EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | 2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone | 0.2 mM: 50% inhibition | Pseudomonas fluorescens | |
4.1.2.38 | Desoxyanisoin | 0.4 mM: 50% inhibition | Pseudomonas fluorescens | |
4.1.2.38 | desoxybenzoin | 0.9 mM: 50% inhibition | Pseudomonas fluorescens | |
4.1.2.38 | additional information | benzoylformic acid at 0.9 mM, pyruvic acid at 10 mM, acetoin at 10 mM, N-ethylmaleimide at 20 mM, dithiothreitol at 5 mM are no inhibitors | Pseudomonas fluorescens | |
4.1.2.38 | thiamine diphosphate | more than 0.5 mM: inhibition, requirement for 0.01 mM | Pseudomonas fluorescens | |
4.1.2.38 | Urea | 4 mM: inactivation | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.38 | 0.009 | - |
Benzoin | 37°C, pH 8 | Pseudomonas fluorescens | |
4.1.2.38 | 0.0325 | - |
Anisoin | 37°C, pH 8 | Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | Ba2+ | less effective than Mg2+ or Mn2+ or Ca2+ | Pseudomonas fluorescens | |
4.1.2.38 | Ca2+ | 1 mM, absolute requirement of a divalent cation, may be substituted by Mn2+ or Mg2+ | Pseudomonas fluorescens | |
4.1.2.38 | Cu2+ | less effective than Mg2+ or Mn2+ or Ca2+ | Pseudomonas fluorescens | |
4.1.2.38 | Mg2+ | 1 mM, absolute requirement for a divalent cation, may be substituted by Mn2+ or Ca2+ | Pseudomonas fluorescens | |
4.1.2.38 | Mn2+ | 1 mM, absolute requirement for a divalent cation, may be substituted by Mg2+ or Ca2+ | Pseudomonas fluorescens | |
4.1.2.38 | additional information | Co2+, Zn2+, Ni2+, Fe2+, Al2+ at 1 mM are ineffective. KCl, NaCl, NH4Cl at 1-160 mM: no influence | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | 53000 | - |
x * 53000, SDS-PAGE | Pseudomonas fluorescens |
4.1.2.38 | 80000 | - |
gel filtration | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.38 | Pseudomonas fluorescens | - |
strain biovar I | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.38 | strain biovar I, partial | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | 11690 | - |
- |
Pseudomonas fluorescens |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.2.38 | -20°C, purified enzyme, stable for at least 2 months | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.38 | anisoin | - |
Pseudomonas fluorescens | 4-methoxybenzaldehyde + 4-methoxybenzaldehyde | - |
? | |
4.1.2.38 | benzoin | - |
Pseudomonas fluorescens | benzaldehyde + benzaldehyde | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.38 | ? | x * 53000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | 37 | - |
assay at | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | 7.5 | 8.5 | broad | Pseudomonas fluorescens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | 6 | 8.5 | no activity at pH 6 and below, maximal activity at pH 7.5-8.5 | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | thiamine diphosphate | absolute requirement, 0.01 mM optimal, more than 0.5 mM: inhibition | Pseudomonas fluorescens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.38 | 0.2 | - |
2-Acetoxy-1,2-bis(4'-methoxyphenyl)-ethanone | 37°C, pH 8 | Pseudomonas fluorescens | |
4.1.2.38 | 0.4 | - |
Desoxyanisoin | 37°C, pH 8 | Pseudomonas fluorescens | |
4.1.2.38 | 0.9 | - |
desoxybenzoin | 37°C, pH 8 | Pseudomonas fluorescens |