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Literature summary extracted from

  • Mozhaev, V.V.; Khmelnitsky, Y.L.; Sanchez-Riera, F.; Maurina-Brunker, J.; Rosson, R.A.; Grund, A.D.
    Arylsulfotransferase from Clostridium innocuum-A new enzyme catalyst for sulfation of phenol-containing compounds (2002), Biotechnol. Bioeng., 78, 567-575.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 80000
-
 4 * 80000 [Clostridium] innocuum
2.8.2.22 320000
-
-
 [Clostridium] innocuum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.8.2.22 2-pentanone enzyme activity in biphasic systems with more polar solvents gets completely lost [Clostridium] innocuum
2.8.2.22 acetonitrile enzyme tolerates presence of 10% of polar cosolvents but loses significant activity at higher solvent concentrations of 30-40% [Clostridium] innocuum
2.8.2.22 butyl acetate enzyme activity in biphasic systems with more polar solvents gets completely lost [Clostridium] innocuum
2.8.2.22 chloroform retains high enzyme activity in biphasic systems composed of water and nonpolar solvents [Clostridium] innocuum
2.8.2.22 cyclohexane retains high enzyme activity in biphasic systems composed of water and nonpolar solvents [Clostridium] innocuum
2.8.2.22 dimethyl formamide enzyme tolerates presence of 10% of polar cosolvents but loses significant activity at higher solvent concentrations of 30-40% [Clostridium] innocuum
2.8.2.22 Ethyl acetate enzyme activity in biphasic systems with more polar solvents gets completely lost [Clostridium] innocuum
2.8.2.22 Methanol enzyme tolerates presence of 10% of polar cosolvents but loses significant activity at higher solvent concentrations of 30-40% [Clostridium] innocuum
2.8.2.22 methyl tert-butyl ether enzyme activity in biphasic systems with more polar solvents gets completely lost [Clostridium] innocuum
2.8.2.22 toluene retains high enzyme activity in biphasic systems composed of water and nonpolar solvents [Clostridium] innocuum

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 [Clostridium] innocuum
-
-
-
2.8.2.22 [Clostridium] innocuum 554
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22
-
 [Clostridium] innocuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 [Clostridium] innocuum phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 [Clostridium] innocuum 554 phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 additional information enzyme does not react with 3-phosphoadenosine-5'-phosphosulfate, primary alcohols, secondary alcohols and sugars [Clostridium] innocuum ?
-
 ?
2.8.2.22 additional information enzyme does not react with 3-phosphoadenosine-5'-phosphosulfate, primary alcohols, secondary alcohols and sugars [Clostridium] innocuum 554 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 tetramer 4 * 80000 [Clostridium] innocuum