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Literature summary extracted from

  • Nakayama, N.; Matsubara, T.; Ohshiro, T.; Moroto, Y.; Kawata, Y.; Koizumi, K.; Hirakawa, Y.; Suzuki, M.; Maruhashi, K.; Izumi, Y.; Kurane, R.
    A novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterization (2002), Biochim. Biophys. Acta, 1598, 122-130.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.1.3 additional information no activation by metal ions Rhodococcus erythropolis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.3 gene dszZ, DNA sequence determination, cooverexpression in Escherichia coli with chaperonin genes groEL and groES at 25°C Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
3.13.1.3 C26S site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.1.3 2,2'-Dihydroxybiphenyl competitive Rhodococcus erythropolis
3.13.1.3 2,3-Dihydroxybiphenyl competitive Rhodococcus erythropolis
3.13.1.3 2-Hydroxybiphenyl competitive, product inhibition Rhodococcus erythropolis
3.13.1.3 Ag+ strong inhibition at 1 mM Rhodococcus erythropolis
3.13.1.3 Cu2+ strong inhibition at 1 mM Rhodococcus erythropolis
3.13.1.3 DTNB strong inhibition at 1 mM Rhodococcus erythropolis
3.13.1.3 Hg2+ strong inhibition at 1 mM Rhodococcus erythropolis
3.13.1.3 additional information no inhibition by product sulfite, no inhibition by chelating agents EDTA, 1,10-phenanthroline, and 8-hydroxyquinone Rhodococcus erythropolis
3.13.1.3 N-ethylmaleimide strong inhibition at 1 mM Rhodococcus erythropolis
3.13.1.3 p-chloromercuribenzoate strong inhibition at 1 mM Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.3 0.0082
-
 2'-hydroxybiphenyl-2-sulfinate pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 0.062
-
 2-phenylbenzenesulfinate pH 7.0, 28°C Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.13.1.3 additional information no metal ions required Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.1.3 40000
-
 1 * 40000, SDS-PAGE Rhodococcus erythropolis
3.13.1.3 43000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O Rhodococcus erythropolis
-
 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O Rhodococcus erythropolis KA2-5-1
-
 2-hydroxybiphenyl + sulfite
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.3 Rhodococcus erythropolis
-
-
-
3.13.1.3 Rhodococcus erythropolis KA2-5-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.3 recombinant 7.8fold from Escherichia coli, and native from strain KA2-5-1 160fold, to homogeneity Rhodococcus erythropolis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O = 2-hydroxybiphenyl + sulfite Cys26 is essential for activity Rhodococcus erythropolis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.1.3 0.18
-
 purified native enzyme Rhodococcus erythropolis
3.13.1.3 0.207
-
 purified recombinant enzyme Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O highly specific for Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis KA2-5-1 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O highly specific for Rhodococcus erythropolis KA2-5-1 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2-phenylbenzenesulfinate + H2O
-
 Rhodococcus erythropolis biphenyl + sulfite
-
 ?
3.13.1.3 2-phenylbenzenesulfinate + H2O
-
 Rhodococcus erythropolis KA2-5-1 biphenyl + sulfite
-
 ?

Subunits

EC Number Subunits Comment Organism
3.13.1.3 monomer 1 * 40000, SDS-PAGE Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate desulfinase
-
 Rhodococcus erythropolis
3.13.1.3 DszB
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.3 35
-
 recombinant enzyme Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.1.3 28
-
 recombinant enzyme, stable Rhodococcus erythropolis
3.13.1.3 30
-
 recombinant enzyme, rapid loss of activity above, inactivation after 4 h Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.3 7.5
-
 about, recombinant enzyme Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.3 additional information no cofactor required Rhodococcus erythropolis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.13.1.3 0.25
-
 2-Hydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis
3.13.1.3 0.4
-
 2,3-Dihydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis
3.13.1.3 2
-
 2,2'-Dihydroxybiphenyl pH 7.0, 28°C, recombinant enzyme Rhodococcus erythropolis