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Literature summary extracted from

  • Lee, N.S.; Kim, B.T.; Kim, D.H.; Kobashi, K.
    Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium (1995), J. Biochem., 118, 796-801.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 1-naphthyl sulfate competitive with 4-nitrophenyl sulfate, noncompetitive with 1-naphthol Haemophilus sp.
2.8.2.22 4-nitrophenol noncompetitive with respect to 4-nitrophenyl sulfate, competitive with respect to alpha-naphthol Haemophilus sp.
2.8.2.22 Cd2+ 2 mM Haemophilus sp.
2.8.2.22 Co2+ slight inhibition at 2 mM Haemophilus sp.
2.8.2.22 Cu2+ slight inhibition at 2 mM Haemophilus sp.
2.8.2.22 Ni2+ 2 mM Haemophilus sp.
2.8.2.22 Zn2+ 2 mM Haemophilus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.22 0.095
-
 4-nitrophenyl sulfate pH 10, 37°C, with phenol as second substrate Haemophilus sp.
2.8.2.22 0.71
-
 phenol pH 10, 37°C, with 4-nitrophenyl sulfate as second substrate Haemophilus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 70000
-
 4 * 70000, SDS-PAGE Haemophilus sp.
2.8.2.22 290000
-
 gel filtration Haemophilus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Haemophilus sp.
-
 K-12
-
2.8.2.22 Haemophilus sp. K-12
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 homogeneity Haemophilus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.22 an aryl sulfate + a phenol = a phenol + an aryl sulfate mechanism Haemophilus sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.22 6.67
-
-
 Haemophilus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 2-naphthyl sulfate + phenol
-
 Haemophilus sp. 2-naphthol + phenyl sulfate
-
 ?
2.8.2.22 2-naphthyl sulfate + phenol
-
 Haemophilus sp. K-12 2-naphthol + phenyl sulfate
-
 ?
2.8.2.22 4-methylumbelliferyl sulfate + phenol
-
 Haemophilus sp. 4-methylumbelliferol + phenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol
-
 Haemophilus sp. 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol
-
 Haemophilus sp. K-12 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Haemophilus sp. phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Haemophilus sp. K-12 phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + resorcinol
-
 Haemophilus sp. resorcinol O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + resorcinol
-
 Haemophilus sp. K-12 resorcinol O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 alpha-naphthyl sulfate + phenol
-
 Haemophilus sp. alpha-naphthol + phenyl sulfate
-
 ?
2.8.2.22 alpha-naphthyl sulfate + phenol
-
 Haemophilus sp. K-12 alpha-naphthol + phenyl sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 tetramer 4 * 70000, SDS-PAGE Haemophilus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.22 0.12
-
 4-nitrophenol pH 9, 37°C, for alpha-naphthol Haemophilus sp.
2.8.2.22 0.89
-
 4-nitrophenol pH 9, 37°C, for 4-nitrophenyl sulfate Haemophilus sp.
2.8.2.22 1.7
-
 1-naphthyl sulfate pH 9, 37°C, for 1-naphthol Haemophilus sp.
2.8.2.22 2.72
-
 1-naphthyl sulfate pH 9, 37°C, for 4-nitrophenyl sulfate Haemophilus sp.