinnamaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 monomer 1 * 72600, SDS-PAGE Pleurotus eryngii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 55
-
-
 Pleurotus eryngii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.7 50
-
 30 min, stable up to Pleurotus eryngii
1.1.3.7 60
-
 half-life: 5 min Pleurotus eryngii
1.1.3.7 65
-
 5 min, total inactivation Pleurotus eryngii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.7 1.92
-
 phenol pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 4.48
-
 3-Phenyl-1-propanol pH 6.0, 25°C Pleurotus eryngii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.7 Pleurotus eryngii isoelectric focusing, pH-gradient: 2.5-5.0
-
 3.9