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Literature summary extracted from

  • Garats, E.V.; Nikolskaya, II; Binevski, P.V.; Pozdnev, V.F.; Kost, O.A.
    Characterization of bovine atrial angiotensin-converting enzyme (2001), Biochemistry (Moscow), 66, 429-434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.15.1 Cl- activates Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 captopril
-
 Bos taurus
3.4.15.1 lisinopril
-
 Bos taurus
3.4.15.1 phosphoramidon weak, IC50: 0.001 mM Bos taurus
3.4.15.1 thiorphan weak, IC50: 0.0001 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 0.1
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C Bos taurus
3.4.15.1 0.14
-
 benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C Bos taurus
3.4.15.1 0.98
-
 N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C Bos taurus
3.4.15.1 1.8
-
 N-hippuryl-His-Leu pH 7.5, 25°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.1 180000
-
 x * 180000, electrophoresis under denaturing conditions Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.1 atrial tissue
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.15.1 0.37
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.15.1 -20°C, 90% loss of activity after 1 month Bos taurus
3.4.15.1 4°C, pH 7.2, in presence of 10% glycerol, activity remains unchanged for at least 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 benzyloxycarbonyl-Phe-His-Leu + H2O
-
 Bos taurus ?
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-L-Phe-Gly-Gly + H2O
-
 Bos taurus 2-furylacrylic acid + L-Phe-Gly-Gly
-
 ?
3.4.15.1 N-(3-(2-furyl)acryloyl)-L-Phe-Phe-Arg + H2O
-
 Bos taurus 2-furylacrylic acid + L-Phe-Phe-Arg
-
 ?
3.4.15.1 N-hippuryl-His-Leu + H2O
-
 Bos taurus hippuric acid + His-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.15.1 ? x * 180000, electrophoresis under denaturing conditions Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 6.5
-
 N-hippuryl-His-Leu pH 7.5, 25°C Bos taurus
3.4.15.1 30
-
 N-(3-(2-furyl)acryloyl)-Phe-Phe-Arg pH 7.5, 25°C Bos taurus
3.4.15.1 100
-
 benzyloxycarbonyl-Phe-His-Leu pH 7.5, 25°C Bos taurus
3.4.15.1 315
-
 N-(3-(2-furyl)acryloyl)-Phe-Gly-Gly pH 7.5, 25°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.15.1 7.2
-
 reaction with benzyloxycarbonyl-Phe-His-Leu Bos taurus
3.4.15.1 8.3
-
 reaction with N-hippuryl-His-Leu Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.15.1 6 8.5 pH 6.0: about 40% of maximal activity, pH 8.5: about 35% of maximal activity, reaction with benzyloxycarbonyl-Phe-His-Leu Bos taurus
3.4.15.1 7 9.5 pH 7.0: about 40% of maximal activity, pH 9.5: about 50% of maximal activity, reaction with N-hippuryl-His-Leu Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 0.0000003
-
 captopril pH 7.5, 25°C Bos taurus
3.4.15.1 0.0000006
-
 lisinopril pH 7.5, 25°C Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.15.1 0.0001
-
 weak, IC50: 0.0001 mM Bos taurus thiorphan
3.4.15.1 0.001
-
 weak, IC50: 0.001 mM Bos taurus phosphoramidon