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Literature summary extracted from

  • Deng, S.; Elkins, J.G.; Da, L.H.; Botero, L.M.; McDermott, T.R.
    Cloning and characterization of a second acid phosphatase from Sinorhizobium meliloti strain 104A14 (2001), Arch. Microbiol., 176, 255-263.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2
-
 Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.194
-
 4-nitrophenyl phosphate pH 4.8, 37°C Sinorhizobium meliloti
3.1.3.2 1.06
-
 Alpha-naphthyl phosphate pH 4.8, 37°C Sinorhizobium meliloti
3.1.3.2 2.59
-
 Phenyl phosphate pH 4.8, 37°C Sinorhizobium meliloti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 periplasm
-
 Sinorhizobium meliloti
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 35800
-
 2 * 35800, SDS-PAGE Sinorhizobium meliloti
3.1.3.2 70000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Sinorhizobium meliloti
-
-
-
3.1.3.2 Sinorhizobium meliloti 104A14
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 partial Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 70
-
-
 Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Sinorhizobium meliloti 1-naphthol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Sinorhizobium meliloti 4-nitrophenol + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Sinorhizobium meliloti adenosine + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Sinorhizobium meliloti 104A14 adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Sinorhizobium meliloti ?
-
 ?
3.1.3.2 ATP + H2O
-
 Sinorhizobium meliloti 104A14 ?
-
 ?
3.1.3.2 bis-p-nitrophenyl phosphate + H2O
-
 Sinorhizobium meliloti p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O
-
 Sinorhizobium meliloti D-ribose + phosphate
-
 ?
3.1.3.2 D-ribose 5-phosphate + H2O
-
 Sinorhizobium meliloti 104A14 D-ribose + phosphate
-
 ?
3.1.3.2 DL-myo-inositol-1-monophosphate + H2O
-
 Sinorhizobium meliloti DL-myo-inositol + phosphate
-
 ?
3.1.3.2 additional information no activity detected with: DL-myo-inositol-hexaphosphate, D-glucose 6-phosphate, O-phospho-DL-serine, O-phospho-DL-tyrosine and O-phospho-DL-threonine Sinorhizobium meliloti ?
-
 ?
3.1.3.2 additional information no activity detected with: DL-myo-inositol-hexaphosphate, D-glucose 6-phosphate, O-phospho-DL-serine, O-phospho-DL-tyrosine and O-phospho-DL-threonine Sinorhizobium meliloti 104A14 ?
-
 ?
3.1.3.2 phenolphthalein diphosphate + H2O
-
 Sinorhizobium meliloti ?
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Sinorhizobium meliloti phenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Sinorhizobium meliloti 104A14 phenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 35800, SDS-PAGE Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 NapE
-
 Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5 5 reaction with 4-nitrophenyl phosphate Sinorhizobium meliloti
3.1.3.2 6.5
-
 reaction with phenyl phosphate Sinorhizobium meliloti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 2.5 6 pH 2.0: about 25% of maximal activity, pH 2.5: 65% of maximal activity, pH 6.0: about 40% of maximal activity, reaction with 4-nitrophenyl phosphate Sinorhizobium meliloti
3.1.3.2 6 7 pH 6.0: about 40% of maximal activity, pH 7.0: about 35% of maximal activity, reaction with phenyl phosphate Sinorhizobium meliloti