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Literature summary extracted from

  • Yan, G.; Cheng, S.; Zhao, G.; Wu, S.; Liu, Y.; Sun, W.
    A single residual replacement improves the folding and stability of recombinant cassava hydroxynitrile lyase in E. coli (2003), Biotechnol. Lett., 25, 1041-1047.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 expressed in Escherichia coli BL21 (DE3) Manihot esculenta

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.47 G113S enhanced thermal stability compared to wild-type enzyme, mutant enzyme retains slight higher activity than the wild-type enzyme in an acidic environment, so the mutant enzyme maybe more effective for synthesis of (S)-cyanohydrin than the wild-type enzyme Manihot esculenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 1.1
-
 mandelonitrile pH 5.4, 25°C, mutant enzyme G113S Manihot esculenta
4.1.2.47 1.4
-
 mandelonitrile pH 5.4, 25°C, wild-type enzyme Manihot esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 29000
-
 4 * 29000, SDS-PAGE Manihot esculenta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.47 mandelonitrile Manihot esculenta
-
 HCN + benzaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.47 recombinant enzyme Manihot esculenta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.47 35.6
-
 wild-type enzyme Manihot esculenta
4.1.2.47 57.2
-
 mutant enzyme G113S Manihot esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 mandelonitrile
-
 Manihot esculenta HCN + benzaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.47 tetramer 4 * 29000, SDS-PAGE Manihot esculenta

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-HNL
-
 Manihot esculenta
4.1.2.47 HNL
-
 Manihot esculenta
4.1.2.47 HNL1
-
 Manihot esculenta
4.1.2.47 Hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 MeHNL
-
 Manihot esculenta
4.1.2.47 mut-HNL1
-
 Manihot esculenta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.47 25
-
-
 Manihot esculenta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.47 25 70
-
 Manihot esculenta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.47 45
-
 mutant enzyme and wild-type enzyme G113S are stable for 20 min at pH 5.4 Manihot esculenta
4.1.2.47 60
-
 20 min at pH 5.4, mutant enzyme G113S loses about 10% of its initial activity, wild-type enzyme loses about 20% of its initial activity. More than 50% loss of activity of wild-type enzyme after 50 min, 10% loss of activity of mutant enzyme G113 after 50 min, 75% loss of activity of wild-type enzyme after 120 min, 25% loss of activity of mutant enzyme G113 after 120 min Manihot esculenta
4.1.2.47 65
-
 20 min at pH 5.4, mutant enzyme G113S loses about 15% of its initial activity, wild-type enzyme loses about 50% of its initial activity Manihot esculenta
4.1.2.47 70
-
 20 min at pH 5.4, about 80% loss of activity, mutant enzyme G113S and wild-type enzyme Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.47 5.5
-
 mutant enzyme G113S Manihot esculenta
4.1.2.47 5.6
-
 wild-type enzyme Manihot esculenta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.47 4.5 6.5 pH 4.5: about 65% of maximal activity of mutant enzyme G113S, about 60% of maximal activity of wild-type enzyme, pH 6.5: about 50% of maximal activity of mutant enzyme G113S, about 55% of maximal activity of wild-type enzyme Manihot esculenta

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.47 additional information FAD-independent Manihot esculenta